This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hubbs, A. E. Articles by Wright, C. F. Search for Related Content PubMed PubMed Citation Articles by Hubbs, A. E. Articles by Wright, C. F.

 Previous Article  |  Next Article 

J. Virol., 01 1996, 327-331, Vol 70, No. 1
Copyright © 1996, American Society for Microbiology

The A2L intermediate gene product is required for in vitro transcription from a vaccinia virus late promoter

AE Hubbs and CF Wright
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, D.C. 20306-6000, USA.

Previously, the in vitro late transcription system of vaccinia virus was resolved into four components: the 17- and 30-kDa products of the A1L and G8R intermediate genes, respectively, the viral DNA-dependent RNA polymerase, and an unmapped factor sedimenting at 32 to 38 kDa. Another protein, the 26-kDa product of the A2L open reading frame was predicted to be a late transcription factor on the basis of a transient- expression assay but was not recognized as being necessary for transcriptional activity in vitro. We now report that both the unmapped factor and the 26-kDa protein are required for transcription from a vaccinia virus late promoter in vitro. Since the 26-kDa protein has now been shown to be a trans-activator of late transcription and it is the product of a known gene, we suggest that it be designated VLTF-3.

This article has been cited by other articles:

• Katsafanas, G. C., Moss, B. (2004). Vaccinia Virus Intermediate Stage Transcription Is Complemented by Ras-GTPase-activating Protein SH3 Domain-binding Protein (G3BP) and Cytoplasmic Activation/Proliferation-associated Protein (p137) Individually or as a Heterodimer. J. Biol. Chem. 279: 52210-52217 [Abstract] [Full Text]  
• Shors, T., Keck, J. G., Moss, B. (1999). Down Regulation of Gene Expression by the Vaccinia Virus D10 Protein. J. Virol. 73: 791-796 [Abstract] [Full Text]  
• Gunasinghe, S. K., Hubbs, A. E., Wright, C. F. (1998). A Vaccinia Virus Late Transcription Factor with Biochemical and Molecular Identity to a Human Cellular Protein. J. Biol. Chem. 273: 27524-27530 [Abstract] [Full Text]  
• Nakai, H., Herzog, R. W., Hagstrom, J. N., Walter, J., Kung, S.-H., Yang, E. Y., Tai, S. J., Iwaki, Y., Kurtzman, G. J., Fisher, K. J., Colosi, P., Couto, L. B., High, K. A. (1998). Adeno-Associated Viral Vector-Mediated Gene Transfer of Human Blood Coagulation Factor IX Into Mouse Liver. Blood 91: 4600-4607 [Abstract] [Full Text]  
• Zhu, M., Moore, T., Broyles, S. S. (1998). A Cellular Protein Binds Vaccinia Virus Late Promoters and Activates Transcription In Vitro. J. Virol. 72: 3893-3899 [Abstract] [Full Text]  
• Wright, C. F., Hubbs, A. E., Gunasinghe, S. K., Oswald, B. W. (1998). A Vaccinia Virus Late Transcription Factor Copurifies with a Factor That Binds to a Viral Late Promoter and Is Complemented by Extracts from Uninfected HeLa Cells. J. Virol. 72: 1446-1451 [Abstract] [Full Text]  
• Wright, C. F., Oswald, B. W., Dellis, S. (2001). Vaccinia Virus Late Transcription Is Activated in Vitro by Cellular Heterogeneous Nuclear Ribonucleoproteins. J. Biol. Chem. 276: 40680-40686 [Abstract] [Full Text]