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J Virol. 1971 March; 7(3): 389-394
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Ribonucleic Acud Polymerase in Virions of Newcastle Disease Virus: Comparison with the Vesicular Stomatitis Virus Polymerase

Alice S. Huang1, David Baltimore and Michael A. Bratt

Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

ABSTRACT

The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into ribonucleic acid (RNA). Optimal conditions for this polymerase activity were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C. However, the specific activity of the NDV polymerase was less than 3% that of the VSV polymerase. Product RNA species from the NDV and VSV polymerase reactions annealed specifically to the homologous virion RNA species. Transcriptive intermediates containing product RNA attached to the respective virion RNA could be identified in both systems.

FOOTNOTES

1 Present address: Channing Laboratory, 774 Albany St., Boston, Mass. 02118.

J Virol. 1971 March; 7(3): 389-394
Copyright © 1971 American Society for Microbiology. All Rights Reserved.



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