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Three Size-Classes of Intracellular Adenovirus Deoxyribonucleic Acid ¹

The Rockefeller University, New York, New York 10021

ABSTRACT

When human adenovirus type 2 or 12 infects cells, either productively or non-productively, three classes of viral deoxyribonucleic acid (DNA) are found within the cells: (i) viral DNA which cosediments with DNA extracted from infectious adenovirions at 31.3S for adenovirus type 2 and at 29.0S for adenovirus type 12, (ii) viral DNA which sediments at about 18S, and (iii) viral DNA which sediments at >45S and is apparently integrated into the cellular DNA. A precursor-product relationship is suggested as a working hypothesis; the intact viral DNA is hydrolyzed to slowly sedimenting DNA and the slowly sedimenting DNA is integrated into the cellular DNA. Both the parental and the newly synthesized viral DNA are altered by this route. The intact viral DNA within the cells apparently is cleaved into the slowly sedimenting DNA by a preformed enzyme.

² Present address: Department of Cell Biology and Pharmacology, School of Medicine, University of Maryland, Baltimore, Md. 21201.

¹ Part of this report was taken from a thesis submitted by B. T. B. in partial fulfillment of the requirement for the Doctor of Philosophy degree from The Rockefeller University, New York, N.Y. A preliminary report was presented at the 1969 Annual Meeting of the Federation of American Societies for Experimental Biology.