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J. Virol., May 1996, 3242-3247, Vol 70, No. 5
Copyright © 1996, American Society for Microbiology

Subcellular localization of Aleutian mink disease parvovirus proteins and DNA during permissive infection of Crandell feline kidney cells

MB Oleksiewicz, F Costello, M Huhtanen, JB Wolfinbarger, S Alexandersen and ME Bloom
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Insitute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA.

Confocal microscopy allowed us to localize viral nonstructural (NS) and capsid (VP) proteins and DNA simultaneously in cells permissively infected with Aleutian mink disease parvovirus (ADV). Early after infection, NS proteins colocalized with viral DNA to form intranuclear inclusions, whereas VP proteins formed hollow intranuclear shells around the inclusions. Later, nuclei had irregular outlines and were virtually free of ADV products. In these cells, inclusions of viral DNA with or without associated NS protein were embedded in cytoplasmic VP protein. These findings implied that ADV replication within an infected cell is regulated spatially as well as temporally.

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