This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Davey, R. A. Articles by Cunningham, J. M. Search for Related Content PubMed PubMed Citation Articles by Davey, R. A. Articles by Cunningham, J. M.

 Previous Article  |  Next Article 

J. Virol., 11 1997, 8096-8102, Vol 71, No. 11
Copyright © 1997, American Society for Microbiology

In vitro binding of purified murine ecotropic retrovirus envelope surface protein to its receptor, MCAT-1

RA Davey, CA Hamson, JJ Healey and JM Cunningham
Howard Hughes Medical Institute, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.

An amino-terminal portion of the Friend murine leukemia virus (MLV) envelope surface protein [SU, residues 1 to 236 [SU:(1-236)]] and its receptor, MCAT-1, were each purified from insect cells after expression by using recombinant baculoviruses. Friend SU:(1-236) bound specifically to Xenopus oocytes that expressed MCAT-1 with an affinity (Kd, 55 nM) similar to that of viral SU binding to permissive cells. Direct binding of Friend SU:(1-236) to purified MCAT-1 was observed in detergent and after reconstitution into liposomes. Analysis of binding demonstrated that MCAT-1 and Friend SU:(1-236) interact with a stoichiometry of near 1:1. These findings demonstrate that the amino- terminal domain from the SU of ecotropic murine retroviruses contains an MCAT-1 binding domain.

This article has been cited by other articles:

• Kumar, P., Nachagari, D., Fields, C., Franks, J., Albritton, L. M. (2007). Host Cell Cathepsins Potentiate Moloney Murine Leukemia Virus Infection. J. Virol. 81: 10506-10514 [Abstract] [Full Text]  
• Duda, A., Luftenegger, D., Pietschmann, T., Lindemann, D. (2006). Characterization of the prototype foamy virus envelope glycoprotein receptor-binding domain.. J. Virol. 80: 8158-8167 [Abstract] [Full Text]  
• Murphy, S. L., Chung-Landers, M., Honczarenko, M., Gaulton, G. N. (2006). Linkage of Reduced Receptor Affinity and Superinfection to Pathogenesis of TR1.3 Murine Leukemia Virus. J. Virol. 80: 4601-4609 [Abstract] [Full Text]  
• Lucas, M. L., Seidel, N. E., Porada, C. D., Quigley, J. G., Anderson, S. M., Malech, H. L., Abkowitz, J. L., Zanjani, E. D., Bodine, D. M. (2005). Improved transduction of human sheep repopulating cells by retrovirus vectors pseudotyped with feline leukemia virus type C or RD114 envelopes. Blood 106: 51-58 [Abstract] [Full Text]  
• Burkhart, M. D., D'Agostino, P., Kayman, S. C., Pinter, A. (2005). Involvement of the C-Terminal Disulfide-Bonded Loop of Murine Leukemia Virus SU Protein in a Postbinding Step Critical for Viral Entry. J. Virol. 79: 7868-7876 [Abstract] [Full Text]  
• Forster, F., Medalia, O., Zauberman, N., Baumeister, W., Fass, D. (2005). Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography. Proc. Natl. Acad. Sci. USA 102: 4729-4734 [Abstract] [Full Text]  
• Vigdorovich, V., Strong, R. K., Miller, A. D. (2005). Expression and Characterization of a Soluble, Active Form of the Jaagsiekte Sheep Retrovirus Receptor, Hyal2. J. Virol. 79: 79-86 [Abstract] [Full Text]  
• Bao, X., Reuss, L., Altenberg, G. A. (2004). Regulation of Purified and Reconstituted Connexin 43 Hemichannels by Protein Kinase C-mediated Phosphorylation of Serine 368. J. Biol. Chem. 279: 20058-20066 [Abstract] [Full Text]  
• Qian, Z., Albritton, L. M. (2004). An Aromatic Side Chain Is Required at Residue 8 of SU for Fusion of Ecotropic Murine Leukemia Virus. J. Virol. 78: 508-512 [Abstract] [Full Text]  
• Zhang, Y., Rassa, J. C., deObaldia, M. E., Albritton, L. M., Ross, S. R. (2003). Identification of the Receptor Binding Domain of the Mouse Mammary Tumor Virus Envelope Protein. J. Virol. 77: 10468-10478 [Abstract] [Full Text]  
• Chow, Y.-H. J., Alberti, A., Mura, M., Pretto, C., Murcia, P., Albritton, L. M., Palmarini, M. (2003). Transformation of Rodent Fibroblasts by the Jaagsiekte Sheep Retrovirus Envelope Is Receptor Independent and Does Not Require the Surface Domain. J. Virol. 77: 6341-6350 [Abstract] [Full Text]  
• Burkhart, M. D., Kayman, S. C., He, Y., Pinter, A. (2003). Distinct Mechanisms of Neutralization by Monoclonal Antibodies Specific for Sites in the N-Terminal or C-Terminal Domain of Murine Leukemia Virus SU. J. Virol. 77: 3993-4003 [Abstract] [Full Text]  
• Seelenmeyer, C., Wegehingel, S., Lechner, J., Nickel, W. (2003). The cancer antigen CA125 represents a novel counter receptor for galectin-1. J. Cell Sci. 116: 1305-1318 [Abstract] [Full Text]  
• Wensel, D. L., Li, W., Cunningham, J. M. (2003). A Virus-Virus Interaction Circumvents the Virus Receptor Requirement for Infection by Pathogenic Retroviruses. J. Virol. 77: 3460-3469 [Abstract] [Full Text]  
• Barnett, A. L., Wensel, D. L., Li, W., Fass, D., Cunningham, J. M. (2003). Structure and Mechanism of a Coreceptor for Infection by a Pathogenic Feline Retrovirus. J. Virol. 77: 2717-2729 [Abstract] [Full Text]  
• Kizhatil, K., Albritton, L. M. (2002). System y+ localizes to different membrane subdomains in the basolateral plasma membrane of epithelial cells. Am. J. Physiol. Cell Physiol. 283: C1784-C1794 [Abstract] [Full Text]  
• Engling, A., Backhaus, R., Stegmayer, C., Zehe, C., Seelenmeyer, C., Kehlenbach, A., Schwappach, B., Wegehingel, S., Nickel, W. (2002). Biosynthetic FGF-2 is targeted to non-lipid raft microdomains following translocation to the extracellular surface of CHO cells. J. Cell Sci. 115: 3619-3631 [Abstract] [Full Text]  
• Lauring, A. S., Cheng, H. H., Eiden, M. V., Overbaugh, J. (2002). Genetic and Biochemical Analyses of Receptor and Cofactor Determinants for T-Cell-Tropic Feline Leukemia Virus Infection. J. Virol. 76: 8069-8078 [Abstract] [Full Text]  
• Barnett, A. L., Cunningham, J. M. (2001). Receptor Binding Transforms the Surface Subunit of the Mammalian C-Type Retrovirus Envelope Protein from an Inhibitor to an Activator of Fusion. J. Virol. 75: 9096-9105 [Abstract] [Full Text]  
• Overbaugh, J., Miller, A. D., Eiden, M. V. (2001). Receptors and Entry Cofactors for Retroviruses Include Single and Multiple Transmembrane-Spanning Proteins as well as Newly Described Glycophosphatidylinositol-Anchored and Secreted Proteins. Microbiol. Mol. Biol. Rev. 65: 371-389 [Abstract] [Full Text]  
• Sugai, J., Eiden, M., Anderson, M. M., Van Hoeven, N., Meiering, C. D., Overbaugh, J. (2001). Identification of Envelope Determinants of Feline Leukemia Virus Subgroup B That Permit Infection and Gene Transfer to Cells Expressing Human Pit1 or Pit2. J. Virol. 75: 6841-6849 [Abstract] [Full Text]  
• Lu, C.-W., Roth, M. J. (2001). Functional Characterization of the N Termini of Murine Leukemia Virus Envelope Proteins. J. Virol. 75: 4357-4366 [Abstract] [Full Text]  
• Barnett, A. L., Davey, R. A., Cunningham, J. M. (2001). Modular organization of the Friend murine leukemia virus envelope protein underlies the mechanism of infection. Proc. Natl. Acad. Sci. USA 98: 4113-4118 [Abstract] [Full Text]  
• Kim, S. Y., Ferrell, J. E. Jr., Chae, S.-K., Lee, K.-J. (2000). Inhibition of Progesterone-induced Xenopus Oocyte Maturation by Nm23. Cell Growth Differ. 11: 485-490 [Abstract] [Full Text]  
• Gwynn, S. R., Hankenson, F. C., Lauring, A. S., Rohn, J. L., Overbaugh, J. (2000). Feline Leukemia Virus Envelope Sequences That Affect T-Cell Tropism and Syncytium Formation Are Not Part of Known Receptor-Binding Domains. J. Virol. 74: 5754-5761 [Abstract] [Full Text]  
• O'Reilly, L., Roth, M. J. (2000). Second-Site Changes Affect Viability of Amphotropic/Ecotropic Chimeric Enveloped Murine Leukemia Viruses. J. Virol. 74: 899-913 [Abstract] [Full Text]  
• Masuda, M., Kakushima, N., Wilt, S. G., Ruscetti, S. K., Hoffman, P. M., Iwamoto, A., Masuda, M. (1999). Analysis of Receptor Usage by Ecotropic Murine Retroviruses, Using Green Fluorescent Protein-Tagged Cationic Amino Acid Transporters. J. Virol. 73: 8623-8629 [Abstract] [Full Text]  
• Davey, R. A., Zuo, Y., Cunningham, J. M. (1999). Identification of a Receptor-Binding Pocket on the Envelope Protein of Friend Murine Leukemia Virus. J. Virol. 73: 3758-3763 [Abstract] [Full Text]  
• Miyagi, T., Chuang, L. F., Doi, R. H., Carlos, M. P., Torres, J. V., Chuang, R. Y. (2000). Morphine Induces Gene Expression of CCR5 in Human CEM x174 Lymphocytes. J. Biol. Chem. 275: 31305-31310 [Abstract] [Full Text]