Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication

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J. Virol., 05 1995, 2729-2736, Vol 69, No. 5
Copyright © 1995, American Society for Microbiology

Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication

A Engelman, G Englund, JM Orenstein, MA Martin and R Craigie
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892, USA.

The integration of a DNA copy of the human immunodeficiency virus type 1 (HIV-1) genome into a chromosome of an infected cell is a pivotal step in virus replication. Integration requires the activity of the virus-encoded integrase, which enters the cell as a component of the virion. Results of numerous mutagenesis studies have identified amino acid residues and protein domains of HIV-1 integrase critical for in vitro activity, but only a few of these mutants have been studied for their effects on HIV replication. We have introduced site-directed changes into an infectious DNA clone of HIV-1 and show that integrase mutations can affect virus replication at a variety of steps. We identified mutations that altered virion morphology, levels of particle- associated integrase and reverse transcriptase, and viral DNA synthesis. One replication-defective mutant virus which had normal morphology and protein composition displayed increased levels of circular viral DNA following infection of a T-cell line. This virus also had a significant titer in a CD4-positive indicator cell assay, which requires the viral Tat protein. Although unintegrated viral DNA can serve as a template for Tat expression in infected indicator cells, this level of expression is insufficient to support a spreading viral infection in CD4-positive lymphocytes.

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Holmes-Son, M. L., Chow, S. A. (2000). Integrase-LexA Fusion Proteins Incorporated into Human Immunodeficiency Virus Type 1 That Contains a Catalytically Inactive Integrase Gene Are Functional To Mediate Integration. J. Virol. 74: 11548-11556 [Abstract] [Full Text]
Guo, J., Wu, T., Anderson, J., Kane, B. F., Johnson, D. G., Gorelick, R. J., Henderson, L. E., Levin, J. G. (2000). Zinc Finger Structures in the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Facilitate Efficient Minus- and Plus-Strand Transfer. J. Virol. 74: 8980-8988 [Abstract] [Full Text]
Petit, C., Schwartz, O., Mammano, F. (2000). The Karyophilic Properties of Human Immunodeficiency Virus Type 1 Integrase Are Not Required for Nuclear Import of Proviral DNA. J. Virol. 74: 7119-7126 [Abstract] [Full Text]
Tsurutani, N., Kubo, M., Maeda, Y., Ohashi, T., Yamamoto, N., Kannagi, M., Masuda, T. (2000). Identification of Critical Amino Acid Residues in Human Immunodeficiency Virus Type 1 IN Required for Efficient Proviral DNA Formation at Steps prior to Integration in Dividing and Nondividing Cells. J. Virol. 74: 4795-4806 [Abstract] [Full Text]
Cimarelli, A., Sandin, S., Höglund, S., Luban, J. (2000). Rescue of Multiple Viral Functions by a Second-Site Suppressor of a Human Immunodeficiency Virus Type 1 Nucleocapsid Mutation. J. Virol. 74: 4273-4283 [Abstract] [Full Text]
Hindmarsh, P., Leis, J. (1999). Retroviral DNA Integration. Microbiol. Mol. Biol. Rev. 63: 836-843 [Abstract] [Full Text]
Brown, H. E. V., Chen, H., Engelman, A. (1999). Structure-Based Mutagenesis of the Human Immunodeficiency Virus Type 1 DNA Attachment Site: Effects on Integration and cDNA Synthesis. J. Virol. 73: 9011-9020 [Abstract] [Full Text]
Gorelick, R. J., Fu, W., Gagliardi, T. D., Bosche, W. J., Rein, A., Henderson, L. E., Arthur, L. O. (1999). Characterization of the Block in Replication of Nucleocapsid Protein Zinc Finger Mutants from Moloney Murine Leukemia Virus. J. Virol. 73: 8185-8195 [Abstract] [Full Text]
Liu, H., Wu, X., Xiao, H., Kappes, J. C. (1999). Targeting Human Immunodeficiency Virus (HIV) Type 2 Integrase Protein into HIV Type 1. J. Virol. 73: 8831-8836 [Abstract] [Full Text]
Chen, H., Wei, S.-Q., Engelman, A. (1999). Multiple Integrase Functions Are Required to Form the Native Structure of the Human Immunodeficiency Virus Type I Intasome. J. Biol. Chem. 274: 17358-17364 [Abstract] [Full Text]
Dettenhofer, M., Yu, X.-F. (1999). Proline Residues in Human Immunodeficiency Virus Type 1�p6Gag Exert a Cell Type-Dependent Effect on Viral Replication and Virion Incorporation of Pol Proteins. J. Virol. 73: 4696-4704 [Abstract] [Full Text]
Kiernan, R. E., Ono, A., Freed, E. O. (1999). Reversion of a Human Immunodeficiency Virus Type 1�Matrix Mutation Affecting Gag Membrane Binding, Endogenous Reverse Transcriptase Activity, and Virus Infectivity. J. Virol. 73: 4728-4737 [Abstract] [Full Text]
Ishikawa, T., Okui, N., Kobayashi, N., Sakuma, R., Kitamura, T., Kitamura, Y. (1999). Monoclonal Antibodies against the Minimal DNA-Binding Domain in the Carboxyl-Terminal Region of Human Immunodeficiency Virus Type 1�Integrase. J. Virol. 73: 4475-4480 [Abstract] [Full Text]
Yang, F., Leon, O., Greenfield, N. J., Roth, M. J. (1999). Functional Interactions of the HHCC Domain of Moloney Murine Leukemia Virus Integrase Revealed by Nonoverlapping Complementation and Zinc-Dependent Dimerization. J. Virol. 73: 1809-1817 [Abstract] [Full Text]
Wu, X., Liu, H., Xiao, H., Conway, J. A., Hehl, E., Kalpana, G. V., Prasad, V., Kappes, J. C. (1999). Human Immunodeficiency Virus Type 1�Integrase Protein Promotes Reverse Transcription through Specific Interactions with the Nucleoprotein Reverse Transcription Complex. J. Virol. 73: 2126-2135 [Abstract] [Full Text]
Nymark-McMahon, M. H., Sandmeyer, S. B. (1999). Mutations in Nonconserved Domains of Ty3 Integrase Affect Multiple Stages of the Ty3 Life Cycle. J. Virol. 73: 453-465 [Abstract] [Full Text]
King, P. J., Robinson, W. E. Jr. (1998). Resistance to the Anti-Human Immunodeficiency Virus Type 1�Compound L-Chicoric Acid Results from a Single Mutation at Amino Acid 140�of Integrase. J. Virol. 72: 8420-8424 [Abstract] [Full Text]
Kiernan, R. E., Ono, A., Englund, G., Freed, E. O. (1998). Role of Matrix in an Early Postentry Step in the Human Immunodeficiency Virus Type 1�Life Cycle. J. Virol. 72: 4116-4126 [Abstract] [Full Text]
Dildine, S. L., Respess, J., Jolly, D., Sandmeyer, S. B. (1998). A Chimeric Ty3/Moloney Murine Leukemia Virus Integrase Protein Is Active In Vivo. J. Virol. 72: 4297-4307 [Abstract] [Full Text]
Drake, R. R., Neamati, N., Hong, H., Pilon, A. A., Sunthankar, P., Hume, S. D., Milne, G. W.�A., Pommier, Y. (1998). Identification of a nucleotide binding site in HIV-1 integrase. Proc. Natl. Acad. Sci. USA 95: 4170-4175 [Abstract] [Full Text]
Yu, X.-F., Dawson, L., Tian, C.-J., Flexner, C., Dettenhofer, M. (1998). Mutations of the Human Immunodeficiency Virus Type 1�p6Gag Domain Result in Reduced Retention of Pol Proteins during Virus Assembly. J. Virol. 72: 3412-3417 [Abstract] [Full Text]
Acel, A., Udashkin, B. E., Wainberg, M. A., Faust, E. A. (1998). Efficient Gap Repair Catalyzed In Vitro by an Intrinsic DNA Polymerase Activity of Human Immunodeficiency Virus Type 1�Integrase. J. Virol. 72: 2062-2071 [Abstract] [Full Text]
Scottoline, B P, Chow, S, Ellison, V, Brown, P O (1997). Disruption of the terminal base pairs of retroviral DNA during integration.. Genes Dev. 11: 371-382 [Abstract]
Jenkins, T. M., Engelman, A., Ghirlando, R., Craigie, R. (1996). A Soluble Active Mutant of HIV-1 Integrase. J. Biol. Chem. 271: 7712-7718 [Abstract] [Full Text]
Cara, A., Cereseto, A., Lori, F., Reitz, M. S. Jr. (1996). HIV-1 Protein Expression from Synthetic Circles of DNA Mimicking the Extrachromosomal Forms of Viral DNA. J. Biol. Chem. 271: 5393-5397 [Abstract] [Full Text]

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