This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neumann, M.
Right arrow Articles by Brack- Werner, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neumann, M.
Right arrow Articles by Brack- Werner, R.

 Previous Article  |  Next Article 

J. Virol., Apr 1995, 2159-2167, Vol 69, No. 4
Copyright © 1995, American Society for Microbiology

Restriction of human immunodeficiency virus type 1 production in a human astrocytoma cell line is associated with a cellular block in Rev function

M Neumann, BK Felber, A Kleinschmidt, B Froese, V Erfle, GN Pavlakis and R Brack- Werner
GSF-Institute of Molecular Virology, Oberschleissheim, Germany.

Chronically human immunodeficiency virus type 1 (HIV-1) strain IIIB- infected human TH4-7-5 astrocytoma cells show low-level virus production. Cocultivation of TH4-7-5 cells with myelomonocytic cells led to active virus production in these target cells after a lag period, indicating cell-determined restriction of virus replication in the glial cells. HIV-1 transcript patterns of TH4-7-5 cells contained only a small proportion of Rev-dependent mRNA species, mimicking a Rev- negative phenotype despite the presence of rev mRNAs and protein. Sequencing of the single provirus integrated in TH4-7-5 cells demonstrated that the rev gene and the Rev-responsive element are intact. These results suggested inhibited function of the Rev- regulatory unit in these astrocytoma cells. Transfection of TH4-7-5 cells with a Rev expression plasmid resulted in weak or no induction of proviral p24gag antigen levels compared with the dramatic increase observed in Rev-permissive HeLa cells. Immunofluorescence analysis of TH4-7-5 cells transfected with a rev-expressing plasmid revealed prominent cytoplasmic and nuclear-nucleolar localization of Rev, in contrast to the predominant nuclear-nucleolar localization pattern of Rev in HeLa cells. We conclude that restriction of virus production in TH4-7-5 cells is at least partially due to a block in Rev-dependent posttranscriptional regulation of HIV expression.


This article has been cited by other articles:

  • Haedicke, J., Brown, C., Naghavi, M. H. (2009). The brain-specific factor FEZ1 is a determinant of neuronal susceptibility to HIV-1 infection. Proc. Natl. Acad. Sci. USA 106: 14040-14045 [Abstract] [Full Text]  
  • Carroll-Anzinger, D., Kumar, A., Adarichev, V., Kashanchi, F., Al-Harthi, L. (2007). Human Immunodeficiency Virus-Restricted Replication in Astrocytes and the Ability of Gamma Interferon To Modulate This Restriction Are Regulated by a Downstream Effector of the Wnt Signaling Pathway. J. Virol. 81: 5864-5871 [Abstract] [Full Text]  
  • Kaniowska, D., Kaminski, R., Amini, S., Radhakrishnan, S., Rappaport, J., Johnson, E., Khalili, K., Del Valle, L., Darbinyan, A. (2006). Cross-Interaction between JC Virus Agnoprotein and Human Immunodeficiency Virus Type 1 (HIV-1) Tat Modulates Transcription of the HIV-1 Long Terminal Repeat in Glial Cells.. J. Virol. 80: 9288-9299 [Abstract] [Full Text]  
  • Carroll-Anzinger, D., Al-Harthi, L. (2006). Gamma Interferon Primes Productive Human Immunodeficiency Virus Infection in Astrocytes. J. Virol. 80: 541-544 [Abstract] [Full Text]  
  • Ong, C. L., Thorpe, J. C., Gorry, P. R., Bannwarth, S., Jaworowski, A., Howard, J. L., Chung, S., Campbell, S., Christensen, H. S., Clerzius, G., Mouland, A. J., Gatignol, A., Purcell, D. F. J. (2005). Low TRBP Levels Support an Innate Human Immunodeficiency Virus Type 1 Resistance in Astrocytes by Enhancing the PKR Antiviral Response. J. Virol. 79: 12763-12772 [Abstract] [Full Text]  
  • Overholser, E. D., Coleman, G. D., Bennett, J. L., Casaday, R. J., Zink, M. C., Barber, S. A., Clements, J. E. (2003). Expression of Simian Immunodeficiency Virus (SIV) Nef in Astrocytes during Acute and Terminal Infection and Requirement of Nef for Optimal Replication of Neurovirulent SIV In Vitro. J. Virol. 77: 6855-6866 [Abstract] [Full Text]  
  • Li, J., Liu, Y., Park, I.-W., He, J. J. (2002). Expression of Exogenous Sam68, the 68-Kilodalton Src-Associated Protein in Mitosis, Is Able To Alleviate Impaired Rev Function in Astrocytes. J. Virol. 76: 4526-4535 [Abstract] [Full Text]  
  • Canki, M., Thai, J. N. F., Chao, W., Ghorpade, A., Potash, M. J., Volsky, D. J. (2001). Highly Productive Infection with Pseudotyped Human Immunodeficiency Virus Type 1 (HIV-1) Indicates No Intracellular Restrictions to HIV-1 Replication in Primary Human Astrocytes. J. Virol. 75: 7925-7933 [Abstract] [Full Text]  
  • Neumann, M, Afonina, E, Ceccherini-Silberstein, F, Schlicht, S, Erfle, V, Pavlakis, G., Brack-Werner, R (2001). Nucleocytoplasmic transport in human astrocytes: decreased nuclear uptake of the HIV Rev shuttle protein. J. Cell Sci. 114: 1717-1729 [Abstract]  
  • Aquaro, S., Panti, S., Caroleo, M. C., Balestra, E., Cenci, A., Forbici, F., Ippolito, G., Mastino, A., Testi, R., Mollace, V., Calio, R., Perno, C. F. (2000). Primary macrophages infected by human immunodeficiency virus trigger CD95-mediated apoptosis of uninfected astrocytes. J. Leukoc. Biol. 68: 429-435 [Abstract] [Full Text]  
  • Ludwig, E., Silberstein, F. C., van Empel, J., Erfle, V., Neumann, M., Brack-Werner, R. (1999). Diminished Rev-Mediated Stimulation of Human Immunodeficiency Virus Type 1 Protein Synthesis Is a Hallmark of Human Astrocytes. J. Virol. 73: 8279-8289 [Abstract] [Full Text]  
  • Koedel, U., Kohleisen, B., Sporer, B., Lahrtz, F., Ovod, V., Fontana, A., Erfle, V., Pfister, H.-W. (1999). HIV Type 1 Nef Protein Is a Viral Factor for Leukocyte Recruitment into the Central Nervous System. J. Immunol. 163: 1237-1245 [Abstract] [Full Text]  
  • Gorry, P. R., Howard, J. L., Churchill, M. J., Anderson, J. L., Cunningham, A., Adrian, D., McPhee, D. A., Purcell, D. F. J. (1999). Diminished Production of Human Immunodeficiency Virus Type 1 in Astrocytes Results from Inefficient Translation of gag, env, and nef mRNAs despite Efficient Expression of Tat and Rev. J. Virol. 73: 352-361 [Abstract] [Full Text]  
  • He, J., deCastro, C. M., Vandenbark, G. R., Busciglio, J., Gabuzda, D. (1997). Astrocyte apoptosis induced by HIV-1 transactivation of the c-kit protooncogene. Proc. Natl. Acad. Sci. USA 94: 3954-3959 [Abstract] [Full Text]  
  • Afonina, E., Neumann, M., Pavlakis, G. N. (1997). Preferential Binding of Poly(A)-binding Protein 1to an Inhibitory RNA Element in the Human Immunodeficiency Virus Type 1gag mRNA. J. Biol. Chem. 272: 2307-2311 [Abstract] [Full Text]  
  • Bannwarth, S., Talakoub, L., Letourneur, F., Duarte, M., Purcell, D. F., Hiscott, J., Gatignol, A. (2001). Organization of the Human tarbp2 Gene Reveals Two Promoters That Are Repressed in an Astrocytic Cell Line. J. Biol. Chem. 276: 48803-48813 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»