This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Aaslestad, H. G. Articles by Koprowski, H. Search for Related Content PubMed PubMed Citation Articles by Aaslestad, H. G. Articles by Koprowski, H.

 Previous Article  |  Next Article 

J Virol. 1971 June; 7(6): 726-735
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Comparison of the Ribonucleic Acid Polymerases of Two Rhabdoviruses, Kern Canyon Virus and Vesicular Stomatitis Virus

The Wistar Institute of Anatomy and Biology, The World Health Organization International Reference Center for Rabies at The Wistar Institute, Philadelphia, Pennsylvania 19104
The Institute of Cancer Research, Columbia University, New York, New York 10032

ABSTRACT

A ribonucleic acid (RNA)-dependent RNA polymerase has been demonstrated in Kern Canyon virus (KCV) particles. The RNA product of the KCV polymerase hybridizes to KCV viral RNA. The properties of this viral enzyme have been characterized and compared with those of vesicular stomatitis virus (VSV). RNA polymerases from both viruses require similar conditions of temperature, pH, and detergent and magnesium concentrations for maximal synthesis of RNA. The RNA polymerase contained in the virion of KCV was more dependent on the presence of a sulfhydryl agent than was the VSV enzyme. Under optimal conditions, the specific activity of the VSV polymerase is about twenty-five times as great as that of KCV.