This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zolotukhin, S.
Right arrow Articles by Muzyczka, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zolotukhin, S.
Right arrow Articles by Muzyczka, N.

 Previous Article  |  Next Article 

J. Virol., Jul 1996, 4646-4654, Vol 70, No. 7
Copyright © 1996, American Society for Microbiology

A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells

S Zolotukhin, M Potter, WW Hauswirth, J Guy and N Muzyczka
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida 32610, USA.

We constructed gfph, a synthetic version of the jellyfish Aequorea victoria green fluorescent protein (gfp) cDNA that is adapted for high- level expression in mammalian cells, especially those of human origin. A total of 92 base substitutions were made in 88 codons in order to change the codon usage within the gfp10 coding sequence so that it was more appropriate for expression in mammalian cells. We also describe a series of versatile recombinant adeno-associated virus and adenovirus vectors for delivery and expression of genes into mammalian cells and, using these vectors, demonstrate the efficient transduction and expression of the gfph gene in the human cell line 293 and also in vivo, within neurosensory cells of guinea pig eye. Cells infected with recombinant adeno-associated virus-GFPH can be readily sorted by fluorescence-activated cell sorting, suggesting that the newly designed gfph gene could be widely used as a reporter in many gene delivery technologies, including human gene therapy.


This article has been cited by other articles:

  • Glenn, S. T., Jones, C. A., Pan, L., Gross, K. W. (2008). In vivo analysis of key elements within the renin regulatory region. Physiol. Genomics 35: 243-253 [Abstract] [Full Text]  
  • Rodino-Klapac, L. R., Lee, J-S, Mulligan, R. C., Clark, K. R., Mendell, J. R. (2008). Lack of toxicity of alpha-sarcoglycan overexpression supports clinical gene transfer trial in LGMD2D. Neurology 71: 240-247 [Abstract] [Full Text]  
  • Grimm, D., Lee, J. S., Wang, L., Desai, T., Akache, B., Storm, T. A., Kay, M. A. (2008). In Vitro and In Vivo Gene Therapy Vector Evolution via Multispecies Interbreeding and Retargeting of Adeno-Associated Viruses. J. Virol. 82: 5887-5911 [Abstract] [Full Text]  
  • Thones, N., Herreiner, A., Schadlich, L., Piuko, K., Muller, M. (2008). A Direct Comparison of Human Papillomavirus Type 16 L1 Particles Reveals a Lower Immunogenicity of Capsomeres than Viruslike Particles with Respect to the Induced Antibody Response. J. Virol. 82: 5472-5485 [Abstract] [Full Text]  
  • Winnard, P. T. Jr., Pathak, A. P., Dhara, S., Cho, S. Y., Raman, V., Pomper, M. G. (2008). Molecular Imaging of Metastatic Potential. JNM 49: 96S-112S [Abstract] [Full Text]  
  • Carson, S. D., Kim, K.-S., Pirruccello, S. J., Tracy, S., Chapman, N. M. (2007). Endogenous low-level expression of the coxsackievirus and adenovirus receptor enables coxsackievirus B3 infection of RD cells. J. Gen. Virol. 88: 3031-3038 [Abstract] [Full Text]  
  • Mouri, A., Noda, Y., Hara, H., Mizoguchi, H., Tabira, T., Nabeshima, T. (2007). Oral vaccination with a viral vector containing A{beta} cDNA attenuates age-related A{beta} accumulation and memory deficits without causing inflammation in a mouse Alzheimer model. FASEB J. 21: 2135-2148 [Abstract] [Full Text]  
  • Murphy, M., Gomos-Klein, J., Stankic, M., Falck-Pedersen, E. (2007). Adeno-Associated Virus Type 2 p5 Promoter: a Rep-Regulated DNA Switch Element Functioning in Transcription, Replication, and Site-Specific Integration. J. Virol. 81: 3721-3730 [Abstract] [Full Text]  
  • Eslamboli, A., Romero-Ramos, M., Burger, C., Bjorklund, T., Muzyczka, N., Mandel, R. J., Baker, H., Ridley, R. M., Kirik, D. (2007). Long-term consequences of human alpha-synuclein overexpression in the primate ventral midbrain. Brain 130: 799-815 [Abstract] [Full Text]  
  • Yu, C.-Y., Hsu, Y.-W., Liao, C.-L., Lin, Y.-L. (2006). Flavivirus Infection Activates the XBP1 Pathway of the Unfolded Protein Response To Cope with Endoplasmic Reticulum Stress. J. Virol. 80: 11868-11880 [Abstract] [Full Text]  
  • Huang, L.-R., Wu, H.-L., Chen, P.-J., Chen, D.-S. (2006). An immunocompetent mouse model for the tolerance of human chronic hepatitis B virus infection. Proc. Natl. Acad. Sci. USA 103: 17862-17867 [Abstract] [Full Text]  
  • Bona, R., Andreotti, M., Buffa, V., Leone, P., Galluzzo, C. M., Amici, R., Palmisano, L., Mancini, M. G., Michelini, Z., Di Santo, R., Costi, R., Roux, A., Pommier, Y., Marchand, C., Vella, S., Cara, A. (2006). Development of a human immunodeficiency virus vector-based, single-cycle assay for evaluation of anti-integrase compounds.. Antimicrob. Agents Chemother. 50: 3407-3417 [Abstract] [Full Text]  
  • Mueller, S., Papamichail, D., Coleman, J. R., Skiena, S., Wimmer, E. (2006). Reduction of the Rate of Poliovirus Protein Synthesis through Large-Scale Codon Deoptimization Causes Attenuation of Viral Virulence by Lowering Specific Infectivity. J. Virol. 80: 9687-9696 [Abstract] [Full Text]  
  • Press, W. H., Robins, H. (2006). Isochores Exhibit Evidence of Genes Interacting With the Large-Scale Genomic Environment. Genetics 174: 1029-1040 [Abstract] [Full Text]  
  • Glushakova, L. G., Timmers, A. M., Pang, J., Teusner, J. T., Hauswirth, W. W. (2006). Human blue-opsin promoter preferentially targets reporter gene expression to rat s-cone photoreceptors.. IOVS 47: 3505-3513 [Abstract] [Full Text]  
  • Buffa, V., Negri, D. R. M., Leone, P., Bona, R., Borghi, M., Bacigalupo, I., Carlei, D., Sgadari, C., Ensoli, B., Cara, A. (2006). A single administration of lentiviral vectors expressing either full-length human immunodeficiency virus 1 (HIV-1)HXB2 Rev/Env or codon-optimized HIV-1JR-FL gp120 generates durable immune responses in mice. J. Gen. Virol. 87: 1625-1634 [Abstract] [Full Text]  
  • Pernet, V., Di Polo, A. (2006). Synergistic action of brain-derived neurotrophic factor and lens injury promotes retinal ganglion cell survival, but leads to optic nerve dystrophy in vivo. Brain 129: 1014-1026 [Abstract] [Full Text]  
  • Muller, O. J., Leuchs, B., Pleger, S. T., Grimm, D., Franz, W.-M., Katus, H. A., Kleinschmidt, J. A. (2006). Improved cardiac gene transfer by transcriptional and transductional targeting of adeno-associated viral vectors. Cardiovasc Res 70: 70-78 [Abstract] [Full Text]  
  • Takahashi, N., Nakashima, H. (2006). Negative Correlation of G+C Content at Silent Substitution Sites Between Orthologous Human and Mouse Protein-Coding Sequences. DNA Res 13: 135-140 [Abstract] [Full Text]  
  • Zhao, K.-N., Gu, W., Fang, N. X., Saunders, N. A., Frazer, I. H. (2005). Gene Codon Composition Determines Differentiation-Dependent Expression of a Viral Capsid Gene in Keratinocytes In Vitro and In Vivo. Mol. Cell. Biol. 25: 8643-8655 [Abstract] [Full Text]  
  • Liu, J., Timmers, A. M., Lewin, A. S., Hauswirth, W. W. (2005). Ribozyme Knockdown of the {gamma}-Subunit of Rod cGMP Phosphodiesterase Alters the ERG and Retinal Morphology in Wild-Type Mice. IOVS 46: 3836-3844 [Abstract] [Full Text]  
  • Kanekiyo, M., Matsuo, K., Hamatake, M., Hamano, T., Ohsu, T., Matsumoto, S., Yamada, T., Yamazaki, S., Hasegawa, A., Yamamoto, N., Honda, M. (2005). Mycobacterial Codon Optimization Enhances Antigen Expression and Virus-Specific Immune Responses in Recombinant Mycobacterium bovis Bacille Calmette-Guerin Expressing Human Immunodeficiency Virus Type 1 Gag. J. Virol. 79: 8716-8723 [Abstract] [Full Text]  
  • Hambsch, B., Grinevich, V., Seeburg, P. H., Schwarz, M. K. (2005). {gamma}-Protocadherins, Presenilin-mediated Release of C-terminal Fragment Promotes Locus Expression. J. Biol. Chem. 280: 15888-15897 [Abstract] [Full Text]  
  • Kickhoefer, V. A., Garcia, Y., Mikyas, Y., Johansson, E., Zhou, J. C., Raval-Fernandes, S., Minoofar, P., Zink, J. I., Dunn, B., Stewart, P. L., Rome, L. H. (2005). Engineering of vault nanocapsules with enzymatic and fluorescent properties. Proc. Natl. Acad. Sci. USA 102: 4348-4352 [Abstract] [Full Text]  
  • Hammond, T. M., Keller, N. P. (2005). RNA Silencing in Aspergillus nidulans Is Independent of RNA-Dependent RNA Polymerases. Genetics 169: 607-617 [Abstract] [Full Text]  
  • Paris, S., Sesboue, R. (2004). Metastasis models: the green fluorescent revolution?. Carcinogenesis 25: 2285-2292 [Abstract] [Full Text]  
  • Kang, S.-H. L., Levings, P. P, Andersen, F., Laipis, P. J, Berns, K. I, Zori, R. T, Bungert, J. (2004). Locus control region elements HS2 and HS3 in combination with chromatin boundaries confer high-level expression of a human {beta}-globin transgene in a centromeric region. GENES CELLS 9: 1043-1053 [Abstract] [Full Text]  
  • Becker, M. N., Greenleaf, W. B., Ostrov, D. A., Moyer, R. W. (2004). Amsacta moorei Entomopoxvirus Expresses an Active Superoxide Dismutase. J. Virol. 78: 10265-10275 [Abstract] [Full Text]  
  • Plotkin, J. B., Robins, H., Levine, A. J. (2004). Tissue-specific codon usage and the expression of human genes. Proc. Natl. Acad. Sci. USA 101: 12588-12591 [Abstract] [Full Text]  
  • Warrington, K. H. Jr., Gorbatyuk, O. S., Harrison, J. K., Opie, S. R., Zolotukhin, S., Muzyczka, N. (2004). Adeno-Associated Virus Type 2 VP2 Capsid Protein Is Nonessential and Can Tolerate Large Peptide Insertions at Its N Terminus. J. Virol. 78: 6595-6609 [Abstract] [Full Text]  
  • Yamamoto, N., Jiang, P., Yang, M., Xu, M., Yamauchi, K., Tsuchiya, H., Tomita, K., Wahl, G. M., Moossa, A. R., Hoffman, R. M. (2004). Cellular Dynamics Visualized in Live Cells in Vitro and in Vivo by Differential Dual-Color Nuclear-Cytoplasmic Fluorescent-Protein Expression. Cancer Res. 64: 4251-4256 [Abstract] [Full Text]  
  • Prima, V., Tennant, M., Gorbatyuk, O. S., Muzyczka, N., Scarpace, P. J., Zolotukhin, S. (2004). Differential Modulation of Energy Balance by Leptin, Ciliary Neurotrophic Factor, and Leukemia Inhibitory Factor Gene Delivery: Microarray Deoxyribonucleic Acid-Chip Analysis of Gene Expression. Endocrinology 145: 2035-2045 [Abstract] [Full Text]  
  • Tsitsigiannis, D. I., Zarnowski, R., Keller, N. P. (2004). The Lipid Body Protein, PpoA, Coordinates Sexual and Asexual Sporulation in Aspergillus nidulans. J. Biol. Chem. 279: 11344-11353 [Abstract] [Full Text]  
  • Shklyaev, S., Aslanidi, G., Tennant, M., Prima, V., Kohlbrenner, E., Kroutov, V., Campbell-Thompson, M., Crawford, J., Shek, E. W., Scarpace, P. J., Zolotukhin, S. (2003). Sustained peripheral expression of transgene adiponectin offsets the development of diet-induced obesity in rats. Proc. Natl. Acad. Sci. USA 100: 14217-14222 [Abstract] [Full Text]  
  • Heilbronn, R., Engstler, M., Weger, S., Krahn, A., Schetter, C., Boshart, M. (2003). ssDNA-dependent colocalization of adeno-associated virus Rep and herpes simplex virus ICP8 in nuclear replication domains. Nucleic Acids Res 31: 6206-6213 [Abstract] [Full Text]  
  • Kern, A., Schmidt, K., Leder, C., Muller, O. J., Wobus, C. E., Bettinger, K., Von der Lieth, C. W., King, J. A., Kleinschmidt, J. A. (2003). Identification of a Heparin-Binding Motif on Adeno-Associated Virus Type 2 Capsids. J. Virol. 77: 11072-11081 [Abstract] [Full Text]  
  • Zhang, F., Hackett, N. R., Lam, G., Cheng, J., Pergolizzi, R., Luo, L., Shmelkov, S. V., Edelberg, J., Crystal, R. G., Rafii, S. (2003). Green fluorescent protein selectively induces HSP70-mediated up-regulation of COX-2 expression in endothelial cells. Blood 102: 2115-2121 [Abstract] [Full Text]  
  • Young, J. E., Vogt, T., Gross, K. W., Khani, S. C. (2003). A Short, Highly Active Photoreceptor-Specific Enhancer/Promoter Region Upstream of the Human Rhodopsin Kinase Gene. IOVS 44: 4076-4085 [Abstract] [Full Text]  
  • Yang, X. (2003). Imaging of Vascular Gene Therapy. Radiology 228: 36-49 [Abstract] [Full Text]  
  • Opie, S. R., Warrington, K. H. Jr., Agbandje-McKenna, M., Zolotukhin, S., Muzyczka, N. (2003). Identification of Amino Acid Residues in the Capsid Proteins of Adeno-Associated Virus Type 2 That Contribute to Heparan Sulfate Proteoglycan Binding. J. Virol. 77: 6995-7006 [Abstract] [Full Text]  
  • Petrin, D., Baker, A., Coupland, S. G., Liston, P., Narang, M., Damji, K., Leonard, B., Chiodo, V. A., Timmers, A., Hauswirth, W., Korneluk, R. G., Tsilfidis, C. (2003). Structural and Functional Protection of Photoreceptors from MNU-Induced Retinal Degeneration by the X-Linked Inhibitor of Apoptosis. IOVS 44: 2757-2763 [Abstract] [Full Text]  
  • Smith, A. D., Collaco, R. F., Trempe, J. P. (2003). Enhancement of Recombinant Adeno-Associated Virus Type 2-Mediated Transgene Expression in a Lung Epithelial Cell Line by Inhibition of the Epidermal Growth Factor Receptor. J. Virol. 77: 6394-6404 [Abstract] [Full Text]  
  • Huser, D., Weger, S., Heilbronn, R. (2003). Packaging of Human Chromosome 19-Specific Adeno-Associated Virus (AAV) Integration Sites in AAV Virions during AAV Wild-Type and Recombinant AAV Vector Production. J. Virol. 77: 4881-4887 [Abstract] [Full Text]  
  • Chen, S., Agarwal, A., Glushakova, O. Y., Jorgensen, M. S., Salgar, S. K., Poirier, A., Flotte, T. R., Croker, B. P., Madsen, K. M., Atkinson, M. A., Hauswirth, W. W., Berns, K. I., Tisher, C. C. (2003). Gene Delivery in Renal Tubular Epithelial Cells Using Recombinant Adeno-Associated Viral Vectors. J. Am. Soc. Nephrol. 14: 947-958 [Abstract] [Full Text]  
  • Wrzesinski, C., Tesfay, L., Salome, N., Jauniaux, J.-C., Rommelaere, J., Cornelis, J., Dinsart, C. (2003). Chimeric and Pseudotyped Parvoviruses Minimize the Contamination of Recombinant Stocks with Replication-Competent Viruses and Identify a DNA Sequence That Restricts Parvovirus H-1 in Mouse Cells. J. Virol. 77: 3851-3858 [Abstract] [Full Text]  
  • Klocker, U., Oberwinkler, H., Kurschner, T., Protzer, U. (2003). Presence of Replicating Virus in Recombinant Hepadnavirus Stocks Results from Recombination and Can Be Eliminated by the Use of a Packaging Cell Line. J. Virol. 77: 2873-2881 [Abstract] [Full Text]  
  • Kreft, S. G., Nassal, M. (2003). hRUL138, a novel human RNA-binding RING-H2 ubiquitin-protein ligase. J. Cell Sci. 116: 605-616 [Abstract] [Full Text]  
  • Cannon, M., Philpott, N. J., Cesarman, E. (2002). The Kaposi's Sarcoma-Associated Herpesvirus G Protein-Coupled Receptor Has Broad Signaling Effects in Primary Effusion Lymphoma Cells. J. Virol. 77: 57-67 [Abstract] [Full Text]  
  • Philpott, N. J., Gomos, J., Berns, K. I., Falck-Pedersen, E. (2002). A p5 integration efficiency element mediates Rep-dependent integration into AAVS1 at chromosome 19. Proc. Natl. Acad. Sci. USA 99: 12381-12385 [Abstract] [Full Text]  
  • Seiler, M. P., Luner, P., Moninger, T. O., Karp, P. H., Keshavjee, S., Zabner, J. (2002). Thixotropic Solutions Enhance Viral-Mediated Gene Transfer to Airway Epithelia. Am. J. Respir. Cell Mol. Bio. 27: 133-140 [Abstract] [Full Text]  
  • Zaiss, A.-K., Son, S., Chang, L.-J. (2002). RNA 3' Readthrough of Oncoretrovirus and Lentivirus: Implications for Vector Safety and Efficacy. J. Virol. 76: 7209-7219 [Abstract] [Full Text]  
  • Mori, K., Gehlbach, P., Yamamoto, S., Duh, E., Zack, D. J., Li, Q., Berns, K. I., Raisler, B. J., Hauswirth, W. W., Campochiaro, P. A. (2002). AAV-Mediated Gene Transfer of Pigment Epithelium-Derived Factor Inhibits Choroidal Neovascularization. IOVS 43: 1994-2000 [Abstract] [Full Text]  
  • Alesci, S., Ramsey, W. J., Bornstein, S. R., Chrousos, G. P., Hornsby, P. J., Benvenga, S., Trimarchi, F., Ehrhart-Bornstein, M. (2002). Adenoviral vectors can impair adrenocortical steroidogenesis: Clinical implications for natural infections and gene therapy. Proc. Natl. Acad. Sci. USA 99: 7484-7489 [Abstract] [Full Text]  
  • Cheng, L., Sapieha, P., Kittlerova, P., Hauswirth, W. W., Di Polo, A. (2002). TrkB Gene Transfer Protects Retinal Ganglion Cells from Axotomy-Induced Death In Vivo. J. Neurosci. 22: 3977-3986 [Abstract] [Full Text]  
  • Philpott, N. J., Giraud-Wali, C., Dupuis, C., Gomos, J., Hamilton, H., Berns, K. I., Falck-Pedersen, E. (2002). Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5-rep Sequence in cis. J. Virol. 76: 5411-5421 [Abstract] [Full Text]  
  • Patnaik, A., Chau, V., Li, F., Montelaro, R. C., Wills, J. W. (2002). Budding of Equine Infectious Anemia Virus Is Insensitive to Proteasome Inhibitors. J. Virol. 76: 2641-2647 [Abstract] [Full Text]  
  • Li, L., Okura, M., Imamoto, A. (2002). Focal Adhesions Require Catalytic Activity of Src Family Kinases To Mediate Integrin-Matrix Adhesion. Mol. Cell. Biol. 22: 1203-1217 [Abstract] [Full Text]  
  • Casimiro, D. R., Tang, A., Perry, H. C., Long, R. S., Chen, M., Heidecker, G. J., Davies, M.-E., Freed, D. C., Persaud, N. V., Dubey, S., Smith, J. G., Havlir, D., Richman, D., Chastain, M. A., Simon, A. J., Fu, T.-M., Emini, E. A., Shiver, J. W. (2002). Vaccine-Induced Immune Responses in Rodents and Nonhuman Primates by Use of a Humanized Human Immunodeficiency Virus Type 1 pol Gene. J. Virol. 76: 185-194 [Abstract] [Full Text]  
  • AEsoy, R., Mellgren, G., Morohashi, K.-I., Lund, J. (2002). Activation of cAMP-Dependent Protein Kinase Increases the Protein Level of Steroidogenic Factor-1. Endocrinology 143: 295-303 [Abstract] [Full Text]  
  • Zentilin, L., Marcello, A., Giacca, M. (2001). Involvement of Cellular Double-Stranded DNA Break Binding Proteins in Processing of the Recombinant Adeno-Associated Virus Genome. J. Virol. 75: 12279-12287 [Abstract] [Full Text]  
  • Urrutia, A. O., Hurst, L. D. (2001). Codon Usage Bias Covaries With Expression Breadth and the Rate of Synonymous Evolution in Humans, but This Is Not Evidence for Selection. Genetics 159: 1191-1199 [Abstract] [Full Text]  
  • Leder, C., Kleinschmidt, J. A., Wiethe, C., Muller, M. (2001). Enhancement of Capsid Gene Expression: Preparing the Human Papillomavirus Type 16 Major Structural Gene L1 for DNA Vaccination Purposes. J. Virol. 75: 9201-9209 [Abstract] [Full Text]  
  • Phillips, M. I. (2001). Gene Therapy for Hypertension: The Preclinical Data. Hypertension 38: 543-548 [Abstract] [Full Text]  
  • Mack, V., Burnashev, N., Kaiser, K. M. M., Rozov, A., Jensen, V., Hvalby, O., Seeburg, P. H., Sakmann, B., Sprengel, R. (2001). Conditional Restoration of Hippocampal Synaptic Potentiation in GluR-A-Deficient Mice. Science 292: 2501-2504 [Abstract] [Full Text]  
  • Krestel, H. E., Mayford, M., Seeburg, P. H., Sprengel, R. (2001). A GFP-equipped bidirectional expression module well suited for monitoring tetracycline-regulated gene expression in mouse. Nucleic Acids Res 29: e39-e39 [Abstract] [Full Text]  
  • Yang, X., Liu, H., Li, D., Zhou, X., Jung, W. C., Deans, A. E., Cui, Y., Cheng, L. (2001). Digital Optical Imaging of Green Fluorescent Proteins for Tracking Vascular Gene Expression: Feasibility Study in Rabbit and Human Cell Models. Radiology 219: 171-175 [Abstract] [Full Text]  
  • Sandalon, Z., Gnatenko, D. V., Bahou, W. F., Hearing, P. (2000). Adeno-Associated Virus (AAV) Rep Protein Enhances the Generation of a Recombinant Mini-Adenovirus (Ad) Utilizing an Ad/AAV Hybrid Virus. J. Virol. 74: 10381-10389 [Abstract] [Full Text]  
  • JONES, C. A., HURLEY, M. I., BLACK, T. A., KANE, C. M., PAN, L., PRUITT, S. C., GROSS, K. W. (2000). Expression of a renin/GFP transgene in mouse embryonic, extra-embryonic, and adult tissues. Physiol. Genomics 4: 75-81 [Abstract] [Full Text]  
  • Chen, X., Stone, M., Schlagnhaufer, C., Romaine, C. P. (2000). A Fruiting Body Tissue Method for Efficient Agrobacterium-Mediated Transformation of Agaricus bisporus. Appl. Environ. Microbiol. 66: 4510-4513 [Abstract] [Full Text]  
  • Haberman, R. P., McCown, T. J., Samulski, R. J. (2000). Novel Transcriptional Regulatory Signals in the Adeno-Associated Virus Terminal Repeat A/D Junction Element. J. Virol. 74: 8732-8739 [Abstract] [Full Text]  
  • Molesworth, S. J., Lake, C. M., Borza, C. M., Turk, S. M., Hutt-Fletcher, L. M. (2000). Epstein-Barr Virus gH Is Essential for Penetration of B Cells but Also Plays a Role in Attachment of Virus to Epithelial Cells. J. Virol. 74: 6324-6332 [Abstract] [Full Text]  
  • Marschall, M., Freitag, M., Weiler, S., Sorg, G., Stamminger, T. (2000). Recombinant Green Fluorescent Protein-Expressing Human Cytomegalovirus as a Tool for Screening Antiviral Agents. Antimicrob. Agents Chemother. 44: 1588-1597 [Abstract] [Full Text]  
  • Kotsopoulou, E., Kim, V. N., Kingsman, A. J., Kingsman, S. M., Mitrophanous, K. A. (2000). A Rev-Independent Human Immunodeficiency Virus Type 1 (HIV-1)-Based Vector That Exploits a Codon-Optimized HIV-1 gag-pol Gene. J. Virol. 74: 4839-4852 [Abstract] [Full Text]  
  • Howe, D. G., McCarthy, K. D. (2000). Retroviral Inhibition of cAMP-Dependent Protein Kinase Inhibits Myelination But Not Schwann Cell Mitosis Stimulated by Interaction with Neurons. J. Neurosci. 20: 3513-3521 [Abstract] [Full Text]  
  • Malik, A. K., Monahan, P. E., Allen, D. L., Chen, B.-G., Samulski, R. J., Kurachi, K. (2000). Kinetics of Recombinant Adeno-Associated Virus-Mediated Gene Transfer. J. Virol. 74: 3555-3565 [Abstract] [Full Text]  
  • Schaley, J., O'Connor, R. J., Taylor, L. J., Bar-Sagi, D., Hearing, P. (2000). Induction of the Cellular E2F-1 Promoter by the Adenovirus E4-6/7 Protein. J. Virol. 74: 2084-2093 [Abstract] [Full Text]  
  • Moore, A., Marecos, E., Bogdanov, A. Jr, Weissleder, R. (2000). Tumoral Distribution of Long-circulating Dextran-coated Iron Oxide Nanoparticles in a Rodent Model. Radiology 214: 568-574 [Abstract] [Full Text]  
  • Suter, K. J., Song, W. J., Sampson, T. L., Wuarin, J.-P., Saunders, J. T., Dudek, F. E., Moenter, S. M. (2000). Genetic Targeting of Green Fluorescent Protein to Gonadotropin-Releasing Hormone Neurons: Characterization of Whole-Cell Electrophysiological Properties and Morphology. Endocrinology 141: 412-419 [Abstract] [Full Text]  
  • Ramalingam, R., Rafii, S., Worgall, S., Hackett, N. R., Crystal, R. G. (1999). Induction of Endogenous Genes following Infection of Human Endothelial Cells with an E1- E4+ Adenovirus Gene Transfer Vector. J. Virol. 73: 10183-10190 [Abstract] [Full Text]  
  • Corish, P., Tyler-Smith, C. (1999). Attenuation of green fluorescent protein half-life in mammalian cells. Protein Eng Des Sel 12: 1035-1040 [Abstract] [Full Text]  
  • Dubielzig, R., King, J. A., Weger, S., Kern, A., Kleinschmidt, J. A. (1999). Adeno-Associated Virus Type 2 Protein Interactions: Formation of Pre-Encapsidation Complexes. J. Virol. 73: 8989-8998 [Abstract] [Full Text]  
  • Gavin, D. K., Young, S. M. Jr., Xiao, W., Temple, B., Abernathy, C. R., Pereira, D. J., Muzyczka, N., Samulski, R. J. (1999). Charge-to-Alanine Mutagenesis of the Adeno-Associated Virus Type 2 Rep78/68 Proteins Yields Temperature-Sensitive and Magnesium-Dependent Variants. J. Virol. 73: 9433-9445 [Abstract] [Full Text]  
  • Beck, S. E., Jones, L. A., Chesnut, K., Walsh, S. M., Reynolds, T. C., Carter, B. J., Askin, F. B., Flotte, T. R., Guggino, W. B. (1999). Repeated Delivery of Adeno-Associated Virus Vectors to the Rabbit Airway. J. Virol. 73: 9446-9455 [Abstract] [Full Text]  
  • Guy, J., Qi, X., Wang, H., Hauswirth, W. W. (1999). Adenoviral Gene Therapy With Catalase Suppresses Experimental Optic Neuritis. Arch Ophthalmol 117: 1533-1539 [Abstract] [Full Text]  
  • Hernandez, Y. J., Wang, J., Kearns, W. G., Loiler, S., Poirier, A., Flotte, T. R. (1999). Latent Adeno-Associated Virus Infection Elicits Humoral but Not Cell-Mediated Immune Responses in a Nonhuman Primate Model. J. Virol. 73: 8549-8558 [Abstract] [Full Text]  
  • Protzer, U., Nassal, M., Chiang, P.-W., Kirschfink, M., Schaller, H. (1999). Interferon gene transfer by a hepatitis B virus vector efficiently suppresses wild-type virus infection. Proc. Natl. Acad. Sci. USA 96: 10818-10823 [Abstract] [Full Text]  
  • Sowa, G., Liu, J., Papapetropoulos, A., Rex-Haffner, M., Hughes, T. E., Sessa, W. C. (1999). Trafficking of Endothelial Nitric-oxide Synthase in Living Cells. QUANTITATIVE EVIDENCE SUPPORTING THE ROLE OF PALMITOYLATION AS A KINETIC TRAPPING MECHANISM LIMITING MEMBRANE DIFFUSION. J. Biol. Chem. 274: 22524-22531 [Abstract] [Full Text]  
  • Guy, J., Qi, X., Muzyczka, N., Hauswirth, W. W. (1999). Reporter Expression Persists 1 Year After Adeno-Associated Virus-Mediated Gene Transfer to the Optic Nerve. Arch Ophthalmol 117: 929-937 [Abstract] [Full Text]  
  • Zhou, J., Liu, W. J., Peng, S. W., Sun, X. Y., Frazer, I. (1999). Papillomavirus Capsid Protein Expression Level Depends on the Match between Codon Usage and tRNA Availability. J. Virol. 73: 4972-4982 [Abstract] [Full Text]  
  • Ramalingam, R., Rafii, S., Worgall, S., Brough, D. E., Crystal, R. G. (1999). E1-E4+ Adenoviral Gene Transfer Vectors Function as a "Pro-Life" Signal to Promote Survival of Primary Human Endothelial Cells. Blood 93: 2936-2944 [Abstract] [Full Text]  
  • Walters, R. W., Grunst, T., Bergelson, J. M., Finberg, R. W., Welsh, M. J., Zabner, J. (1999). Basolateral Localization of Fiber Receptors Limits Adenovirus Infection from the Apical Surface of Airway Epithelia. J. Biol. Chem. 274: 10219-10226 [Abstract] [Full Text]  
  • Holt, J. R., Johns, D. C., Wang, S., Chen, Z.-Y., Dunn, R. J., Marban, E., Corey, D. P. (1999). Functional Expression of Exogenous Proteins in Mammalian Sensory Hair Cells Infected With Adenoviral Vectors. J. Neurophysiol. 81: 1881-1888 [Abstract] [Full Text]  
  • Day, R. N., Nordeen, S. K., Wan, Y. (1999). Visualizing Protein-Protein Interactions in the Nucleus of the Living Cell. Mol. Endocrinol. 13: 517-526 [Full Text]  
  • Spergel, D. J., Kruth, U., Hanley, D. F., Sprengel, R., Seeburg, P. H. (1999). GABA- and Glutamate-Activated Channels in Green Fluorescent Protein-Tagged Gonadotropin-Releasing Hormone Neurons in Transgenic Mice. J. Neurosci. 19: 2037-2050 [Abstract] [Full Text]  
  • Kratz, P. A., Bottcher, B., Nassal, M. (1999). Native display of complete foreign protein domains on the surface of hepatitis B virus capsids. Proc. Natl. Acad. Sci. USA 96: 1915-1920 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»