This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martinez-Costas, J. Articles by Benavente, J. Search for Related Content PubMed PubMed Citation Articles by Martinez-Costas, J. Articles by Benavente, J.

 Previous Article  |  Next Article 

J. Virol., Jan 1997, 59-64, Vol 71, No. 1
Copyright © 1997, American Society for Microbiology

Protein architecture of avian reovirus S1133 and identification of the cell attachment protein

J Martinez-Costas, A Grande, R Varela, C Garcia-Martinez and J Benavente
Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia,University of Santiago, Spain.

There are a number of discrepancies in the literature regarding the protein composition of the avian reoviruses. The present study demonstrates that avian reovirus S1133 contains at least 10 proteins (lambdaA, lambdaB, lambdaC, muA, muB, muBC, muBN, sigmaA, sigmaB, and sigmaC). Polypeptides muB, muBC, muBN, sigmaB, and sigmaC are components of the outer capsid layer of the virus, while lambdaA, lambdaB, muA, and sigmaA are core polypeptides. Protein lambdaC is a component of both layers, extending from the inner core to the outer capsid. The minor outer-capsid polypeptide sigmaC is shown to be the cell attachment protein, since it is the only viral polypeptide present in extracts of S1133-infected cells that binds specifically to chicken embryo fibroblasts; furthermore, its binding to avian cells was competitively inhibited by S1133 reovirions but not by mammalian reovirions. Our results also show that sigmaC is an oligomeric protein both in the virion and free in the cytoplasm, and preliminary results suggest that the multimer is made up of three monomeric units.

This article has been cited by other articles:

• Costas, C., Martinez-Costas, J., Bodelon, G., Benavente, J. (2005). The Second Open Reading Frame of the Avian Reovirus S1 Gene Encodes a Transcription-Dependent and CRM1-Independent Nucleocytoplasmic Shuttling Protein. J. Virol. 79: 2141-2150 [Abstract] [Full Text]  
• Xu, W., Patrick, M. K., Hazelton, P. R., Coombs, K. M. (2004). Avian Reovirus Temperature-Sensitive Mutant tsA12 Has a Lesion in Major Core Protein {sigma}A and Is Defective in Assembly. J. Virol. 78: 11142-11151 [Abstract] [Full Text]  
• Gonzalez-Lopez, C., Martinez-Costas, J., Esteban, M., Benavente, J. (2003). Evidence that avian reovirus {sigma}A protein is an inhibitor of the double-stranded RNA-dependent protein kinase. J. Gen. Virol. 84: 1629-1639 [Abstract] [Full Text]  
• Bodelon, G., Labrada, L., Martinez-Costas, J., Benavente, J. (2002). Modification of Late Membrane Permeability in Avian Reovirus-infected Cells. VIROPORIN ACTIVITY OF THE S1-ENCODED NONSTRUCTURAL p10 PROTEIN. J. Biol. Chem. 277: 17789-17796 [Abstract] [Full Text]  
• Shmulevitz, M., Yameen, Z., Dawe, S., Shou, J., O'Hara, D., Holmes, I., Duncan, R. (2002). Sequential Partially Overlapping Gene Arrangement in the Tricistronic S1 Genome Segments of Avian Reovirus and Nelson Bay Reovirus: Implications for Translation Initiation. J. Virol. 76: 609-618 [Abstract] [Full Text]  
• Grande, A., Costas, C., Benavente, J. (2002). Subunit composition and conformational stability of the oligomeric form of the avian reovirus cell-attachment protein {sigma}C. J. Gen. Virol. 83: 131-139 [Abstract] [Full Text]  
• O'Hara, D., Patrick, M., Cepica, D., Coombs, K. M., Duncan, R. (2001). Avian Reovirus Major {micro}-Class Outer Capsid Protein Influences Efficiency of Productive Macrophage Infection in a Virus Strain-Specific Manner. J. Virol. 75: 5027-5035 [Abstract] [Full Text]  
• Le Gall-Recule, G, Cherbonnel, M, Arnauld, C, Blanchard, P, Jestin, A, Jestin, V (1999). Molecular characterization and expression of the S3 gene of muscovy duck reovirus strain 89026. J. Gen. Virol. 80: 195-203 [Abstract]