This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hirsch, V.
Right arrow Articles by Montefiori, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hirsch, V.
Right arrow Articles by Montefiori, D. C.

 Previous Article  |  Next Article 

J. Virol., Feb 1997, 1608-1620, Vol 71, No. 2
Copyright © 1997, American Society for Microbiology

A molecularly cloned, pathogenic, neutralization-resistant simian immunodeficiency virus, SIVsmE543-3

V Hirsch, D Adger-Johnson, B Campbell, S Goldstein, C Brown, WR Elkins and DC Montefiori
Immunodeficiency Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Rockville, Maryland 20852, USA. vhirsch@pop.niaid.nih.gov

An infectious molecular clone of simian immunodeficiency virus SIVsm was derived from a biological isolate obtained late in disease from an immunodeficient rhesus macaque (E543) with SIV-induced encephalitis. The molecularly cloned virus, SIVsmE543-3, replicated well in macaque peripheral blood mononuclear cells and monocyte-derived macrophages and resisted neutralization by heterologous sera which broadly neutralized genetically diverse SIV variants in vitro. SIVsmE543-3 was infectious and induced AIDS when inoculated intravenously into pig-tailed macaques (Macaca nemestrina). Two of four infected macaques developed no measurable SIV-specific antibody and succumbed to a wasting syndrome and SIV-induced meningoencephalitis by 14 and 33 weeks postinfection. The other two macaques developed antibodies reactive in Western blot and virus neutralization assays. One macaque was sacrificed at 1 year postinoculation, and the survivor has evidence of immunodeficiency, characterized by persistently low CD4 lymphocyte subsets in the peripheral blood. Plasma samples from these latter animals neutralized SIVsmE543-3 but with much lower efficiency than neutralization of other related SIV strains, confirming the difficulty by which this molecularly cloned virus is neutralized in vitro. SIVsmE543-3 will provide a valuable reagent for studying SIV-induced encephalitis, mapping determinants of neutralization, and determining the in vivo significance of resistance to neutralization in vitro.


This article has been cited by other articles:

  • Mansfield, K., Lang, S. M., Gauduin, M.-C., Sanford, H. B., Lifson, J. D., Johnson, R. P., Desrosiers, R. C. (2008). Vaccine Protection by Live, Attenuated Simian Immunodeficiency Virus in the Absence of High-Titer Antibody Responses and High-Frequency Cellular Immune Responses Measurable in the Periphery. J. Virol. 82: 4135-4148 [Abstract] [Full Text]  
  • Borda, J. T., Alvarez, X., Mohan, M., Hasegawa, A., Bernardino, A., Jean, S., Aye, P., Lackner, A. A. (2008). CD163, a Marker of Perivascular Macrophages, Is Up-Regulated by Microglia in Simian Immunodeficiency Virus Encephalitis after Haptoglobin-Hemoglobin Complex Stimulation and Is Suggestive of Breakdown of the Blood-Brain Barrier. Am. J. Pathol. 172: 725-737 [Abstract] [Full Text]  
  • Boyer, J. D., Robinson, T. M., Kutzler, M. A., Vansant, G., Hokey, D. A., Kumar, S., Parkinson, R., Wu, L., Sidhu, M. K., Pavlakis, G. N., Felber, B. K., Brown, C., Silvera, P., Lewis, M. G., Monforte, J., Waldmann, T. A., Eldridge, J., Weiner, D. B. (2007). Protection against simian/human immunodeficiency virus (SHIV) 89.6P in macaques after coimmunization with SHIV antigen and IL-15 plasmid. Proc. Natl. Acad. Sci. USA 104: 18648-18653 [Abstract] [Full Text]  
  • Kuwata, T., Byrum, R., Whitted, S., Goeken, R., Buckler-White, A., Plishka, R., Iyengar, R., Hirsch, V. M. (2007). A Rapid Progressor-Specific Variant Clone of Simian Immunodeficiency Virus Replicates Efficiently In Vivo Only in the Absence of Immune Reponses. J. Virol. 81: 8891-8904 [Abstract] [Full Text]  
  • Apetrei, C., Gautam, R., Sumpter, B., Carter, A. C., Gaufin, T., Staprans, S. I., Else, J., Barnes, M., Cao, R. Jr., Garg, S., Milush, J. M., Sodora, D. L., Pandrea, I., Silvestri, G. (2007). Virus Subtype-Specific Features of Natural Simian Immunodeficiency Virus SIVsmm Infection in Sooty Mangabeys. J. Virol. 81: 7913-7923 [Abstract] [Full Text]  
  • Brown, C. R., Czapiga, M., Kabat, J., Dang, Q., Ourmanov, I., Nishimura, Y., Martin, M. A., Hirsch, V. M. (2007). Unique Pathology in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques Is Consistent with a Pathogenesis Distinct from That of Classical AIDS. J. Virol. 81: 5594-5606 [Abstract] [Full Text]  
  • Nishimura, Y., Igarashi, T., Buckler-White, A., Buckler, C., Imamichi, H., Goeken, R. M., Lee, W. R., Lafont, B. A. P., Byrum, R., Lane, H. C., Hirsch, V. M., Martin, M. A. (2007). Loss of Naive Cells Accompanies Memory CD4+ T-Cell Depletion during Long-Term Progression to AIDS in Simian Immunodeficiency Virus-Infected Macaques. J. Virol. 81: 893-902 [Abstract] [Full Text]  
  • VandeWoude, S., Apetrei, C. (2006). Going Wild: Lessons from Naturally Occurring T-Lymphotropic Lentiviruses. Clin. Microbiol. Rev. 19: 728-762 [Abstract] [Full Text]  
  • Kuwata, T., Dehghani, H., Brown, C. R., Plishka, R., Buckler-White, A., Igarashi, T., Mattapallil, J., Roederer, M., Hirsch, V. M. (2006). Infectious Molecular Clones from a Simian Immunodeficiency Virus-Infected Rapid-Progressor (RP) Macaque: Evidence of Differential Selection of RP-Specific Envelope Mutations In Vitro and In Vivo. J. Virol. 80: 1463-1475 [Abstract] [Full Text]  
  • Peyerl, F. W., Barouch, D. H., Bazick, H. S., Manuel, E., Letvin, N. L. (2005). Use of Molecular Beacons for Rapid, Real-Time, Quantitative Monitoring of Cytotoxic T-Lymphocyte Epitope Mutations in Simian Immunodeficiency Virus. J. Clin. Microbiol. 43: 4773-4779 [Abstract] [Full Text]  
  • Borda, J. T., Alvarez, X., Kondova, I., Aye, P., Simon, M. A., Desrosiers, R. C., Lackner, A. A. (2004). Cell Tropism of Simian Immunodeficiency Virus in Culture Is Not Predictive of in Vivo Tropism or Pathogenesis. Am. J. Pathol. 165: 2111-2122 [Abstract] [Full Text]  
  • Apetrei, C., Gormus, B., Pandrea, I., Metzger, M., ten Haaft, P., Martin, L. N., Bohm, R., Alvarez, X., Koopman, G., Murphey-Corb, M., Veazey, R. S., Lackner, A. A., Baskin, G., Heeney, J., Marx, P. A. (2004). Direct Inoculation of Simian Immunodeficiency Virus from Sooty Mangabeys in Black Mangabeys (Lophocebus aterrimus): First Evidence of AIDS in a Heterologous African Species and Different Pathologic Outcomes of Experimental Infection. J. Virol. 78: 11506-11518 [Abstract] [Full Text]  
  • Staprans, S. I., Barry, A. P., Silvestri, G., Safrit, J. T., Kozyr, N., Sumpter, B., Nguyen, H., McClure, H., Montefiori, D., Cohen, J. I., Feinberg, M. B. (2004). Enhanced SIV replication and accelerated progression to AIDS in macaques primed to mount a CD4 T cell response to the SIV envelope protein. Proc. Natl. Acad. Sci. USA 101: 13026-13031 [Abstract] [Full Text]  
  • Nishimura, Y., Igarashi, T., Donau, O. K., Buckler-White, A., Buckler, C., Lafont, B. A. P., Goeken, R. M., Goldstein, S., Hirsch, V. M., Martin, M. A. (2004). Highly pathogenic SHIVs and SIVs target different CD4+ T cell subsets in rhesus monkeys, explaining their divergent clinical courses. Proc. Natl. Acad. Sci. USA 101: 12324-12329 [Abstract] [Full Text]  
  • Sadjadpour, R., Theodore, T. S., Igarashi, T., Donau, O. K., Plishka, R. J., Buckler-White, A., Martin, M. A. (2004). Induction of Disease by a Molecularly Cloned Highly Pathogenic Simian Immunodeficiency Virus/Human Immunodeficiency Virus Chimera Is Multigenic. J. Virol. 78: 5513-5519 [Abstract] [Full Text]  
  • O'Neil, S. P., Suwyn, C., Anderson, D. C., Niedziela, G., Bradley, J., Novembre, F. J., Herndon, J. G., McClure, H. M. (2004). Correlation of Acute Humoral Response with Brain Virus Burden and Survival Time in Pig-Tailed Macaques Infected with the Neurovirulent Simian Immunodeficiency Virus SIVsmmFGb. Am. J. Pathol. 164: 1157-1172 [Abstract] [Full Text]  
  • Hirsch, V. M., Santra, S., Goldstein, S., Plishka, R., Buckler-White, A., Seth, A., Ourmanov, I., Brown, C. R., Engle, R., Montefiori, D., Glowczwskie, J., Kunstman, K., Wolinsky, S., Letvin, N. L. (2004). Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques. J. Virol. 78: 275-284 [Abstract] [Full Text]  
  • Dehghani, H., Puffer, B. A., Doms, R. W., Hirsch, V. M. (2003). Unique Pattern of Convergent Envelope Evolution in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques: Association with CD4-Independent Usage of CCR5. J. Virol. 77: 6405-6418 [Abstract] [Full Text]  
  • Williams, K., Schwartz, A., Corey, S., Orandle, M., Kennedy, W., Thompson, B., Alvarez, X., Brown, C., Gartner, S., Lackner, A. (2002). Proliferating Cellular Nuclear Antigen Expression as a Marker of Perivascular Macrophages in Simian Immunodeficiency Virus Encephalitis. Am. J. Pathol. 161: 575-585 [Abstract] [Full Text]  
  • Orandle, M. S., MacLean, A. G., Sasseville, V. G., Alvarez, X., Lackner, A. A. (2002). Enhanced Expression of Proinflammatory Cytokines in the Central Nervous System Is Associated with Neuroinvasion by Simian Immunodeficiency Virus and the Development of Encephalitis. J. Virol. 76: 5797-5802 [Abstract] [Full Text]  
  • Dehghani, H., Brown, C. R., Plishka, R., Buckler-White, A., Hirsch, V. M. (2002). The ITAM in Nef Influences Acute Pathogenesis of AIDS-Inducing Simian Immunodeficiency Viruses SIVsm and SIVagm without Altering Kinetics or Extent of Viremia. J. Virol. 76: 4379-4389 [Abstract] [Full Text]  
  • Holterman, L., Dubbes, R., Mullins, J., Learn, G., Niphuis, H., Koornstra, W., Koopman, G., Kuhn, E.-M., Wade-Evans, A., Rosenwirth, B., Haaijman, J., Heeney, J. (2001). Characteristics of a Pathogenic Molecular Clone of an End-Stage Serum-Derived Variant of Simian Immunodeficiency Virus (SIVF359). J. Virol. 75: 9328-9338 [Abstract] [Full Text]  
  • Orandle, M. S., Williams, K. C., MacLean, A. G., Westmoreland, S. V., Lackner, A. A. (2001). Macaques with Rapid Disease Progression and Simian Immunodeficiency Virus Encephalitis Have a Unique Cytokine Profile in Peripheral Lymphoid Tissues. J. Virol. 75: 4448-4452 [Abstract] [Full Text]  
  • Williams, K. C., Corey, S., Westmoreland, S. V., Pauley, D., Knight, H., deBakker, C., Alvarez, X., Lackner, A. A. (2001). Perivascular Macrophages Are the Primary Cell Type Productively Infected by Simian Immunodeficiency Virus in the Brains of Macaques: Implications for the Neuropathogenesis of AIDS. JEM 193: 905-916 [Abstract] [Full Text]  
  • Igarashi, T., Brown, C. R., Endo, Y., Buckler-White, A., Plishka, R., Bischofberger, N., Hirsch, V., Martin, M. A. (2000). Macrophage are the principal reservoir and sustain high virus loads in rhesus macaques after the depletion of CD4+ T cells by a highly pathogenic simian immunodeficiency virus/HIV type 1 chimera (SHIV): Implications for HIV-1 infections of humans. Proc. Natl. Acad. Sci. USA 10.1073/pnas.021551798v1 [Abstract] [Full Text]  
  • Goldstein, S., Brown, C. R., Dehghani, H., Lifson, J. D., Hirsch, V. M. (2000). Intrinsic Susceptibility of Rhesus Macaque Peripheral CD4+ T Cells to Simian Immunodeficiency Virus In Vitro Is Predictive of In Vivo Viral Replication. J. Virol. 74: 9388-9395 [Abstract] [Full Text]  
  • Edinger, A. L., Ahuja, M., Sung, T., Baxter, K. C., Haggarty, B., Doms, R. W., Hoxie, J. A. (2000). Characterization and Epitope Mapping of Neutralizing Monoclonal Antibodies Produced by Immunization with Oligomeric Simian Immunodeficiency Virus Envelope Protein. J. Virol. 74: 7922-7935 [Abstract] [Full Text]  
  • Glamann, J., Hirsch, V. M. (2000). Characterization of a Macaque Recombinant Monoclonal Antibody That Binds to a CD4-Induced Epitope and Neutralizes Simian Immunodeficiency Virus. J. Virol. 74: 7158-7163 [Abstract] [Full Text]  
  • Ourmanov, I., Brown, C. R., Moss, B., Carroll, M., Wyatt, L., Pletneva, L., Goldstein, S., Venzon, D., Hirsch, V. M. (2000). Comparative Efficacy of Recombinant Modified Vaccinia Virus Ankara Expressing Simian Immunodeficiency Virus (SIV) Gag-Pol and/or Env in Macaques Challenged with Pathogenic SIV. J. Virol. 74: 2740-2751 [Abstract] [Full Text]  
  • Ourmanov, I., Bilska, M., Hirsch, V. M., Montefiori, D. C. (2000). Recombinant Modified Vaccinia Virus Ankara Expressing the Surface gp120 of Simian Immunodeficiency Virus (SIV) Primes for a Rapid Neutralizing Antibody Response to SIV Infection in Macaques. J. Virol. 74: 2960-2965 [Abstract] [Full Text]  
  • Wyand, M. S., Manson, K., Montefiori, D. C., Lifson, J. D., Johnson, R. P., Desrosiers, R. C. (1999). Protection by Live, Attenuated Simian Immunodeficiency Virus against Heterologous Challenge. J. Virol. 73: 8356-8363 [Abstract] [Full Text]  
  • Hirsch, V. M., Dapolito, G., Hahn, A., Lifson, J., Montefiori, D., Brown, C. R., Goeken, R. (1998). Viral Genetic Evolution in Macaques Infected with Molecularly Cloned Simian Immunodeficiency Virus Correlates with the Extent of Persistent Viremia. J. Virol. 72: 6482-6489 [Abstract] [Full Text]  
  • Rudensey, L. M., Kimata, J. T., Long, E. M., Chackerian, B., Overbaugh, J. (1998). Changes in the Extracellular Envelope Glycoprotein of Variants That Evolve during the Course of Simian Immunodeficiency Virus SIVMne Infection Affect Neutralizing Antibody Recognition, Syncytium Formation, and Macrophage Tropism but Not Replication, Cytopathicity, or CCR-5 Coreceptor Recognition. J. Virol. 72: 209-217 [Abstract] [Full Text]  
  • Glamann, J., Burton, D. R., Parren, P. W. H. I., Ditzel, H. J., Kent, K. A., Arnold, C., Montefiori, D., Hirsch, V. M. (1998). Simian Immunodeficiency Virus (SIV) Envelope-Specific Fabs with High-Level Homologous Neutralizing Activity: Recovery from a Long-Term-Nonprogressor SIV-Infected Macaque. J. Virol. 72: 585-592 [Abstract] [Full Text]  
  • Igarashi, T., Brown, C. R., Endo, Y., Buckler-White, A., Plishka, R., Bischofberger, N., Hirsch, V., Martin, M. A. (2001). Macrophage are the principal reservoir and sustain high virus loads in rhesus macaques after the depletion of CD4+ T cells by a highly pathogenic simian immunodeficiency virus/HIV type 1 chimera (SHIV): Implications for HIV-1 infections of humans. Proc. Natl. Acad. Sci. USA 98: 658-663 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»