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Comparison of Rous Sarcoma Virus-Specific Deoxyribonucleic Acid Polymerases in Virions of Rous Sarcoma Virus and in Rous Sarcoma Virus-Infected Chicken Cells

McArdle Laboratory, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Labeled virions of Rous sarcoma virus (RSV) were disrupted with detergent and analyzed on equilibrium sucrose density gradients. A core fraction at a density of approximately 1.24 g/cc contained all of the ³H-uridine label and about 30% of the ³H-leucine label from the virions. Endogenous viral deoxyribonucleic acid (DNA) polymerase activity was only found in the same location. Additional ribonucleic acid (RNA)- and DNA-dependent DNA polymerase activities were found at the top of the gradients. RNA-dependent and DNA-dependent DNA polymerase activities were also found in RSV-converted chicken cells. Particles containing these activities were released from cells by detergent and were shown to contain viral RNA. These particles were analyzed on equilibrium sucrose density gradients and were found to have densities different from virion cores.

This article has been cited by other articles:

• Levinson, W. E., Varmus, H. E., Garapin, A.-C., Bishop, J. M. (1972). DNA of Rous Sarcoma Virus: Its Nature and Significance. Science 175: 76-78 [Abstract]