This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Purcell, D. F. Articles by Martin, M. A. Search for Related Content PubMed PubMed Citation Articles by Purcell, D. F. Articles by Martin, M. A.

 Previous Article  |  Next Article 

J. Virol., 02 1996, 887-897, Vol 70, No. 2
Copyright © 1996, American Society for Microbiology

An array of murine leukemia virus-related elements is transmitted and expressed in a primate recipient of retroviral gene transfer

DF Purcell, CM Broscius, EF Vanin, CE Buckler, AW Nienhuis and MA Martin
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Direct RNA-PCR analyses of T-cell lymphomas that developed in rhesus macaques during a gene transfer experiment revealed the presence of several different recombinant murine leukemia viruses (MuLV). Most prominent was the expected MuLV recombinant, designated MoLTRAmphoenv in which the amphotropic env of the helper packaging virus was joined to the long terminal repeat (LTR) of the Moloney MuLV-derived vector. This retrovirus does not exist in nature. An additional copy of the core enhancer acquired from the vector LTR may have augmented the replicative properties of MoLTRAmphoenv MuLV in several different rhesus cell types compared with the prototype amphotropic MuLV4070A. Unexpectedly, at least two types of mink cell focus-forming MuLV elements, arising from endogenous retroviral sequences expressed in the murine packaging cell line, were also transmitted and highly expressed in one of the macaques. Furthermore, murine virus-like VL-30 sequences were detected in the rhesus lymphomas, but these were not transcribed into RNA. The unanticipated presence of an array of MuLV-related structures in a primate gene transfer recipient demands ever-vigilant scrutiny for the existence of transmissible retroviral elements and replication-competent viruses possessing altered tropic or growth properties in packaging cells producing retroviral vectors.

This article has been cited by other articles:

• Steidl, S., Schule, S., Muhlebach, M. D., Stitz, J., Boller, K., Cichutek, K., Schweizer, M. (2004). Genetic engineering of onco/lentivirus hybrids results in formation of infectious but not of replication-competent viruses. J. Gen. Virol. 85: 665-678 [Abstract] [Full Text]  
• Hu, W.-S., Pathak, V. K. (2000). Design of Retroviral Vectors and Helper Cells for Gene Therapy. Pharmacol. Rev. 52: 493-512 [Abstract] [Full Text]  
• Palmarini, M., Hallwirth, C., York, D., Murgia, C., de Oliveira, T., Spencer, T., Fan, H. (2000). Molecular Cloning and Functional Analysis of Three Type D Endogenous Retroviruses of Sheep Reveal a Different Cell Tropism from That of the Highly Related Exogenous Jaagsiekte Sheep Retrovirus. J. Virol. 74: 8065-8076 [Abstract] [Full Text]  
• Kung, S. K. P., An, D. S., Chen, I. S. Y. (2000). A Murine Leukemia Virus (MuLV) Long Terminal Repeat Derived from Rhesus Macaques in the Context of a Lentivirus Vector and MuLV gag Sequence Results in High-Level Gene Expression in Human T Lymphocytes. J. Virol. 74: 3668-3681 [Abstract] [Full Text]  
• Mikkelsen, J. G., Lund, A. H., Duch, M., Pedersen, F. S. (1999). Forced recombination of {Psi}-modified murine leukaemia virus-based vectors with murine leukaemia-like and VL30 murine endogenous retroviruses. J. Gen. Virol. 80: 2957-2967 [Abstract] [Full Text]  
• Ristevski, S., Purcell, D. F. J., Marshall, J., Campagna, D., Nouri, S., Fenton, S. P., McPhee, D. A., Kannourakis, G. (1999). Novel Endogenous Type D Retroviral Particles Expressed at High Levels in a SCID Mouse Thymic Lymphoma. J. Virol. 73: 4662-4669 [Abstract] [Full Text]  
• White, S. M., Renda, M., Nam, N.-Y., Klimatcheva, E., Zhu, Y., Fisk, J., Halterman, M., Rimel, B. J., Federoff, H., Pandya, S., Rosenblatt, J. D., Planelles, V. (1999). Lentivirus Vectors Using Human and Simian Immunodeficiency Virus Elements. J. Virol. 73: 2832-2840 [Abstract] [Full Text]  
• Certo, J. L., Shook, B. F., Yin, P. D., Snider, J. T., Hu, W.-S. (1998). Nonreciprocal Pseudotyping: Murine Leukemia Virus Proteins Cannot Efficiently Package Spleen Necrosis Virus-Based Vector RNA. J. Virol. 72: 5408-5413 [Abstract] [Full Text]  
• Hoatlin, M. E., Gomez-Lucia, E., Lilly, F., Beckstead, J. H., Kabat, D. (1998). Origin and Rapid Evolution of a Novel Murine Erythroleukemia Virus of the Spleen Focus-Forming Virus Family. J. Virol. 72: 3602-3609 [Abstract] [Full Text]  
• Gomez-Lucia, E., Zhi, Y., Nabavi, M., Zhang, W., Kabat, D., Hoatlin, M. E. (1998). An Array of Novel Murine Spleen Focus-Forming Viruses That Activate the Erythropoietin Receptor. J. Virol. 72: 3742-3750 [Abstract] [Full Text]  
• Chong, H., Starkey, W., Vile, R. G. (1998). A Replication-Competent Retrovirus Arising from a Split-Function Packaging Cell Line Was Generated by Recombination Events between the Vector, One of the Packaging Constructs, and Endogenous Retroviral Sequences. J. Virol. 72: 2663-2670 [Abstract] [Full Text]  
• Patience, C., Takeuchi, Y., Cosset, F.-L., Weiss, R. A. (1998). Packaging of Endogenous Retroviral Sequences in Retroviral Vectors Produced by Murine and Human Packaging Cells. J. Virol. 72: 2671-2676 [Abstract] [Full Text]  
• Wilson, C. A., Wong, S., Muller, J., Davidson, C. E., Rose, T. M., Burd, P. (1998). Type C Retrovirus Released from Porcine Primary Peripheral Blood Mononuclear Cells Infects Human Cells. J. Virol. 72: 3082-3087 [Abstract] [Full Text]  
• Mikkelsen, J. G., Lund, A. H., Dybkar, K., Duch, M., Pedersen, F. S. (1998). Extended Minus-Strand DNA as Template for R-U5-Mediated Second-Strand Transfer in Recombinational Rescue of Primer Binding Site-Modified Retroviral Vectors. J. Virol. 72: 2519-2525 [Abstract] [Full Text]  
• Anderson, J. A., Bowman, E. H., Hu, W.-S. (1998). Retroviral Recombination Rates Do Not Increase Linearly with Marker Distance and Are Limited by the Size of the Recombining Subpopulation. J. Virol. 72: 1195-1202 [Abstract] [Full Text]  
• Strayer, D. S., Strayer, D. S. (1996). SV40 As an Effective Gene Transfer Vector in Vivo. J. Biol. Chem. 271: 24741-24746 [Abstract] [Full Text]