This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Warner, H. R. Search for Related Content PubMed PubMed Citation Articles by Warner, H. R.

Partial Suppression of Bacteriophage T4 Ligase Mutations by T4 Endonuclease II Deficiency: Role of Host Ligase ¹

^a Department of Biochemistry, College of Biological Sciences, University of Minnesota, St. Paul, Minnesota 55101

ABSTRACT

Endonuclease II-deficient, ligase-deficient double mutants of phage T4 induce considerably more deoxyribonucleic acid (DNA) synthesis after infection of Escherichia coli B than does the ligase-deficient single mutant. Furthermore, the double mutant can replicate 10 to 15% as well as wild-type T4, whereas the single mutant fails to replicate. When the E. coli host is also deficient in ligase, the double mutant resembles the single mutant. The results indicate that host ligase can substitute for phage ligase when the host DNA is not attacked by the phage-induced endonuclease II.

² Recipient of Public Health Service Research Career Development Award GM 45729 from the National Institute of General Medical Sciences.

¹ This publication is Scientific Paper no. 7443 of the Minnesota Agricultural Experiment Station.

This article has been cited by other articles:

• Miller, E. S., Kutter, E., Mosig, G., Arisaka, F., Kunisawa, T., Ruger, W. (2003). Bacteriophage T4 Genome. Microbiol. Mol. Biol. Rev. 67: 86-156 [Abstract] [Full Text]