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J Virol. 1971 April; 7(4): 515-523
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Abortive Infection of Sporulating Bacillus subtilis 168 by 2 Bacteriophage

Department of Microbiology, Scripps Clinic and Research Foundation, La Jolla, California 92037

ABSTRACT

Bacteriophage 2 is unable to replicate in Bacillus subtilis 168. Although some phage deoxyribonucleic acid (DNA) synthesis can occur, the DNA made is not biologically active and sedimentation analysis reveals that it is smaller in size than that of mature DNA or DNA isolated from 2-infected permissive hosts. Messenger ribonucleic acid hybridizable with 2 DNA is also synthesized in 2-infected cells of 168. Mutants of 168 which are permissive hosts for 2 have been isolated. These mutants are defective in sporulation and possess the phenotype of "early sporulation mutants." The majority map in two locations, one near the lys locus opposite the trp locus (spoA locus) and the other tightly linked to a phe locus.