This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Muster, T. Articles by Palese, P. Search for Related Content PubMed PubMed Citation Articles by Muster, T. Articles by Palese, P.

 Previous Article  |  Next Article 

J. Virol., 11 1995, 6678-6686, Vol 69, No. 11
Copyright © 1995, American Society for Microbiology

Mucosal model of immunization against human immunodeficiency virus type 1 with a chimeric influenza virus

T Muster, B Ferko, A Klima, M Purtscher, A Trkola, P Schulz, A Grassauer, OG Engelhardt, A Garcia-Sastre and P Palese
Institut fur Angewandte Mikrobiologie, Universitat fur Bodenkultur, Vienna, Austria.

Previously, we constructed a chimeric influenza virus that expresses the highly conserved amino acid sequence ELDKWA of gp41 of human immunodeficiency virus type 1 (HIV-1). Antisera elicited in mice by infection with this chimeric virus showed neutralizing activity against distantly related HIV-1 isolates (T. Muster, R. Guinea, A. Trkola, M. Purtscher, A. Klima, F. Steindl, P. Palese, and H. Katinger, J. Virol. 68:4031-4034, 1994). In the present study, we demonstrated that intranasal immunizations with this chimeric virus are also able to induce a humoral immune response at the mucosal level. The immunized mice had ELDKWA-specific immunoglobulins A in respiratory, intestinal, and vaginal secretions. Sustained levels of these secretory immunoglobulins A were detectable for more than 1 year after immunization. The results show that influenza virus can be used to efficiently induce secretory antibodies against antigens from foreign pathogens. Since long-lasting mucosal immunity in the genital and intestinal tracts might be essential for protective immunity against HIV-1, influenza virus appears to be a promising vector for HIV-1- derived immunogens.

This article has been cited by other articles:

• Li, Z.-N., Mueller, S. N., Ye, L., Bu, Z., Yang, C., Ahmed, R., Steinhauer, D. A. (2005). Chimeric Influenza Virus Hemagglutinin Proteins Containing Large Domains of the Bacillus anthracis Protective Antigen: Protein Characterization, Incorporation into Infectious Influenza Viruses, and Antigenicity. J. Virol. 79: 10003-10012 [Abstract] [Full Text]  
• Binley, J. M., Wrin, T., Korber, B., Zwick, M. B., Wang, M., Chappey, C., Stiegler, G., Kunert, R., Zolla-Pazner, S., Katinger, H., Petropoulos, C. J., Burton, D. R. (2004). Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies. J. Virol. 78: 13232-13252 [Abstract] [Full Text]  
• Nakaya, Y., Nakaya, T., Park, M.-S., Cros, J., Imanishi, J., Palese, P., Garcia-Sastre, A. (2004). Induction of Cellular Immune Responses to Simian Immunodeficiency Virus Gag by Two Recombinant Negative-Strand RNA Virus Vectors. J. Virol. 78: 9366-9375 [Abstract] [Full Text]  
• Hatta, M., Goto, H., Kawaoka, Y. (2004). Influenza B Virus Requires BM2 Protein for Replication. J. Virol. 78: 5576-5583 [Abstract] [Full Text]  
• Schlehuber, L. D., Rose, J. K. (2004). Prediction and Identification of a Permissive Epitope Insertion Site in the Vesicular Stomatitis Virus Glycoprotein. J. Virol. 78: 5079-5087 [Abstract] [Full Text]  
• Shinya, K., Fujii, Y., Ito, H., Ito, T., Kawaoka, Y. (2004). Characterization of a Neuraminidase-Deficient Influenza A Virus as a Potential Gene Delivery Vector and a Live Vaccine. J. Virol. 78: 3083-3088 [Abstract] [Full Text]  
• Garulli, B., Kawaoka, Y., Castrucci, M. R. (2004). Mucosal and Systemic Immune Responses to a Human Immunodeficiency Virus Type 1 Epitope Induced upon Vaginal Infection with a Recombinant Influenza A Virus. J. Virol. 78: 1020-1025 [Abstract] [Full Text]  
• Fujii, Y., Goto, H., Watanabe, T., Yoshida, T., Kawaoka, Y. (2003). Selective incorporation of influenza virus RNA segments into virions. Proc. Natl. Acad. Sci. USA 100: 2002-2007 [Abstract] [Full Text]  
• Neumann, G., Whitt, M. A., Kawaoka, Y. (2002). A decade after the generation of a negative-sense RNA virus from cloned cDNA - what have we learned?. J. Gen. Virol. 83: 2635-2662 [Abstract] [Full Text]  
• Parks, G. D., Alexander-Miller, M. A. (2002). High avidity cytotoxic T lymphocytes to a foreign antigen are efficiently activated following immunization with a recombinant paramyxovirus, simian virus 5. J. Gen. Virol. 83: 1167-1172 [Abstract] [Full Text]  
• Ferko, B., Stasakova, J., Sereinig, S., Romanova, J., Katinger, D., Niebler, B., Katinger, H., Egorov, A. (2001). Hyperattenuated Recombinant Influenza A Virus Nonstructural-Protein-Encoding Vectors Induce Human Immunodeficiency Virus Type 1 Nef-Specific Systemic and Mucosal Immune Responses in Mice. J. Virol. 75: 8899-8908 [Abstract] [Full Text]  
• Zwick, M. B., Bonnycastle, L. L. C., Menendez, A., Irving, M. B., Barbas, C. F. III, Parren, P. W. H. I., Burton, D. R., Scott, J. K. (2001). Identification and Characterization of a Peptide That Specifically Binds the Human, Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody b12. J. Virol. 75: 6692-6699 [Abstract] [Full Text]  
• Moore, J. P., Parren, P. W. H. I., Burton, D. R. (2001). Genetic Subtypes, Humoral Immunity, and Human Immunodeficiency Virus Type 1 Vaccine Development. J. Virol. 75: 5721-5729 [Full Text]  
• Zheng, H., Palese, P., García-Sastre, A. (2000). Antitumor Properties of Influenza Virus Vectors. Cancer Res. 60: 6972-6976 [Abstract] [Full Text]  
• Dupuy, C., Buzoni-Gatel, D., Touze, A., Bout, D., Coursaget, P. (1999). Nasal Immunization of Mice with Human Papillomavirus Type 16 (HPV-16) Virus-Like Particles or with the HPV-16 L1 Gene Elicits Specific Cytotoxic T Lymphocytes in Vaginal Draining Lymph Nodes. J. Virol. 73: 9063-9071 [Abstract] [Full Text]  
• Palese, P. (1998). RNA virus vectors: Where are we and where do we need to go?. Proc. Natl. Acad. Sci. USA 95: 12750-12752 [Full Text]  
• Staczek, J., Gilleland, H. E. Jr., Gilleland, L. B., Harty, R. N., Garcia-Sastre, A., Engelhardt, O. G., Palese, P. (1998). A Chimeric Influenza Virus Expressing an Epitope of Outer Membrane Protein F of Pseudomonas aeruginosa Affords Protection against Challenge with P. aeruginosa in a Murine Model of Chronic Pulmonary Infection. Infect. Immun. 66: 3990-3994 [Abstract] [Full Text]  
• VanCott, T. C., Kaminski, R. W., Mascola, J. R., Kalyanaraman, V. S., Wassef, N. M., Alving, C. R., Ulrich, J.T., Lowell, G. H., Birx, D. L. (1998). HIV-1 Neutralizing Antibodies in the Genital and Respiratory Tracts of Mice Intranasally Immunized with Oligomeric gp160. J. Immunol. 160: 2000-2012 [Abstract] [Full Text]