This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zaiman, A. L.
Right arrow Articles by Lenz, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zaiman, A. L.
Right arrow Articles by Lenz, J.

 Previous Article  |  Next Article 

J. Virol., Aug 1996, 5618-5629, Vol 70, No. 8
Copyright © 1996, American Society for Microbiology

Transcriptional activation of a retrovirus enhancer by CBF (AML1) requires a second factor: evidence for cooperativity with c-Myb

AL Zaiman and J Lenz
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Transcriptional enhancer sequences within the long terminal repeats (LTRs) of murine leukemia viruses are the primary genetic determinants of the tissue specificity and potency of the oncogenic potential of these retroviruses. SL3-3 (SL3) is a murine leukemia virus that induces T-cell lymphomas. The LTR enhancer of this virus contains two binding sites for the transcription factor CBF (also called AML1 and PEBP2) that flank binding sites for c-Myb and the Ets family of factors. Using cotransfection assays in P19 cells, we report here that CBF and c-Myb cooperatively stimulate transcription from the SL3 LTR. By itself, c- Myb had no stimulatory effect on transcription. However, when cotransfected with a cDNA encoding one form of the alpha subunit of CBF called CBFalpha2-451, a level of transactivation higher than that seen with CBFalpha2-451 alone was detected. The negative regulatory domain near the carboxyl terminus of c-Myb did not affect this activity. Electrophoretic mobility shift assays indicated that CBF and c-Myb bind to DNA independently. Therefore, it appears that the cooperative stimulation of transcription by these factors occurs at a step in the process of transcription after the two factors are bound to the enhancer. Sequences near the carboxyl terminus of CBFalpha2-451 were important for cooperativity with c-Myb, consistent with previous reports that this region contains an activation domain. However, CBFalpha2-451 failed to activate transcription from a version of the SL3 LTR in which the enhancer was replaced with five tandem CBF-binding sites. Thus, it appears that transcriptional activation of the SL3 enhancer by CBF requires that an appropriate heterologous transcription factor be bound to a neighboring site in the regulatory sequences.


This article has been cited by other articles:

  • Tani-ichi, S., Satake, M., Ikuta, K. (2009). Activation of the mouse TCR{gamma} enhancers by STAT5. Int Immunol 21: 1079-1088 [Abstract] [Full Text]  
  • Ejegod, D., Sorensen, K. D., Mossbrugger, I., Quintanilla-Martinez, L., Schmidt, J., Pedersen, F. S. (2009). Control of Pathogenicity and Disease Specificity of a T-Lymphomagenic Gammaretrovirus by E-Box Motifs but Not by an Overlapping Glucocorticoid Response Element. J. Virol. 83: 336-346 [Abstract] [Full Text]  
  • Marshall, L. J., Moore, A. C., Ohki, M., Kitabayashi, I., Patterson, D., Ornelles, D. A. (2008). RUNX1 Permits E4orf6-Directed Nuclear Localization of the Adenovirus E1B-55K Protein and Associates with Centers of Viral DNA and RNA Synthesis. J. Virol. 82: 6395-6408 [Abstract] [Full Text]  
  • Sorensen, K. D., Quintanilla-Martinez, L., Kunder, S., Schmidt, J., Pedersen, F. S. (2004). Mutation of All Runx (AML1/Core) Sites in the Enhancer of T-Lymphomagenic SL3-3 Murine Leukemia Virus Unmasks a Significant Potential for Myeloid Leukemia Induction and Favors Enhancer Evolution toward Induction of Other Disease Patterns. J. Virol. 78: 13216-13231 [Abstract] [Full Text]  
  • DiFronzo, N. L., Frieder, M., Loiler, S. A., Pham, Q. N., Holland, C. A. (2003). Duplication of U3 Sequences in the Long Terminal Repeat of Mink Cell Focus-Inducing Viruses Generates Redundancies of Transcription Factor Binding Sites Important for the Induction of Thymomas. J. Virol. 77: 3326-3333 [Abstract] [Full Text]  
  • Hernandez-Munain, C., Krangel, M. S. (2002). Distinct Roles for c-Myb and Core Binding Factor/Polyoma Enhancer-Binding Protein 2 in the Assembly and Function of a Multiprotein Complex on the TCR {delta} Enhancer In Vivo. J. Immunol. 169: 4362-4369 [Abstract] [Full Text]  
  • Allen, R. D. III, Kim, H. K., Sarafova, S. D., Siu, G. (2001). Negative Regulation of CD4 Gene Expression by a HES-1-c-Myb Complex. Mol. Cell. Biol. 21: 3071-3082 [Abstract] [Full Text]  
  • Zhang, J., Hug, B. A., Huang, E. Y., Chen, C. W., Gelmetti, V., Maccarana, M., Minucci, S., Pelicci, P. G., Lazar, M. A. (2001). Oligomerization of ETO Is Obligatory for Corepressor Interaction. Mol. Cell. Biol. 21: 156-163 [Abstract] [Full Text]  
  • Javed, A, Guo, B, Hiebert, S, Choi, J., Green, J, Zhao, S., Osborne, M., Stifani, S, Stein, J., Lian, J., van Wijnen, A., Stein, G. (2000). Groucho/TLE/R-esp proteins associate with the nuclear matrix and repress RUNX (CBF(alpha)/AML/PEBP2(alpha)) dependent activation of tissue-specific gene transcription. J. Cell Sci. 113: 2221-2231 [Abstract]  
  • Ethelberg, S., Tzschaschel, B. D., Luz, A., Diaz-Cano, S. J., Pedersen, F. S., Schmidt, J. (1999). Increased Induction of Osteopetrosis, but Unaltered Lymphomagenicity, by Murine Leukemia Virus SL3-3 after Mutation of a Nuclear Factor 1 Site in the Enhancer. J. Virol. 73: 10406-10415 [Abstract] [Full Text]  
  • Martiney, M. J., Levy, L. S., Lenz, J. (1999). Suppressor Mutations within the Core Binding Factor (CBF/AML1) Binding Site of a T-Cell Lymphomagenic Retrovirus. J. Virol. 73: 2143-2152 [Abstract] [Full Text]  
  • DiFronzo, N. L., Holland, C. A. (1999). Sequence-Specific and/or Stereospecific Constraints of the U3 Enhancer Elements of MCF 247-W Are Important for Pathogenicity. J. Virol. 73: 234-241 [Abstract] [Full Text]  
  • Levanon, D., Goldstein, R. E., Bernstein, Y., Tang, H., Goldenberg, D., Stifani, S., Paroush, Z.'e., Groner, Y. (1998). Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors. Proc. Natl. Acad. Sci. USA 95: 11590-11595 [Abstract] [Full Text]  
  • Barat, C., Rassart, E. (1998). Members of the GATA Family of Transcription Factors Bind to the U3 Region of Cas-Br-E and Graffi Retroviruses and Transactivate Their Expression. J. Virol. 72: 5579-5588 [Abstract] [Full Text]  
  • Zaiman, A. L., Nieves, A., Lenz, J. (1998). CBF, Myb, and Ets Binding Sites Are Important for Activity of the Core I Element of the Murine Retrovirus SL3-3 in T Lymphocytes. J. Virol. 72: 3129-3137 [Abstract] [Full Text]  
  • Stewart, M., Terry, A., Hu, M., O'Hara, M., Blyth, K., Baxter, E., Cameron, E., Onions, D. E., Neil, J. C. (1997). Proviral insertions induce the expression of bone-specific isoforms of PEBP2alpha A (CBFA1): Evidence for a new myc collaborating oncogene. Proc. Natl. Acad. Sci. USA 94: 8646-8651 [Abstract] [Full Text]  
  • Coffman, J., Kirchhamer, C., Harrington, M., Davidson, E. (1997). SpMyb functions as an intramodular repressor to regulate spatial expression of CyIIIa in sea urchin embryos. Development 124: 4717-4727 [Abstract]  
  • McCarthy, T. L., Ji, C., Chen, Y., Kim, K. K., Imagawa, M., Ito, Y., Centrella, M. (2000). Runt Domain Factor (Runx)-dependent Effects on CCAAT/ Enhancer-binding Protein delta Expression and Activity in Osteoblasts. J. Biol. Chem. 275: 21746-21753 [Abstract] [Full Text]  
  • Gutierrez, S., Javed, A., Tennant, D. K., van Rees, M., Montecino, M., Stein, G. S., Stein, J. L., Lian, J. B. (2002). CCAAT/Enhancer-binding Proteins (C/EBP) beta and delta Activate Osteocalcin Gene Transcription and Synergize with Runx2 at the C/EBP Element to Regulate Bone-specific Expression. J. Biol. Chem. 277: 1316-1323 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»