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J. Virol., 01 1996, 159-164, Vol 70, No. 1
E Kondo and HG Gottlinger
The vpr gene product of human immunodeficiency virus type (HIV-1) is a
virion-associated regulatory protein. A transferable virion association
motif for Vpr is located in the p6 domain of the HIV-1 Gag polyprotein. To
map the sequences in p6 that are involved in Vpr incorporation, we analyzed
the ability of mutant forms of p6 to direct the incorporation of Vpr into
chimeric viral particles. Our results show that the determinants which
govern Vpr incorporation are largely confined to a C- terminal region of
the p6 domain. Within this region, three hydrophobic residues in a highly
conserved sequence motif (L-X-S-L-F-G) are absolutely required. Remarkably,
the transfer of the conserved motif and of a single flanking residue to a
heterologous Gag polyprotein was sufficient to transfer the ability to
incorporate Vpr at moderate levels. The transfer of residues 32 to 46 of p6
led to Vpr incorporation levels that were comparable to those obtained with
full- length HIV-1 Gag protein, indicating that this region contains
essentially all the information required for efficient Vpr incorporation.
Copyright © 1996, American Society for Microbiology
A conserved LXXLF sequence is the major determinant in p6gag required for the incorporation of human immunodeficiency virus type 1 Vpr
Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
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