This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Manickan, E. Articles by Rouse, B. T. Search for Related Content PubMed PubMed Citation Articles by Manickan, E. Articles by Rouse, B. T.

 Previous Article  |  Next Article 

J. Virol., 08 1995, 4711-4716, Vol 69, No. 8
Copyright © 1995, American Society for Microbiology

Vaccination with recombinant vaccinia viruses expressing ICP27 induces protective immunity against herpes simplex virus through CD4+ Th1+ T cells

E Manickan, M Francotte, N Kuklin, M Dewerchin, C Molitor, D Gheysen, M Slaoui and BT Rouse
Department of Microbiology, College of Veterinary Medicine, University of Tennessee, Knoxville 37996-0845, USA.

This study was designed to evaluate the efficacy and mechanisms of protection mediated by recombinant vaccinia viruses encoding immediate- early (IE) proteins of herpes simplex virus type 2 (HSV-2). Three mouse strains were immunized against the IE proteins ICP27, ICP0, and ICP4, and mice were challenged intracutaneously in the zosteriform model with HSV-2 strain MS. Protection was observed only following immunization with the ICP27 construct and then only in the BALB/c mouse strain. Protection in BALB/c mice was ablated by CD4+ T-cell suppression but remained intact in animals depleted of CD8+ T cells. Moreover, protection could be afforded to SCID nude recipients with CD4+ but not CD8+ T cells from ICP27-immunized mice. Only BALB/c mice developed a delayed-type hypersensitivity reaction to HSV-2, and in vitro measurements of humoral and cell-mediated immunity revealed response patterns to ICP27 and HSV that differed between protected BALB/c and unprotected mouse strains. Accordingly, BALB/c responses showed antigen- induced cytokine profiles dominated by type 1 cytokines, whereas C57BL/6 and C3H/HeN mice generated cytokine responses mainly of the type 2 variety. Our results may indicate that protection against zosterification is mainly mediated by CD4+ T cells that express a type 1 cytokine profile and that protective vaccines against HSV which effectively induce such T-cell responses should be chosen.

This article has been cited by other articles:

• van Lint, A., Ayers, M., Brooks, A. G., Coles, R. M., Heath, W. R., Carbone, F. R. (2004). Herpes Simplex Virus-Specific CD8+ T Cells Can Clear Established Lytic Infections from Skin and Nerves and Can Partially Limit the Early Spread of Virus after Cutaneous Inoculation. J. Immunol. 172: 392-397 [Abstract] [Full Text]  
• Koelle, D. M., Liu, Z., McClurkan, C. L., Cevallos, R. C., Vieira, J., Hosken, N. A., Meseda, C. A., Snow, D. C., Wald, A., Corey, L. (2003). Immunodominance among herpes simplex virus-specific CD8 T cells expressing a tissue-specific homing receptor. Proc. Natl. Acad. Sci. USA 100: 12899-12904 [Abstract] [Full Text]  
• Koelle, D. M., Corey, L. (2003). Recent Progress in Herpes Simplex Virus Immunobiology and Vaccine Research. Clin. Microbiol. Rev. 16: 96-113 [Abstract] [Full Text]  
• McNeal, M. M., VanCott, J. L., Choi, A. H. C., Basu, M., Flint, J. A., Stone, S. C., Clements, J. D., Ward, R. L. (2002). CD4 T Cells Are the Only Lymphocytes Needed To Protect Mice against Rotavirus Shedding after Intranasal Immunization with a Chimeric VP6 Protein and the Adjuvant LT(R192G). J. Virol. 76: 560-568 [Abstract] [Full Text]  
• Koelle, D. M., Chen, H. B., Gavin, M. A., Wald, A., Kwok, W. W., Corey, L. (2001). CD8 CTL from Genital Herpes Simplex Lesions: Recognition of Viral Tegument and Immediate Early Proteins and Lysis of Infected Cutaneous Cells. J. Immunol. 166: 4049-4058 [Abstract] [Full Text]  
• Maloy, K. J., Burkhart, C., Junt, T. M., Odermatt, B., Oxenius, A., Piali, L., Zinkernagel, R. M., Hengartner, H. (2000). CD4+ T Cell Subsets during Virus Infection: Protective Capacity Depends on Effector Cytokine Secretion and on Migratory Capability. J. Exp. Med. 191: 2159-2170 [Abstract] [Full Text]  
• Mikloska, Z., Ruckholdt, M., Ghadiminejad, I., Dunckley, H., Denis, M., Cunningham, A. L. (2000). Monophosphoryl Lipid A and QS21 Increase CD8 T Lymphocyte Cytotoxicity to Herpes Simplex Virus-2 Infected Cell Proteins 4 and 27 Through IFN-{gamma} and IL-12 Production. J. Immunol. 164: 5167-5176 [Abstract] [Full Text]  
• Blaney, J. E. Jr., Nobusawa, E., Brehm, M. A., Bonneau, R. H., Mylin, L. M., Fu, T.-M., Kawaoka, Y., Tevethia, S. S. (1998). Immunization with a Single Major Histocompatibility Complex Class I-Restricted Cytotoxic T-Lymphocyte Recognition Epitope of Herpes Simplex Virus Type 2 Confers Protective Immunity. J. Virol. 72: 9567-9574 [Abstract] [Full Text]  
• Chun, S., Daheshia, M., Kuklin, N. A., Rouse, B. T. (1998). Modulation of Viral Immunoinflammatory Responses with Cytokine DNA Administered by Different Routes. J. Virol. 72: 5545-5551 [Abstract] [Full Text]