J. Virol., Apr 1996, 2369-2377, Vol 70, No. 4
Copyright © 1996, American Society for Microbiology

Transactivation of a cellular promoter by the NS1 protein of the parvovirus minute virus of mice through a putative hormone-responsive element

JM Vanacker, R Corbau, G Adelmant, M Perros, V Laudet and J Rommelaere
Tumor Virology Unit, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

The promoter of the thyroid hormone receptor alpha gene (c-erbA-1) is activated by the nonstructural protein 1 (NS1) of parvovirus minute virus of mice (prototype strain [MVMp]) in ras-transformed FREJ4 cells that are permissive for lytic MVMp replication. This stimulation may be related to the sensitivity of host cells to MVMp, as it does not take place in parental FR3T3 cells, which are resistant to the parvovirus killing effect. The analysis of a series of deletion and point mutants of the c-erbA-1 promoter led to the identification of an upstream region that is necessary for NS1-driven transactivation. This sequence harbors a putative hormone-responsive element and is sufficient to render a minimal promoter NS1 inducible in FREJ4 but not in FR3T3 cells, and it is involved in distinct interactions with proteins from the respective cell lines. The NS1-responsive element of the c-erbA-1 promoter bears no homology with sequences that were previously reported to be necessary for NS1 DNA binding and transactivation. Altogether, our data point to a novel, cell-specific mechanism of promoter activation by NS1.

This article has been cited by other articles:

• Li, J., Werner, E., Hergenhahn, M., Poirey, R., Luo, Z., Rommelaere, J., Jauniaux, J.-C. (2005). Expression Profiling of Human Hepatoma Cells Reveals Global Repression of Genes Involved in Cell Proliferation, Growth, and Apoptosis upon Infection with Parvovirus H-1. J. Virol. 79: 2274-2286 [Abstract] [Full Text]  
• Daeffler, L., Horlein, R., Rommelaere, J., Nuesch, J. P. F. (2003). Modulation of Minute Virus of Mice Cytotoxic Activities through Site-Directed Mutagenesis within the NS Coding Region. J. Virol. 77: 12466-12478 [Abstract] [Full Text]  
• Lachmann, S., Rommeleare, J., Nuesch, J. P. F. (2003). Novel PKC{eta} Is Required To Activate Replicative Functions of the Major Nonstructural Protein NS1 of Minute Virus of Mice. J. Virol. 77: 8048-8060 [Abstract] [Full Text]  
• Eichwald, V., Daeffler, L., Klein, M., Rommelaere, J., Salome, N. (2002). The NS2 Proteins of Parvovirus Minute Virus of Mice Are Required for Efficient Nuclear Egress of Progeny Virions in Mouse Cells. J. Virol. 76: 10307-10319 [Abstract] [Full Text]  
• Nuesch, J. P. F., Christensen, J., Rommelaere, J. (2001). Initiation of Minute Virus of Mice DNA Replication Is Regulated at the Level of Origin Unwinding by Atypical Protein Kinase C Phosphorylation of NS1. J. Virol. 75: 5730-5739 [Abstract] [Full Text]  
• Dettwiler, S., Rommelaere, J., Nüesch, J. P. F. (1999). DNA Unwinding Functions of Minute Virus of Mice NS1 Protein Are Modulated Specifically by the Lambda Isoform of Protein Kinase C. J. Virol. 73: 7410-7420 [Abstract] [Full Text]  
• Nuesch, J. P. F., Dettwiler, S., Corbau, R., Rommelaere, J. (1998). Replicative Functions of Minute Virus of Mice NS1 Protein Are Regulated In Vitro by Phosphorylation through Protein Kinase C. J. Virol. 72: 9966-9977 [Abstract] [Full Text]  
• Nuesch, J. P. F., Corbau, R., Tattersall, P., Rommelaere, J. (1998). Biochemical Activities of Minute Virus of Mice Nonstructural Protein NS1 Are Modulated In Vitro by the Phosphorylation State of the Polypeptide. J. Virol. 72: 8002-8012 [Abstract] [Full Text]  
• Deleu, L., Fuks, F., Spitkovsky, D., Hörlein, R., Faisst, S., Rommelaere, J. (1998). Opposite Transcriptional Effects of Cyclic AMP-Responsive Elements in Confluent or p27KIP-Overexpressing Cells versus Serum-Starved or Growing Cells. Mol. Cell. Biol. 18: 409-419 [Abstract] [Full Text]