The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Schnell, M. J.
	Articles by Rose, J. K.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Schnell, M. J.
	Articles by Rose, J. K.

J. Virol., Apr 1996, 2318-2323, Vol 70, No. 4
Copyright © 1996, American Society for Microbiology

The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus

MJ Schnell, L Buonocore, MA Whitt and JK Rose
Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA.

A new transcription unit was generated in the 3' noncoding region of the vesicular stomatitis virus (VSV) glycoprotein gene by introducing the smallest conserved sequence found at each VSV gene junction. This sequence was introduced into a DNA copy of the VSV genome from which infectious VSV can be derived. It contained an 11-nucleotide putative transcription stop/polyadenylation signal for the glycoprotein mRNA, an intergenic dinucleotide, and a 10-nucleotide putative transcription start sequence preceding a downstream foreign gene encoding the bacterial enzyme chloramphenicol acetyltransferase. Infectious recombinant VSV was recovered from this construct and was found to express high levels of functional chloramphenicol acetyltransferase mRNA and protein. The recombinant virus grew to wild-type titers of 5 x 10(9)/ml, and expression of the foreign gene was completely stable for at least 15 passages involving 10(6)-fold expansion at each passage. These results define functionally the transcription stop/polyadenylation and start sequences for VSV and also illustrate the utility of VSV as a stable vector that should have wide application in cell biology and vaccine development.

This article has been cited by other articles:

Rennick, L. J., Duprex, W. P., Rima, B. K. (2007). Measles virus minigenomes encoding two autofluorescent proteins reveal cell-to-cell variation in reporter expression dependent on viral sequences between the transcription units. J. Gen. Virol. 88: 2710-2718 [Abstract] [Full Text]
Ostertag, D., Hoblitzell-Ostertag, T. M., Perrault, J. (2007). Cell-Type-Specific Growth Restriction of Vesicular Stomatitis Virus polR Mutants Is Linked to Defective Viral Polymerase Function. J. Virol. 81: 492-502 [Abstract] [Full Text]
Ostertag, D., Hoblitzell-Ostertag, T. M., Perrault, J. (2007). Overproduction of Double-Stranded RNA in Vesicular Stomatitis Virus-Infected Cells Activates a Constitutive Cell-Type-Specific Antiviral Response. J. Virol. 81: 503-513 [Abstract] [Full Text]
Kohl, W., Grone, A., Moennig, V., Herrler, G. (2007). Expression of the surface glycoprotein E2 of Bovine viral diarrhea virus by recombinant vesicular stomatitis virus. J. Gen. Virol. 88: 157-165 [Abstract] [Full Text]
Bukreyev, A., Skiadopoulos, M. H., Murphy, B. R., Collins, P. L. (2006). Nonsegmented negative-strand viruses as vaccine vectors.. J. Virol. 80: 10293-10306 [Full Text]
Gao, Y., Whitaker-Dowling, P., Watkins, S. C., Griffin, J. A., Bergman, I. (2006). Rapid adaptation of a recombinant vesicular stomatitis virus to a targeted cell line.. J. Virol. 80: 8603-8612 [Abstract] [Full Text]
Cose, S., Brammer, C., Zammit, D. J., Blair, D., Lefrancois, L. (2006). CD4 T cells inhibit the CD8 T cell response during low-dose virus infection. Int Immunol 18: 1285-1293 [Abstract] [Full Text]
Publicover, J., Ramsburg, E., Robek, M., Rose, J. K. (2006). Rapid Pathogenesis Induced by a Vesicular Stomatitis Virus Matrix Protein Mutant: Viral Pathogenesis Is Linked to Induction of Tumor Necrosis Factor Alpha. J. Virol. 80: 7028-7036 [Abstract] [Full Text]
Natuk, R. J., Cooper, D., Guo, M., Calderon, P., Wright, K. J., Nasar, F., Witko, S., Pawlyk, D., Lee, M., DeStefano, J., Tummolo, D., Abramovitz, A. S., Gangolli, S., Kalyan, N., Clarke, D. K., Hendry, R. M., Eldridge, J. H., Udem, S. A., Kowalski, J. (2006). Recombinant Vesicular Stomatitis Virus Vectors Expressing Herpes Simplex Virus Type 2 gD Elicit Robust CD4+ Th1 Immune Responses and Are Protective in Mouse and Guinea Pig Models of Vaginal Challenge. J. Virol. 80: 4447-4457 [Abstract] [Full Text]
Ramsburg, E., Publicover, J., Buonocore, L., Poholek, A., Robek, M., Palin, A., Rose, J. K. (2005). A Vesicular Stomatitis Virus Recombinant Expressing Granulocyte-Macrophage Colony-Stimulating Factor Induces Enhanced T-Cell Responses and Is Highly Attenuated for Replication in Animals. J. Virol. 79: 15043-15053 [Abstract] [Full Text]
Publicover, J., Ramsburg, E., Rose, J. K. (2005). A Single-Cycle Vaccine Vector Based on Vesicular Stomatitis Virus Can Induce Immune Responses Comparable to Those Generated by a Replication-Competent Vector. J. Virol. 79: 13231-13238 [Abstract] [Full Text]
Hanika, A., Larisch, B., Steinmann, E., Schwegmann-Wessels, C., Herrler, G., Zimmer, G. (2005). Use of influenza C virus glycoprotein HEF for generation of vesicular stomatitis virus pseudotypes. J. Gen. Virol. 86: 1455-1465 [Abstract] [Full Text]
Leung, J., Esper, F., Weibel, C., Kahn, J. S. (2005). Seroepidemiology of Human Metapneumovirus (hMPV) on the Basis of a Novel Enzyme-Linked Immunosorbent Assay Utilizing hMPV Fusion Protein Expressed in Recombinant Vesicular Stomatitis Virus. J. Clin. Microbiol. 43: 1213-1219 [Abstract] [Full Text]
Publicover, J., Ramsburg, E., Rose, J. K. (2004). Characterization of Nonpathogenic, Live, Viral Vaccine Vectors Inducing Potent Cellular Immune Responses. J. Virol. 78: 9317-9324 [Abstract] [Full Text]
Schlehuber, L. D., Rose, J. K. (2004). Prediction and Identification of a Permissive Epitope Insertion Site in the Vesicular Stomatitis Virus Glycoprotein. J. Virol. 78: 5079-5087 [Abstract] [Full Text]
Garbutt, M., Liebscher, R., Wahl-Jensen, V., Jones, S., Moller, P., Wagner, R., Volchkov, V., Klenk, H.-D., Feldmann, H., Stroher, U. (2004). Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses. J. Virol. 78: 5458-5465 [Abstract] [Full Text]
Ramsburg, E., Rose, N. F., Marx, P. A., Mefford, M., Nixon, D. F., Moretto, W. J., Montefiori, D., Earl, P., Moss, B., Rose, J. K. (2004). Highly Effective Control of an AIDS Virus Challenge in Macaques by Using Vesicular Stomatitis Virus and Modified Vaccinia Virus Ankara Vaccine Vectors in a Single-Boost Protocol. J. Virol. 78: 3930-3940 [Abstract] [Full Text]
Roberts, A., Reuter, J. D., Wilson, J. H., Baldwin, S., Rose, J. K. (2004). Complete Protection from Papillomavirus Challenge after a Single Vaccination with a Vesicular Stomatitis Virus Vector Expressing High Levels of L1 Protein. J. Virol. 78: 3196-3199 [Abstract] [Full Text]
Porosnicu, M., Mian, A., Barber, G. N. (2003). The Oncolytic Effect of Recombinant Vesicular Stomatitis Virus Is Enhanced by Expression of the Fusion Cytosine Deaminase/Uracil Phosphoribosyltransferase Suicide Gene. Cancer Res. 63: 8366-8376 [Abstract] [Full Text]
Ebert, O., Shinozaki, K., Huang, T.-G., Savontaus, M. J., Garcia-Sastre, A., Woo, S. L. C. (2003). Oncolytic Vesicular Stomatitis Virus for Treatment of Orthotopic Hepatocellular Carcinoma in Immune-competent Rats. Cancer Res. 63: 3605-3611 [Abstract] [Full Text]
Neumann, G., Whitt, M. A., Kawaoka, Y. (2002). A decade after the generation of a negative-sense RNA virus from cloned cDNA - what have we learned?. J. Gen. Virol. 83: 2635-2662 [Abstract] [Full Text]
Ezelle, H. J., Markovic, D., Barber, G. N. (2002). Generation of Hepatitis C Virus-Like Particles by Use of a Recombinant Vesicular Stomatitis Virus Vector. J. Virol. 76: 12325-12334 [Abstract] [Full Text]
Whelan, S. P. J., Wertz, G. W. (2002). Transcription and replication initiate at separate sites on the vesicular stomatitis virus genome. Proc. Natl. Acad. Sci. USA 99: 9178-9183 [Abstract] [Full Text]
Haglund, K., Leiner, I., Kerksiek, K., Buonocore, L., Pamer, E., Rose, J. K. (2002). Robust Recall and Long-Term Memory T-Cell Responses Induced by Prime-Boost Regimens with Heterologous Live Viral Vectors Expressing Human Immunodeficiency Virus Type 1 Gag and Env Proteins. J. Virol. 76: 7506-7517 [Abstract] [Full Text]
Hinzman, E. E., Barr, J. N., Wertz, G. W. (2002). Identification of an Upstream Sequence Element Required for Vesicular Stomatitis Virus mRNA Transcription. J. Virol. 76: 7632-7641 [Abstract] [Full Text]
Wertz, G. W., Moudy, R., Ball, L. A. (2002). Adding Genes to the RNA Genome of Vesicular Stomatitis Virus: Positional Effects on Stability of Expression. J. Virol. 76: 7642-7650 [Abstract] [Full Text]
Buonocore, L., Blight, K. J., Rice, C. M., Rose, J. K. (2002). Characterization of Vesicular Stomatitis Virus Recombinants That Express and Incorporate High Levels of Hepatitis C Virus Glycoproteins. J. Virol. 76: 6865-6872 [Abstract] [Full Text]
Quinones-Kochs, M. I., Buonocore, L., Rose, J. K. (2002). Role of N-Linked Glycans in a Human Immunodeficiency Virus Envelope Glycoprotein: Effects on Protein Function and the Neutralizing Antibody Response. J. Virol. 76: 4199-4211 [Abstract] [Full Text]
Zimmer, G., Zimmer, K.-P., Trotz, I., Herrler, G. (2002). Vesicular Stomatitis Virus Glycoprotein Does Not Determine the Site of Virus Release in Polarized Epithelial Cells. J. Virol. 76: 4103-4107 [Abstract] [Full Text]
Haglund, K., Leiner, I., Kerksiek, K., Buonocore, L., Pamer, E., Rose, J. K. (2002). High-Level Primary CD8+ T-Cell Response to Human Immunodeficiency Virus Type 1 Gag and Env Generated by Vaccination with Recombinant Vesicular Stomatitis Viruses. J. Virol. 76: 2730-2738 [Abstract] [Full Text]
Schmidt, A. C., Wenzke, D. R., McAuliffe, J. M., St. Claire, M., Elkins, W. R., Murphy, B. R., Collins, P. L. (2002). Mucosal Immunization of Rhesus Monkeys against Respiratory Syncytial Virus Subgroups A and B and Human Parainfluenza Virus Type 3 by Using a Live cDNA-Derived Vaccine Based on a Host Range-Attenuated Bovine Parainfluenza Virus Type 3 Vector Backbone. J. Virol. 76: 1089-1099 [Abstract] [Full Text]
Barr, J. N., Wertz, G. W. (2001). Polymerase Slippage at Vesicular Stomatitis Virus Gene Junctions To Generate Poly(A) Is Regulated by the Upstream 3'-AUAC-5' Tetranucleotide: Implications for the Mechanism of Transcription Termination. J. Virol. 75: 6901-6913 [Abstract] [Full Text]
Huang, Z., Krishnamurthy, S., Panda, A., Samal, S. K. (2001). High-level expression of a foreign gene from the most 3'-proximal locus of a recombinant Newcastle disease virus. J. Gen. Virol. 82: 1729-1736 [Abstract] [Full Text]
Foley, H. D., McGettigan, J. P., Siler, C. A., Dietzschold, B., Schnell, M. J. (2000). A recombinant rabies virus expressing vesicular stomatitis virus glycoprotein fails to protect against rabies virus infection. Proc. Natl. Acad. Sci. USA 10.1073/pnas.011510698v1 [Abstract] [Full Text]
Rose, N. F., Roberts, A., Buonocore, L., Rose, J. K. (2000). Glycoprotein Exchange Vectors Based on Vesicular Stomatitis Virus Allow Effective Boosting and Generation of Neutralizing Antibodies to a Primary Isolate of Human Immunodeficiency Virus Type 1. J. Virol. 74: 10903-10910 [Abstract] [Full Text]
Bukreyev, A., Murphy, B. R., Collins, P. L. (2000). Respiratory Syncytial Virus Can Tolerate an Intergenic Sequence of at Least 160 Nucleotides with Little Effect on Transcription or Replication In Vitro and In Vivo. J. Virol. 74: 11017-11026 [Abstract] [Full Text]
Whelan, S. P. J., Barr, J. N., Wertz, G. W. (2000). Identification of a Minimal Size Requirement for Termination of Vesicular Stomatitis Virus mRNA: Implications for the Mechanism of Transcription. J. Virol. 74: 8268-8276 [Abstract] [Full Text]
Finke, S., Cox, J. H., Conzelmann, K.-K. (2000). Differential Transcription Attenuation of Rabies Virus Genes by Intergenic Regions: Generation of Recombinant Viruses Overexpressing the Polymerase Gene. J. Virol. 74: 7261-7269 [Abstract] [Full Text]
Bukreyev, A., Whitehead, S. S., Prussin, C., Murphy, B. R., Collins, P. L. (2000). Effect of Coexpression of Interleukin-2 by Recombinant Respiratory Syncytial Virus on Virus Replication, Immunogenicity, and Production of Other Cytokines. J. Virol. 74: 7151-7157 [Abstract] [Full Text]
Duprex, W. P., McQuaid, S., Hangartner, L., Billeter, M. A., Rima, B. K. (1999). Observation of Measles Virus Cell-to-Cell Spread in Astrocytoma Cells by Using a Green Fluorescent Protein-Expressing Recombinant Virus. J. Virol. 73: 9568-9575 [Abstract] [Full Text]
Stillman, E. A., Whitt, M. A. (1999). Transcript Initiation and 5'-End Modifications Are Separable Events during Vesicular Stomatitis Virus Transcription. J. Virol. 73: 7199-7209 [Abstract] [Full Text]
Boritz, E., Gerlach, J., Johnson, J. E., Rose, J. K. (1999). Replication-Competent Rhabdoviruses with Human Immunodeficiency Virus Type 1 Coats and Green Fluorescent Protein: Entry by a pH-Independent Pathway. J. Virol. 73: 6937-6945 [Abstract] [Full Text]
Baron, M. D., Foster-Cuevas, M., Baron, J., Barrett, T. (1999). Expression in cattle of epitopes of a heterologous virus using a recombinant rinderpest virus. J. Gen. Virol. 80: 2031-2039 [Abstract] [Full Text]
Ball, L. A., Pringle, C. R., Flanagan, B., Perepelitsa, V. P., Wertz, G. W. (1999). Phenotypic Consequences of Rearranging the P, M, and G Genes of Vesicular Stomatitis Virus. J. Virol. 73: 4705-4712 [Abstract] [Full Text]
Lefrancois, L., Parker, C. M., Olson, S., Muller, W., Wagner, N., Puddington, L. (1999). The Role of beta 7 Integrins in CD8 T Cell Trafficking During an Antiviral Immune Response. J. Exp. Med. 189: 1631-1638 [Abstract] [Full Text]
Roberts, A., Buonocore, L., Price, R., Forman, J., Rose, J. K. (1999). Attenuated Vesicular Stomatitis Viruses as Vaccine Vectors. J. Virol. 73: 3723-3732 [Abstract] [Full Text]
Whelan, S. P.�J., Wertz, G. W. (1999). Regulation of RNA Synthesis by the Genomic Termini of Vesicular Stomatitis Virus: Identification of Distinct Sequences Essential for Transcription but Not Replication. J. Virol. 73: 297-306 [Abstract] [Full Text]
Kim, S.-K., Reed, D. S., Olson, S., Schnell, M. J., Rose, J. K., Morton, P. A., Lefrancois, L. (1998). Generation of mucosal cytotoxic T cells against soluble protein by tissue-specific environmental and costimulatory signals. Proc. Natl. Acad. Sci. USA 95: 10814-10819 [Abstract] [Full Text]
Stillman, E. A., Whitt, M. A. (1998). The Length and Sequence Composition of Vesicular Stomatitis Virus Intergenic Regions Affect mRNA Levels and the Site of Transcript Initiation. J. Virol. 72: 5565-5572 [Abstract] [Full Text]
Moser, C., Tratschin, J.-D., Hofmann, M. A. (1998). A Recombinant Classical Swine Fever Virus Stably Expresses a Marker Gene. J. Virol. 72: 5318-5322 [Abstract] [Full Text]
Drake, J. W., Charlesworth, B., Charlesworth, D., Crow, J. F. (1998). Rates of Spontaneous Mutation. Genetics 148: 1667-1686 [Abstract] [Full Text]
Hwang, L. N., Englund, N., Pattnaik, A. K. (1998). Polyadenylation of Vesicular Stomatitis Virus mRNA Dictates Efficient Transcription Termination at the Intercistronic Gene Junctions. J. Virol. 72: 1805-1813 [Abstract] [Full Text]
Takada, A., Robison, C., Goto, H., Sanchez, A., Murti, K. G., Whitt, M. A., Kawaoka, Y. (1997). A system for functional analysis of Ebola�virus�glycoprotein. Proc. Natl. Acad. Sci. USA 94: 14764-14769 [Abstract] [Full Text]
Rose, J.�K. (1996). Positive strands to the rescue again: A segmented negative-strand RNA virus derived from�cloned�cDNAs. Proc. Natl. Acad. Sci. USA 93: 14998-15000 [Full Text]
Foley, H. D., McGettigan, J. P., Siler, C. A., Dietzschold, B., Schnell, M. J. (2000). A recombinant rabies virus expressing vesicular stomatitis virus glycoprotein fails to protect against rabies virus infection. Proc. Natl. Acad. Sci. USA 97: 14680-14685 [Abstract] [Full Text]

J. Bacteriol.	Mol. Cell. Biol.	Microbiol. Mol. Biol. Rev.

Clin. Vaccine Immunol.	ALL ASM JOURNALS