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J. Virol., 12 1995, 8057-8060, Vol 69, No. 12
Copyright © 1995, American Society for Microbiology

Binding motifs predict major histocompatibility complex class II- restricted epitopes in the Sendai virus M protein

GA Cole, T Tao, TL Hogg, KW Ryan and DL Woodland
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.

Major histocompatibility complex (MHC) class I ligand motifs have been defined for a number of class I molecules and have been successfully used to identify class I-restricted cytotoxic T-cell epitopes. In contrast, the relative degeneracy of sequence motifs in naturally processed MHC class II ligands has suggested that they may be of more limited use. Here, we use a predicted I-Ab ligand motif to identify antigenic peptides in the Sendai virus Enders strain matrix (M) protein. The entire coding sequence of the M protein was derived, and seven peptide sequences that contained the predicted I-Ab motif were identified. Analysis of I-Ab-restricted M-specific T-cell hybridomas for reactivity to these synthetic peptides identified two distinct epitopes. These data demonstrate that MHC class II motifs can be valuable in predicting T-cell epitopes.

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