This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huber, M. Articles by Kandolf, R. Search for Related Content PubMed PubMed Citation Articles by Huber, M. Articles by Kandolf, R.

 Previous Article  |  Next Article 

J. Virol., 01 1997, 595-600, Vol 71, No. 1
Copyright © 1997, American Society for Microbiology

Tyrosine phosphorylation events during coxsackievirus B3 replication

M Huber, HC Selinka and R Kandolf
Institute for Pathology, Department of Molecular Pathology, University of Tubingen, Germany.

In order to study cellular and viral determinants of pathogenicity, interactions between coxsackievirus B3 (CVB3) replication and cellular protein tyrosine phosphorylation were investigated. During CVB3 infection of HeLa cells, distinct proteins become phosphorylated on tyrosine residues, as detected by the use of antiphosphotyrosine Western blotting. Two proteins of 48 and 200 kDa showed enhanced tyrosine phosphorylation 4 to 5 h postinfection (p.i.), although virus- induced inhibition of cellular protein synthesis had already occurred 3 to 4 h p.i. Subcellular fractionation experiments revealed distinct localization of tyrosine-phosphorylated proteins of 48 and 200 kDa in the cytosol and membrane fractions of infected cells, respectively. In addition, in Vero cells infected with CVB3, echovirus (EV)11, or EV12, increased tyrosine phosphorylation of a 200-kDa protein was detected 6 h p.i. Herbimycin A, a specific inhibitor of Src-like protein tyrosine kinases, was shown to inhibit virus-induced tyrosine phosphorylations and to reduce the production of progeny virions. In contrast, in cells treated with the inhibitors staurosporine and calphostin C, the synthesis of progeny virions was not affected. Immunoprecipitation experiments suggested that the tyrosine-phosphorylated 200-kDa protein in CVB3-infected cells is of cellular origin. In summary, these investigations have begun to unravel the effect of CVB3 as well as EV11 and EV12 replication on cellular tyrosine phosphorylation and support the importance of tyrosine phosphorylation events for effective virus replication. Such cellular phosphorylation events triggered in the course of enterovirus infection may enhance virus replication.

This article has been cited by other articles:

• Hirasawa, K., Kim, A., Han, H.-S., Han, J., Jun, H.-S., Yoon, J.-W. (2003). Effect of p38 Mitogen-Activated Protein Kinase on the Replication of Encephalomyocarditis Virus. J. Virol. 77: 5649-5656 [Abstract] [Full Text]  
• Whiteman, S. C., Bianco, A., Knight, R. A., Spiteri, M. A. (2003). Human Rhinovirus Selectively Modulates Membranous and Soluble Forms of Its Intercellular Adhesion Molecule-1 (ICAM-1) Receptor to Promote Epithelial Cell Infectivity. J. Biol. Chem. 278: 11954-11961 [Abstract] [Full Text]  
• Klingel, K., Selinka, H.-C., Sauter, M., Bock, C.-T., Szalay, G., Kandolf, R. (2002). Molecular mechanisms in enterovirus and parvovirus B19 associated myocarditis and inflammatory cardiomyopathy. Eur Heart J Suppl 4: I8-I12 [Abstract]  
• Peng, T., Sadusky, T., Li, Y., Coulton, G. R., Zhang, H., Archard, L. C. (2001). Altered expression of Bag-1 in Coxsackievirus B3 infected mouse heart. Cardiovasc Res 50: 46-55 [Abstract] [Full Text]  
• Henke, A., Launhardt, H., Klement, K., Stelzner, A., Zell, R., Munder, T. (2000). Apoptosis in Coxsackievirus B3-Caused Diseases: Interaction between the Capsid Protein VP2 and the Proapoptotic Protein Siva. J. Virol. 74: 4284-4290 [Abstract] [Full Text]  
• Huber, M., Watson, K. A., Selinka, H.-C., Carthy, C. M., Klingel, K., McManus, B. M., Kandolf, R. (1999). Cleavage of RasGAP and Phosphorylation of Mitogen-Activated Protein Kinase in the Course of Coxsackievirus B3 Replication. J. Virol. 73: 3587-3594 [Abstract] [Full Text]  
• Carthy, C. M., Granville, D. J., Watson, K. A., Anderson, D. R., Wilson, J. E., Yang, D., Hunt, D. W. C., McManus, B. M. (1998). Caspase Activation and Specific Cleavage of Substrates after Coxsackievirus B3-Induced Cytopathic Effect in HeLa Cells. J. Virol. 72: 7669-7675 [Abstract] [Full Text]