This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Taboga, O.
Right arrow Articles by Palma, E. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taboga, O.
Right arrow Articles by Palma, E. L.

 Previous Article  |  Next Article 

J. Virol., Apr 1997, 2606-2614, Vol 71, No. 4
Copyright © 1997, American Society for Microbiology

A large-scale evaluation of peptide vaccines against foot-and-mouth disease: lack of solid protection in cattle and isolation of escape mutants

O Taboga, C Tami, E Carrillo, JI Nunez, A Rodriguez, JC Saiz, E Blanco, ML Valero, X Roig, JA Camarero, D Andreu, MG Mateu, E Giralt, E Domingo, F Sobrino and EL Palma
Instituto de Biotecnologia, Centro de Investigacion en Ciencias Veterinarias, INTA, Buenos Aires, Argentina.

A large-scale vaccination experiment involving a total of 138 cattle was carried out to evaluate the potential of synthetic peptides as vaccines against foot-and-mouth disease. Four types of peptides representing sequences of foot-and-mouth disease virus (FMDV) C3 Argentina 85 were tested: A, which includes the G-H loop of capsid protein VP1 (site A); AT, in which a T-cell epitope has been added to site A; AC, composed of site A and the carboxy-terminal region of VP1 (site C); and ACT, in which the three previous capsid motifs are colinearly represented. Induction of neutralizing antibodies, lymphoproliferation in response to viral antigens, and protection against challenge with homologous infectious virus were examined. None of the tested peptides, at several doses and vaccination schedules, afforded protection above 40%. Protection showed limited correlation with serum neutralization activity and lymphoproliferation in response to whole virus. In 12 of 29 lesions from vaccinated cattle that were challenged with homologous virus, mutant FMDVs with amino acid substitutions at antigenic site A were identified. This finding suggests the rapid generation and selection of FMDV antigenic variants in vivo. In contrast with previous studies, this large-scale vaccination experiment with an important FMDV host reveals considerable difficulties for vaccines based on synthetic peptides to achieve the required levels of efficacy. Possible modifications of the vaccine formulations to increase protective activity are discussed.


This article has been cited by other articles:

  • Paton, D. J., Sumption, K. J., Charleston, B. (2009). Options for control of foot-and-mouth disease: knowledge, capability and policy. Phil Trans R Soc B 364: 2657-2667 [Abstract] [Full Text]  
  • Juleff, N., Windsor, M., Lefevre, E. A., Gubbins, S., Hamblin, P., Reid, E., McLaughlin, K., Beverley, P. C. L., Morrison, I. W., Charleston, B. (2009). Foot-and-Mouth Disease Virus Can Induce a Specific and Rapid CD4+ T-Cell-Independent Neutralizing and Isotype Class-Switched Antibody Response in Naive Cattle. J. Virol. 83: 3626-3636 [Abstract] [Full Text]  
  • Rodriguez Pulido, M., Sobrino, F., Borrego, B., Saiz, M. (2009). Attenuated Foot-and-Mouth Disease Virus RNA Carrying a Deletion in the 3' Noncoding Region Can Elicit Immunity in Swine. J. Virol. 83: 3475-3485 [Abstract] [Full Text]  
  • Gutierrez-Rivas, M., Pulido, M. R., Baranowski, E., Sobrino, F., Saiz, M. (2008). Tolerance to mutations in the foot-and-mouth disease virus integrin-binding RGD region is different in cultured cells and in vivo and depends on the capsid sequence context. J. Gen. Virol. 89: 2531-2539 [Abstract] [Full Text]  
  • Cubillos, C., de la Torre, B. G., Jakab, A., Clementi, G., Borras, E., Barcena, J., Andreu, D., Sobrino, F., Blanco, E. (2008). Enhanced Mucosal Immunoglobulin A Response and Solid Protection against Foot-and-Mouth Disease Virus Challenge Induced by a Novel Dendrimeric Peptide. J. Virol. 82: 7223-7230 [Abstract] [Full Text]  
  • Nunez, J. I., Molina, N., Baranowski, E., Domingo, E., Clark, S., Burman, A., Berryman, S., Jackson, T., Sobrino, F. (2007). Guinea Pig-Adapted Foot-and-Mouth Disease Virus with Altered Receptor Recognition Can Productively Infect a Natural Host. J. Virol. 81: 8497-8506 [Abstract] [Full Text]  
  • Rieder, E., Henry, T., Duque, H., Baxt, B. (2005). Analysis of a Foot-and-Mouth Disease Virus Type A24 Isolate Containing an SGD Receptor Recognition Site In Vitro and Its Pathogenesis in Cattle. J. Virol. 79: 12989-12998 [Abstract] [Full Text]  
  • Zhao, X., Sullender, W. M. (2005). In Vivo Selection of Respiratory Syncytial Viruses Resistant to Palivizumab. J. Virol. 79: 3962-3968 [Abstract] [Full Text]  
  • Duque, H., LaRocco, M., Golde, W. T., Baxt, B. (2004). Interactions of Foot-and-Mouth Disease Virus with Soluble Bovine {alpha}V{beta}3 and {alpha}V{beta}6 Integrins. J. Virol. 78: 9773-9781 [Abstract] [Full Text]  
  • Ruiz-Jarabo, C. M., Pariente, N., Baranowski, E., Davila, M., Gomez-Mariano, G., Domingo, E. (2004). Expansion of host-cell tropism of foot-and-mouth disease virus despite replication in a constant environment. J. Gen. Virol. 85: 2289-2297 [Abstract] [Full Text]  
  • Grubman, M. J., Baxt, B. (2004). Foot-and-Mouth Disease. Clin. Microbiol. Rev. 17: 465-493 [Abstract] [Full Text]  
  • Gonzalez-Lopez, C., Arias, A., Pariente, N., Gomez-Mariano, G., Domingo, E. (2004). Preextinction Viral RNA Can Interfere with Infectivity. J. Virol. 78: 3319-3324 [Abstract] [Full Text]  
  • Hohlich, B.-J., Wiesmuller, K.-H., Haas, B., Gerner, W., Correa, R., Hehnen, H.-R., Schlapp, T., Pfaff, E., Saalmuller, A. (2003). Induction of an antigen-specific immune response and partial protection of cattle against challenge infection with foot-and-mouth disease virus (FMDV) after lipopeptide vaccination with FMDV-specific B-cell epitopes. J. Gen. Virol. 84: 3315-3324 [Abstract] [Full Text]  
  • Baranowski, E., Molina, N., Nunez, J. I., Sobrino, F., Saiz, M. (2003). Recovery of Infectious Foot-and-Mouth Disease Virus from Suckling Mice after Direct Inoculation with In Vitro-Transcribed RNA. J. Virol. 77: 11290-11295 [Abstract] [Full Text]  
  • Fischer, D., Rood, D., Barrette, R. W., Zuwallack, A., Kramer, E., Brown, F., Silbart, L. K. (2003). Intranasal Immunization of Guinea Pigs with an Immunodominant Foot-and-Mouth Disease Virus Peptide Conjugate Induces Mucosal and Humoral Antibodies and Protection against Challenge. J. Virol. 77: 7486-7491 [Abstract] [Full Text]  
  • Zhao, Q., Pacheco, J. M., Mason, P. W. (2003). Evaluation of Genetically Engineered Derivatives of a Chinese Strain of Foot-and-Mouth Disease Virus Reveals a Novel Cell-Binding Site Which Functions in Cell Culture and in Animals. J. Virol. 77: 3269-3280 [Abstract] [Full Text]  
  • Duque, H., Baxt, B. (2003). Foot-and-Mouth Disease Virus Receptors: Comparison of Bovine {alpha}V Integrin Utilization by Type A and O Viruses. J. Virol. 77: 2500-2511 [Abstract] [Full Text]  
  • Lopez-Bueno, A., Mateu, M. G., Almendral, J. M. (2003). High Mutant Frequency in Populations of a DNA Virus Allows Evasion from Antibody Therapy in an Immunodeficient Host. J. Virol. 77: 2701-2708 [Abstract] [Full Text]  
  • Tami, C., Taboga, O., Berinstein, A., Nunez, J. I., Palma, E. L., Domingo, E., Sobrino, F., Carrillo, E. (2002). Evidence of the Coevolution of Antigenicity and Host Cell Tropism of Foot-and-Mouth Disease Virus In Vivo. J. Virol. 77: 1219-1226 [Abstract] [Full Text]  
  • Matthews, L. J., Davis, R., Smith, G. P. (2002). Immunogenically Fit Subunit Vaccine Components Via Epitope Discovery from Natural Peptide Libraries. J. Immunol. 169: 837-846 [Abstract] [Full Text]  
  • Aggarwal, N., Barnett, P. V. (2002). Antigenic sites of foot-and-mouth disease virus (FMDV): an analysis of the specificities of anti-FMDV antibodies after vaccination of naturally susceptible host species. J. Gen. Virol. 83: 775-782 [Abstract] [Full Text]  
  • Blanco, E., Garcia-Briones, M., Sanz-Parra, A., Gomes, P., De Oliveira, E., Valero, M. L., Andreu, D., Ley, V., Sobrino, F. (2001). Identification of T-Cell Epitopes in Nonstructural Proteins of Foot-and-Mouth Disease Virus. J. Virol. 75: 3164-3174 [Abstract] [Full Text]  
  • Ochoa, W. F., Kalko, S. G., Mateu, M. G., Gomes, P., Andreu, D., Domingo, E., Fita, I., Verdaguer, N. (2000). A multiply substituted G-H loop from foot-and-mouth disease virus in complex with a neutralizing antibody: a role for water molecules. J. Gen. Virol. 81: 1495-1505 [Abstract] [Full Text]  
  • Blanco, E., McCullough, K., Summerfield, A., Fiorini, J., Andreu, D., Chiva, C., Borrás, E., Barnett, P., Sobrino, F. (2000). Interspecies Major Histocompatibility Complex-Restricted Th Cell Epitope on Foot-and-Mouth Disease Virus Capsid Protein VP4. J. Virol. 74: 4902-4907 [Abstract] [Full Text]  
  • Ruiz-Jarabo, C. M., Sevilla, N., Dávila, M., Gómez-Mariano, G., Baranowski, E., Domingo, E. (1999). Antigenic properties and population stability of a foot-and-mouth disease virus with an altered Arg-Gly-Asp receptor-recognition motif. J. Gen. Virol. 80: 1899-1909 [Abstract] [Full Text]  
  • Sanz-Parra, A, Vazquez, B, Sobrino, F, Cox, S., Ley, V, Salt, J. (1999). Evidence of partial protection against foot-and-mouth disease in cattle immunized with a recombinant adenovirus vector expressing the precursor polypeptide (P1) of foot-and-mouth disease virus capsid proteins. J. Gen. Virol. 80: 671-679 [Abstract]  
  • Escarmis, C., Carrillo, E. C., Ferrer, M., Arriaza, J. F. G., Lopez, N., Tami, C., Verdaguer, N., Domingo, E., Franze-Fernandez, M. T. (1998). Rapid Selection in Modified BHK-21 Cells of a Foot-and-Mouth Disease Virus Variant Showing Alterations in Cell Tropism. J. Virol. 72: 10171-10179 [Abstract] [Full Text]  
  • Verdaguer, N., Sevilla, N., Valero, M. L., Stuart, D., Brocchi, E., Andreu, D., Giralt, E., Domingo, E., Mateu, M. G., Fita, I. (1998). A Similar Pattern of Interaction for Different Antibodies with a Major Antigenic Site of Foot-and-Mouth Disease Virus: Implications for Intratypic Antigenic Variation. J. Virol. 72: 739-748 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»