This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ulaeto, D. Articles by Hruby, D. E. Search for Related Content PubMed PubMed Citation Articles by Ulaeto, D. Articles by Hruby, D. E.

 Previous Article  |  Next Article 

J. Virol., 06 1996, 3372-3377, Vol 70, No. 6
Copyright © 1996, American Society for Microbiology

The vaccinia virus 4c and A-type inclusion proteins are specific markers for the intracellular mature virus particle

D Ulaeto, D Grosenbach and DE Hruby
Chemical & Biological Defence Establishment, Porton Down, Salisbury, United Kingdom.

Gel analysis of vaccinia virus particles purified by buoyant [correction of bouyant] density demonstrates a protein with an estimated molecular mass of 59 kDa, which is apparently restricted to the intracellular mature virion (IMV) form. Western blotting (immunoblotting) and immunoprecipitation procedures identify the protein as the vaccinia virus 4c protein, which facilitates occlusion of poxvirus particles within cowpox cytoplasmic inclusions. Western blotting procedures also identify the truncated A-type inclusion protein of vaccinia virus as a specific marker for IMV particles. Kinetic analyses of virion maturation and 4c production suggest that peak enveloped virion production occurs before peak IMV production in the virus replication cycle and that 4c production is concomitant with maturation of IMV. The implications for a distinct and evolutionarily conserved function of IMV in viral pathogenesis are discussed.

This article has been cited by other articles:

• Domi, A., Weisberg, A. S., Moss, B. (2008). Vaccinia Virus E2L Null Mutants Exhibit a Major Reduction in Extracellular Virion Formation and Virus Spread. J. Virol. 82: 4215-4226 [Abstract] [Full Text]  
• Chiu, W.-L., Lin, C.-L., Yang, M.-H., Tzou, D.-L. M., Chang, W. (2007). Vaccinia Virus 4c (A26L) Protein on Intracellular Mature Virus Binds to the Extracellular Cellular Matrix Laminin. J. Virol. 81: 2149-2157 [Abstract] [Full Text]  
• Husain, M., Moss, B. (2005). Role of Receptor-Mediated Endocytosis in the Formation of Vaccinia Virus Extracellular Enveloped Particles. J. Virol. 79: 4080-4089 [Abstract] [Full Text]  
• Delhon, G., Tulman, E. R., Afonso, C. L., Lu, Z., de la Concha-Bermejillo, A., Lehmkuhl, H. D., Piccone, M. E., Kutish, G. F., Rock, D. L. (2004). Genomes of the Parapoxviruses Orf Virus and Bovine Papular Stomatitis Virus. J. Virol. 78: 168-177 [Abstract] [Full Text]  
• Smith, G. L., Vanderplasschen, A., Law, M. (2002). The formation and function of extracellular enveloped vaccinia virus. J. Gen. Virol. 83: 2915-2931 [Abstract] [Full Text]  
• McKelvey, T. A., Andrews, S. C., Miller, S. E., Ray, C. A., Pickup, D. J. (2002). Identification of the Orthopoxvirus p4c Gene, Which Encodes a Structural Protein That Directs Intracellular Mature Virus Particles into A-Type Inclusions. J. Virol. 76: 11216-11225 [Abstract] [Full Text]  
• Boulanger, D., Green, P., Jones, B., Henriquet, G., Hunt, L. G., Laidlaw, S. M., Monaghan, P., Skinner, M. A. (2002). Identification and Characterization of Three Immunodominant Structural Proteins of Fowlpox Virus. J. Virol. 76: 9844-9855 [Abstract] [Full Text]