This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Witmer, H. Articles by Fraser, D. Search for Related Content PubMed PubMed Citation Articles by Witmer, H. Articles by Fraser, D.

 Previous Article  |  Next Article 

J Virol. 1971 March; 7(3): 323-331
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Photodynamic Action of Proflavine on Coliphage T3 III. Damages to the Deoxyribonucleic Acid Associated with Rx1 and Rx2

¹ Microbiology Department, Indiana University, Bloomington, Indiana 47401

ABSTRACT

Ultracentrifugational studies with deoxyribonucleic acid (DNA) extracted from phage exposed to light in the presence of either 0.25 or 8.5 µg of proflavine per ml reveal that the lethal damage of Rx1 renders DNA alkali-labile, with lethality resulting from damage that occurs singly on either strand or simultaneously on both strands. Apparently nonlethal damages temporally associated with Rx1 consist of (i) heat and alkali-labile cross-links (which produce undenatured DNA that migrates at 45S) and (ii) heat-labile bonds. The formation of 45S material is a linear function of light dose, and the production of this material ceases when Rx2 appears at the higher dye concentration. No tendency to plateau is seen at 0.25 µg of dye per ml. The nature of the lethal damage of Rx2 could not be determined. Damages that were temporally associated with Rx2 at 8.5 µg of dye per ml were heat-labile, alkali-stable cross-links (undenatured DNA, 38 to 41S; alkali-denatured-reneutralized DNA, 85 to 89S) and some double-stranded breaks. No such changes were seen at 0.25 µg of dye per ml.