This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, H.
Right arrow Articles by Anderson, W. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, H.
Right arrow Articles by Anderson, W. F.

 Previous Article  |  Next Article 

J. Virol., Oct 1995, 6557-6562, Vol 69, No. 10
Copyright © 1995, American Society for Microbiology

Binding kinetics of ecotropic (Moloney) murine leukemia retrovirus with NIH 3T3 cells

H Yu, N Soong and WF Anderson
Gene Therapy Laboratories, Norris Cancer Center, University of Southern California School of Medicine, Los Angeles 90033, USA.

A quantitative analysis of the binding kinetics of intact Moloney murine leukemia retrovirus (MoMuLV) particles with NIH 3T3 cells was performed with an immunofluorescence flow cytometry assay. The virus- cell binding equilibrium dissociation constant (KD), expressed in terms of virus particle concentration, was measured to be 8.5 (+/- 6.4) x 10(- 12) M at 4 degrees C and was three- to sixfold lower at temperatures above 15 degrees C. The KD of virus binding is about 1,000-fold lower than the KD of purified MoMuLV envelope. The association rate constant was determined to be 2.5 (+/- 0.9) x 10(9) M-1 min-1 at 4 degrees C and was 5- to 10-fold higher at temperatures above 15 degrees C. The apparent dissociation rate constant at 4 degrees C was 1.1 (+/- 0.4) x 10(-3) min-1 and was doubled for every 10 degrees C increase in temperature over the range tested (15 to 37 degrees C).


This article has been cited by other articles:

  • Burkhart, M. D., D'Agostino, P., Kayman, S. C., Pinter, A. (2005). Involvement of the C-Terminal Disulfide-Bonded Loop of Murine Leukemia Virus SU Protein in a Postbinding Step Critical for Viral Entry. J. Virol. 79: 7868-7876 [Abstract] [Full Text]  
  • Haim, H., Steiner, I., Panet, A. (2005). Synchronized Infection of Cell Cultures by Magnetically Controlled Virus. J. Virol. 79: 622-625 [Abstract] [Full Text]  
  • Qian, Z., Wang, H., Empig, C., Anderson, W. F., Albritton, L. M. (2004). Complementation of a Binding-Defective Retrovirus by a Host Cell Receptor Mutant. J. Virol. 78: 5766-5772 [Abstract] [Full Text]  
  • Kwon, Y. J., Hung, G., Anderson, W. F., Peng, C.-A., Yu, H. (2003). Determination of Infectious Retrovirus Concentration from Colony-Forming Assay with Quantitative Analysis. J. Virol. 77: 5712-5720 [Abstract] [Full Text]  
  • Burkhart, M. D., Kayman, S. C., He, Y., Pinter, A. (2003). Distinct Mechanisms of Neutralization by Monoclonal Antibodies Specific for Sites in the N-Terminal or C-Terminal Domain of Murine Leukemia Virus SU. J. Virol. 77: 3993-4003 [Abstract] [Full Text]  
  • Aguilar, H. C., Anderson, W. F., Cannon, P. M. (2002). Cytoplasmic Tail of Moloney Murine Leukemia Virus Envelope Protein Influences the Conformation of the Extracellular Domain: Implications for Mechanism of Action of the R Peptide. J. Virol. 77: 1281-1291 [Abstract] [Full Text]  
  • Lavillette, D., Boson, B., Russell, S. J., Cosset, F.-L. (2001). Activation of Membrane Fusion by Murine Leukemia Viruses Is Controlled in cis or in trans by Interactions between the Receptor-Binding Domain and a Conserved Disulfide Loop of the Carboxy Terminus of the Surface Glycoprotein. J. Virol. 75: 3685-3695 [Abstract] [Full Text]  
  • O'Doherty, U., Swiggard, W. J., Malim, M. H. (2000). Human Immunodeficiency Virus Type 1 Spinoculation Enhances Infection through Virus Binding. J. Virol. 74: 10074-10080 [Abstract] [Full Text]  
  • Bachrach, E., Marin, M., Pelegrin, M., Karavanas, G., Piechaczyk, M. (2000). Efficient Cell Infection by Moloney Murine Leukemia Virus-Derived Particles Requires Minimal Amounts of Envelope Glycoprotein. J. Virol. 74: 8480-8486 [Abstract] [Full Text]  
  • Chung, M., Kizhatil, K., Albritton, L. M., Gaulton, G. N. (1999). Induction of Syncytia by Neuropathogenic Murine Leukemia Viruses Depends on Receptor Density, Host Cell Determinants, and the Intrinsic Fusion Potential of Envelope Protein. J. Virol. 73: 9377-9385 [Abstract] [Full Text]  
  • Pizzato, M., Marlow, S. A., Blair, E. D., Takeuchi, Y. (1999). Initial Binding of Murine Leukemia Virus Particles to Cells Does Not Require Specific Env-Receptor Interaction. J. Virol. 73: 8599-8611 [Abstract] [Full Text]  
  • Zavorotinskaya, T., Albritton, L. M. (1999). Failure To Cleave Murine Leukemia Virus Envelope Protein Does Not Preclude Its Incorporation in Virions and Productive Virus-Receptor Interaction. J. Virol. 73: 5621-5629 [Abstract] [Full Text]  
  • Lee, S., Zhao, Y., Anderson, W. F. (1999). Receptor-Mediated Moloney Murine Leukemia Virus Entry Can Occur Independently of the Clathrin-Coated-Pit-Mediated Endocytic Pathway. J. Virol. 73: 5994-6005 [Abstract] [Full Text]  
  • Balliet, J. W., Berson, J., D'Cruz, C. M., Huang, J., Crane, J., Gilbert, J. M., Bates, P. (1999). Production and Characterization of a Soluble, Active Form of Tva, the Subgroup A Avian Sarcoma and Leukosis Virus Receptor. J. Virol. 73: 3054-3061 [Abstract] [Full Text]  
  • Zhao, Y., Zhu, L., Lee, S., Li, L., Chang, E., Soong, N.-W., Douer, D., Anderson, W. F. (1999). Identification of the block in targeted retroviral-mediated gene transfer. Proc. Natl. Acad. Sci. USA 96: 4005-4010 [Abstract] [Full Text]  
  • Kurre, P., Kiem, H.-P., Morris, J., Heyward, S., Battini, J.-L., Miller, A. D. (1999). Efficient Transduction by an Amphotropic Retrovirus Vector Is Dependent on High-Level Expression of the Cell Surface Virus Receptor. J. Virol. 73: 495-500 [Abstract] [Full Text]  
  • Han, J.-Y., Zhao, Y., Anderson, W. F., Cannon, P. M. (1998). Role of Variable Regions A and B in Receptor Binding Domain of Amphotropic Murine Leukemia Virus Envelope Protein. J. Virol. 72: 9101-9108 [Abstract] [Full Text]  
  • Zhao, Y., Zhu, L., Benedict, C. A., Chen, D., Anderson, W. F., Cannon, P. M. (1998). Functional Domains in the Retroviral Transmembrane Protein. J. Virol. 72: 5392-5398 [Abstract] [Full Text]  
  • Porter, C. D., Lukacs, K. V., Box, G., Takeuchi, Y., Collins, M. K. L. (1998). Cationic Liposomes Enhance the Rate of Transduction by a Recombinant Retroviral Vector In Vitro and In Vivo. J. Virol. 72: 4832-4840 [Abstract] [Full Text]  
  • Zhu, N.-L., Cannon, P. M., Chen, D., Anderson, W. F. (1998). Mutational Analysis of the Fusion Peptide of Moloney Murine Leukemia Virus Transmembrane Protein p15E. J. Virol. 72: 1632-1639 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»