This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ray, S. Articles by Tegtmeyer, P. Search for Related Content PubMed PubMed Citation Articles by Ray, S. Articles by Tegtmeyer, P.

 Previous Article  |  Next Article 

J. Virol., 10 1996, 7224-7227, Vol 70, No. 10
Copyright © 1996, American Society for Microbiology

Differential interaction of temperature-sensitive simian virus 40 T antigens with tumor suppressors pRb and p53

S Ray, ME Anderson and P Tegtmeyer
Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, New York 11794-5222, USA.

Previous studies have shown that simian virus 40 large T antigen transforms cells by binding and inactivating suppressors of cell cycle progression and tumor formation. Here, we characterize the interactions of five temperature-sensitive T antigens with the tumor suppressor proteins pRb and p53. All five mutant T antigens bind pRb at the nonpermissive temperature with efficiencies similar to that of wild- type T antigen. A single transformation-competent mutant, with a substitution of amino acid 186, binds p53 at the nonpermissive temperature. Four transformation-defective mutants, with a substitution at T antigen position 357, 422, 427, or 438, are temperature sensitive for the binding and inactivation of p53. Our findings provide a basis for understanding the behavior of cells transformed by temperature- sensitive T antigens.