This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lu, Y. L.
Right arrow Articles by Ratner, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lu, Y. L.
Right arrow Articles by Ratner, L.

 Previous Article  |  Next Article 

J. Virol., Nov 1995, 6873-6879, Vol 69, No. 11
Copyright © 1995, American Society for Microbiology

A leucine triplet repeat sequence (LXX)4 in p6gag is important for Vpr incorporation into human immunodeficiency virus type 1 particles

YL Lu, RP Bennett, JW Wills, R Gorelick and L Ratner
Department of Medicine, Washington University, St. Louis, Missouri 63110, USA.

Incorporation of Vpr into human immunodeficiency virus type 1 (HIV-1) virions is mediated by the Gag protein, independently of other viral components. We have coexpressed Vpr and Gag constructs in a vaccinia virus expression system in order to map the region of Gag involved in Vpr packaging. Deletion of the carboxyl-terminal p6 region of Gag impaired the ability of Gag to package Vpr. To confirm the role of p6 in Vpr packaging, Rous sarcoma virus (RSV)-HIV chimeras containing HIV- 1 p6 were constructed. Although RSV Gag does not package Vpr into virus particles, a chimera containing HIV-1 p6 is sufficient for Vpr incorporation. To map the region of p6 involved in Vpr packaging, a series of p6 point mutations and deletion mutations was analyzed. Mutations in the N-terminal p6 proline-rich domain, for which preliminary evidence shows a marked decrease in virion incorporated RNA, did not affect Vpr incorporation. Deletion of residues 1 to 31 of HIV-1 p6 did not affect Vpr packaging, but residues 35 to 47, including an (LXX)4 domain, were required for Vpr incorporation into virus particles.


This article has been cited by other articles:

  • Arokium, H., Kamata, M., Chen, I. (2009). Virion-Associated Vpr of Human Immunodeficiency Virus Type 1 Triggers Activation of Apoptotic Events and Enhances Fas-Induced Apoptosis in Human T Cells. J. Virol. 83: 11283-11297 [Abstract] [Full Text]  
  • Kamata, M., Watanabe, N., Nagaoka, Y., Chen, I. S. Y. (2008). Human Immunodeficiency Virus Type 1 Vpr Binds to the N Lobe of the Wee1 Kinase Domain and Enhances Kinase Activity for Cdc2. J. Virol. 82: 5672-5682 [Abstract] [Full Text]  
  • Votteler, J., Studtrucker, N., Sorgel, S., Munch, J., Rucker, E., Kirchhoff, F., Schick, B., Henklein, P., Fossen, T., Bruns, K., Sharma, A., Wray, V., Schubert, U. (2007). Proline 35 of Human Immunodeficiency Virus Type 1 (HIV-1) Vpr Regulates the Integrity of the N-Terminal Helix and the Incorporation of Vpr into Virus Particles and Supports the Replication of R5-Tropic HIV-1 in Human Lymphoid Tissue Ex Vivo. J. Virol. 81: 9572-9576 [Abstract] [Full Text]  
  • Tan, W., Dong, Z., Wilkinson, T. A., Barbas, C. F. III, Chow, S. A. (2006). Human Immunodeficiency Virus Type 1 Incorporated with Fusion Proteins Consisting of Integrase and the Designed Polydactyl Zinc Finger Protein E2C Can Bias Integration of Viral DNA into a Predetermined Chromosomal Region in Human Cells. J. Virol. 80: 1939-1948 [Abstract] [Full Text]  
  • Fossen, T., Wray, V., Bruns, K., Rachmat, J., Henklein, P., Tessmer, U., Maczurek, A., Klinger, P., Schubert, U. (2005). Solution Structure of the Human Immunodeficiency Virus Type 1 p6 Protein. J. Biol. Chem. 280: 42515-42527 [Abstract] [Full Text]  
  • Varadarajan, P., Mahalingam, S., Liu, P., Ng, S. B. H., Gandotra, S., Dorairajoo, D. S. K., Balasundaram, D. (2005). The Functionally Conserved Nucleoporins Nup124p from Fission Yeast and the Human Nup153 Mediate Nuclear Import and Activity of the Tf1 Retrotransposon and HIV-1 Vpr. Mol. Biol. Cell 16: 1823-1838 [Abstract] [Full Text]  
  • Bleiber, G., Peters, S., Martinez, R., Cmarko, D., Meylan, P., Telenti, A. (2004). The central region of human immunodeficiency virus type 1 p6 protein (Gag residues S14-I31) is dispensable for the virus in vitro. J. Gen. Virol. 85: 921-927 [Abstract] [Full Text]  
  • Ramanathan, M. P., Curley, E. III, Su, M., Chambers, J. A., Weiner, D. B. (2002). Carboxyl Terminus of hVIP/mov34 Is Critical for HIV-1-Vpr Interaction and Glucocorticoid-mediated Signaling. J. Biol. Chem. 277: 47854-47860 [Abstract] [Full Text]  
  • Jenkins, Y., Pornillos, O., Rich, R. L., Myszka, D. G., Sundquist, W. I., Malim, M. H. (2001). Biochemical Analyses of the Interactions between Human Immunodeficiency Virus Type 1 Vpr and p6Gag. J. Virol. 75: 10537-10542 [Abstract] [Full Text]  
  • Chen, C., Li, F., Montelaro, R. C. (2001). Functional Roles of Equine Infectious Anemia Virus Gag p9 in Viral Budding and Infection. J. Virol. 75: 9762-9770 [Abstract] [Full Text]  
  • Jenkins, Y., Sanchez, P. V., Meyer, B. E., Malim, M. H. (2001). Nuclear Export of Human Immunodeficiency Virus Type 1 Vpr Is Not Required for Virion Packaging. J. Virol. 75: 8348-8352 [Abstract] [Full Text]  
  • Mahalingam, S., Van Tine, B., Santiago, M. L., Gao, F., Shaw, G. M., Hahn, B. H. (2001). Functional Analysis of the Simian Immunodeficiency Virus Vpx Protein: Identification of Packaging Determinants and a Novel Nuclear Targeting Domain. J. Virol. 75: 362-374 [Abstract] [Full Text]  
  • Holmes-Son, M. L., Chow, S. A. (2000). Integrase-LexA Fusion Proteins Incorporated into Human Immunodeficiency Virus Type 1 That Contains a Catalytically Inactive Integrase Gene Are Functional To Mediate Integration. J. Virol. 74: 11548-11556 [Abstract] [Full Text]  
  • Müller, B., Tessmer, U., Schubert, U., Kräusslich, H.-G. (2000). Human Immunodeficiency Virus Type 1 Vpr Protein Is Incorporated into the Virion in Significantly Smaller Amounts than Gag and Is Phosphorylated in Infected Cells. J. Virol. 74: 9727-9731 [Abstract] [Full Text]  
  • Sherman, M. P., de Noronha, C. M. C., Pearce, D., Greene, W. C. (2000). Human Immunodeficiency Virus Type 1 Vpr Contains Two Leucine-Rich Helices That Mediate Glucocorticoid Receptor Coactivation Independently of Its Effects on G2 Cell Cycle Arrest. J. Virol. 74: 8159-8165 [Abstract] [Full Text]  
  • Zhou, Y., Ratner, L. (2000). Phosphorylation of Human Immunodeficiency Virus Type 1 Vpr Regulates Cell Cycle Arrest. J. Virol. 74: 6520-6527 [Abstract] [Full Text]  
  • Nishizawa, M., Kamata, M., Katsumata, R., Aida, Y. (2000). A Carboxy-Terminally Truncated Form of the Human Immunodeficiency Virus Type 1 Vpr Protein Induces Apoptosis via G1 Cell Cycle Arrest. J. Virol. 74: 6058-6067 [Abstract] [Full Text]  
  • Pancio, H. A., Vander Heyden, N., Ratner, L. (2000). The C-Terminal Proline-Rich Tail of Human Immunodeficiency Virus Type 2 Vpx Is Necessary for Nuclear Localization of the Viral Preintegration Complex in Nondividing Cells. J. Virol. 74: 6162-6167 [Abstract] [Full Text]  
  • Accola, M. A., Öhagen, A., Göttlinger, H. G. (2000). Isolation of Human Immunodeficiency Virus Type 1 Cores: Retention of Vpr in the Absence of p6gag. J. Virol. 74: 6198-6202 [Abstract] [Full Text]  
  • Accola, M. A., Strack, B., Göttlinger, H. G. (2000). Efficient Particle Production by Minimal Gag Constructs Which Retain the Carboxy-Terminal Domain of Human Immunodeficiency Virus Type 1 Capsid-p2 and a Late Assembly Domain. J. Virol. 74: 5395-5402 [Abstract] [Full Text]  
  • Sei, S., Yang, Q.-e., O'Neill, D., Yoshimura, K., Nagashima, K., Mitsuya, H. (2000). Identification of a Key Target Sequence To Block Human Immunodeficiency Virus Type 1 Replication within the gag-pol Transframe Domain. J. Virol. 74: 4621-4633 [Abstract] [Full Text]  
  • Alexander, L., Weiskopf, E., Greenough, T. C., Gaddis, N. C., Auerbach, M. R., Malim, M. H., O'Brien, S. J., Walker, B. D., Sullivan, J. L., Desrosiers, R. C. (2000). Unusual Polymorphisms in Human Immunodeficiency Virus Type 1 Associated with Nonprogressive Infection. J. Virol. 74: 4361-4376 [Abstract] [Full Text]  
  • Stewart, S. A., Poon, B., Song, J. Y., Chen, I. S. Y. (2000). Human Immunodeficiency Virus Type 1 Vpr Induces Apoptosis through Caspase Activation. J. Virol. 74: 3105-3111 [Abstract] [Full Text]  
  • Accola, M. A., Bukovsky, A. A., Jones, M. S., Göttlinger, H. G. (1999). A Conserved Dileucine-Containing Motif in p6gag Governs the Particle Association of Vpx and Vpr of Simian Immunodeficiency Viruses SIVmac and SIVagm. J. Virol. 73: 9992-9999 [Abstract] [Full Text]  
  • Stewart, S. A., Poon, B., Jowett, J. B. M., Xie, Y., Chen, I. S. Y. (1999). Lentiviral delivery of HIV-1 Vpr protein induces apoptosis in transformed cells. Proc. Natl. Acad. Sci. USA 96: 12039-12043 [Abstract] [Full Text]  
  • Dettenhofer, M., Yu, X.-F. (1999). Proline Residues in Human Immunodeficiency Virus Type 1 p6Gag Exert a Cell Type-Dependent Effect on Viral Replication and Virion Incorporation of Pol Proteins. J. Virol. 73: 4696-4704 [Abstract] [Full Text]  
  • Bachand, F., Yao, X.-J., Hrimech, M., Rougeau, N., Cohen, E. A. (1999). Incorporation of Vpr into Human Immunodeficiency Virus Type 1 Requires a Direct Interaction with the p6 Domain of the p55 Gag Precursor. J. Biol. Chem. 274: 9083-9091 [Abstract] [Full Text]  
  • Dettenhofer, M., Yu, X.-F. (1999). Highly Purified Human Immunodeficiency Virus Type 1 Reveals a Virtual Absence of Vif in Virions. J. Virol. 73: 1460-1467 [Abstract] [Full Text]  
  • Ott, D. E., Chertova, E. N., Busch, L. K., Coren, L. V., Gagliardi, T. D., Johnson, D. G. (1999). Mutational Analysis of the Hydrophobic Tail of the Human Immunodeficiency Virus Type 1 p6Gag Protein Produces a Mutant That Fails To Package Its Envelope Protein. J. Virol. 73: 19-28 [Abstract] [Full Text]  
  • Selig, L., Pages, J.-C., Tanchou, V., Preveral, S., Berlioz-Torrent, C., Liu, L. X., Erdtmann, L., Darlix, J.-L., Benarous, R., Benichou, S. (1999). Interaction with the p6 Domain of the Gag Precursor Mediates Incorporation into Virions of Vpr and Vpx Proteins from Primate Lentiviruses. J. Virol. 73: 592-600 [Abstract] [Full Text]  
  • Jenkins, Y., McEntee, M., Weis, K., Greene, W. C. (1998). Characterization of HIV-1 Vpr Nuclear Import: Analysis of Signals and Pathways. JCB 143: 875-885 [Abstract] [Full Text]  
  • Kobinger, G. P., Borsetti, A., Nie, Z., Mercier, J., Daniel, N., Gottlinger, H. G., Cohen, E. A. (1998). Virion-Targeted Viral Inactivation of Human Immunodeficiency Virus Type 1 by Using Vpr Fusion Proteins. J. Virol. 72: 5441-5448 [Abstract] [Full Text]  
  • Fouchier, R. A. M., Meyer, B. E., Simon, J. H. M., Fischer, U., Albright, A. V., Gonzalez-Scarano, F., Malim, M. H. (1998). Interaction of the Human Immunodeficiency Virus Type 1 Vpr Protein with the Nuclear Pore Complex. J. Virol. 72: 6004-6013 [Abstract] [Full Text]  
  • Garnier, L., Ratner, L., Rovinski, B., Cao, S.-X., Wills, J. W. (1998). Particle Size Determinants in the Human Immunodeficiency Virus Type 1 Gag Protein. J. Virol. 72: 4667-4677 [Abstract] [Full Text]  
  • Pancio, H. A., Ratner, L. (1998). Human Immunodeficiency Virus Type 2 Vpx-Gag Interaction. J. Virol. 72: 5271-5275 [Abstract] [Full Text]  
  • Felzien, L. K., Woffendin, C., Hottiger, M. O., Subbramanian, R. A., Cohen, E. A., Nabel, G. J. (1998). HIV transcriptional activation by the accessory protein, VPR, is mediated by the p300 co-activator. Proc. Natl. Acad. Sci. USA 95: 5281-5286 [Abstract] [Full Text]  
  • BouHamdan, M., Xue, Y., Baudat, Y., Hu, B., Sire, J., Pomerantz, R. J., Duan, L.-X. (1998). Diversity of HIV-1 Vpr Interactions Involves Usage of the WXXF Motif of Host Cell Proteins. J. Biol. Chem. 273: 8009-8016 [Abstract] [Full Text]  
  • Stark, L. A., Hay, R. T. (1998). Human Immunodeficiency Virus Type 1 (HIV-1) Viral Protein R (Vpr) Interacts with Lys-tRNA Synthetase: Implications for Priming of HIV-1 Reverse Transcription. J. Virol. 72: 3037-3044 [Abstract] [Full Text]  
  • Yu, X.-F., Dawson, L., Tian, C.-J., Flexner, C., Dettenhofer, M. (1998). Mutations of the Human Immunodeficiency Virus Type 1 p6Gag Domain Result in Reduced Retention of Pol Proteins during Virus Assembly. J. Virol. 72: 3412-3417 [Abstract] [Full Text]  
  • de Rocquigny, H., Petitjean, P., Tanchou, V., Decimo, D., Drouot, L., Delaunay, T., Darlix, J.-L., Roques, B. P. (1997). The Zinc Fingers of HIV Nucleocapsid Protein NCp7 Direct Interactions with the Viral Regulatory Protein Vpr. J. Biol. Chem. 272: 30753-30759 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»