J. Virol., 03 1996, 1433-1438, Vol 70, No. 3
Copyright © 1996, American Society for Microbiology

Characterization of two novel YY1 binding sites in the polyomavirus late promoter

F Martelli, C Iacobini, M Caruso and A Felsani
Istituto Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Rome, Italy.

NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, delta, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site- directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.

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