The human immunodeficiency virus type 1 vpr gene arrests infected T cells in the G2 + M phase of the cell cycle

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J. Virol., Oct 1995, 6304-6313, Vol 69, No. 10
Copyright © 1995, American Society for Microbiology

The human immunodeficiency virus type 1 vpr gene arrests infected T cells in the G2 + M phase of the cell cycle

JB Jowett, V Planelles, B Poon, NP Shah, ML Chen and IS Chen
Department of Microbiology & Immunology, UCLA School of Medicine 90095- 1678, USA.

Human immunodeficiency virus type 1 (HIV-1) infection causes profound immunological defects in afflicted patients. Various mechanisms have been proposed to account for the immune dysfunction in AIDS ultimately leading to loss of CD4+ T cells, including HIV-1 envelope-mediated syncytium formation, apoptosis, and cytokine modulation. Here we present results which suggest a novel hypothesis for T-cell dysfunction. We show, using HIV-1 bearing a novel cell surface reporter gene, that infected cells are unable to progress normally through the cell cycle and became arrested in the G2 + M phase. Furthermore, we identify the HIV-1 vpr gene product as being both necessary and sufficient for eliciting this cell cycle arrest. Cell cycle arrest induced by Vpr correlates with an increase in the hyperphosphorylated (inactive) form of the cyclin-dependent serine/threonine kinase CDC2, consistent with an arrest of cells at the boundary of G2 and M.

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Mueller, S. M., Lang, S. M. (2002). The First HxRxG Motif in Simian Immunodeficiency Virus mac239 Vpr Is Crucial for G2/M Cell Cycle Arrest. J. Virol. 76: 11704-11709 [Abstract] [Full Text]
Nakahara, T., Nishimura, A., Tanaka, M., Ueno, T., Ishimoto, A., Sakai, H. (2002). Modulation of the Cell Division Cycle by Human Papillomavirus Type 18 E4. J. Virol. 76: 10914-10920 [Abstract] [Full Text]
Chen, R., Wang, H., Mansky, L. M. (2002). Roles of uracil-DNA glycosylase and dUTPase in virus replication. J. Gen. Virol. 83: 2339-2345 [Abstract] [Full Text]
Muthumani, K., Hwang, D. S., Desai, B. M., Zhang, D., Dayes, N., Green, D. R., Weiner, D. B. (2002). HIV-1 Vpr Induces Apoptosis through Caspase 9 in T Cells and Peripheral Blood Mononuclear Cells. J. Biol. Chem. 277: 37820-37831 [Abstract] [Full Text]
Kino, T., Gragerov, A., Slobodskaya, O., Tsopanomichalou, M., Chrousos, G. P., Pavlakis, G. N. (2002). Human Immunodeficiency Virus Type 1 (HIV-1) Accessory Protein Vpr Induces Transcription of the HIV-1 and Glucocorticoid-Responsive Promoters by Binding Directly to p300/CBP Coactivators. J. Virol. 76: 9724-9734 [Abstract] [Full Text]
Liang, M.-H., Geisbert, T., Yao, Y., Hinrichs, S. H., Giam, C.-Z. (2002). Human T-Lymphotropic Virus Type 1 Oncoprotein Tax Promotes S-Phase Entry but Blocks Mitosis. J. Virol. 76: 4022-4033 [Abstract] [Full Text]
Arganaraz, E., Cortes, M. J., Leibel, S., Lama, J. (2002). Human Immunodeficiency Virus Type 1 Vpr Protein Does Not Modulate Surface Expression of the CD4 Receptor. J. Virol. 76: 4125-4130 [Abstract] [Full Text]
Yang, O. O., Nguyen, P. T., Kalams, S. A., Dorfman, T., Gottlinger, H. G., Stewart, S., Chen, I. S. Y., Threlkeld, S., Walker, B. D. (2002). Nef-Mediated Resistance of Human Immunodeficiency Virus Type 1 to Antiviral Cytotoxic T Lymphocytes. J. Virol. 76: 1626-1631 [Abstract] [Full Text]
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Eckstein, D. A., Sherman, M. P., Penn, M. L., Chin, P. S., De Noronha, C. M.C., Greene, W. C., Goldsmith, M. A. (2001). HIV-1 Vpr Enhances Viral Burden by Facilitating Infection of Tissue Macrophages but Not Nondividing CD4+ T Cells. J. Exp. Med. 194: 1407-1419 [Abstract] [Full Text]
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Zhu, Y., Gelbard, H. A., Roshal, M., Pursell, S., Jamieson, B. D., Planelles, V. (2001). Comparison of Cell Cycle Arrest, Transactivation, and Apoptosis Induced by the Simian Immunodeficiency Virus SIVagm and Human Immunodeficiency Virus Type 1 vpr Genes. J. Virol. 75: 3791-3801 [Abstract] [Full Text]
Wang, X., Lewis, D. E. (2001). CD86 expression correlates with amounts of HIV produced by macrophages in vitro. J. Leukoc. Biol. 69: 405-413 [Abstract] [Full Text]
Sherman, M. P., de Noronha, C. M. C., Heusch, M. I., Greene, S., Greene, W. C. (2001). Nucleocytoplasmic Shuttling by Human Immunodeficiency Virus Type 1 Vpr. J. Virol. 75: 1522-1532 [Abstract] [Full Text]
Poggioli, G. J., Keefer, C., Connolly, J. L., Dermody, T. S., Tyler, K. L. (2000). Reovirus-Induced G2/M Cell Cycle Arrest Requires sigma 1s and Occurs in the Absence of Apoptosis. J. Virol. 74: 9562-9570 [Abstract] [Full Text]
Patel, C. A., Mukhtar, M., Pomerantz, R. J. (2000). Human Immunodeficiency Virus Type 1 Vpr Induces Apoptosis in Human Neuronal Cells. J. Virol. 74: 9717-9726 [Abstract] [Full Text]
Müller, B., Tessmer, U., Schubert, U., Kräusslich, H.-G. (2000). Human Immunodeficiency Virus Type 1 Vpr Protein Is Incorporated into the Virion in Significantly Smaller Amounts than Gag and Is Phosphorylated in Infected Cells. J. Virol. 74: 9727-9731 [Abstract] [Full Text]
Conti, L., Matarrese, P., Varano, B., Gauzzi, M. C., Sato, A., Malorni, W., Belardelli, F., Gessani, S. (2000). Dual Role of the HIV-1 Vpr Protein in the Modulation of the Apoptotic Response of T Cells. J. Immunol. 165: 3293-3300 [Abstract] [Full Text]
Muthumani, K., Kudchodkar, S., Papasavvas, E., Montaner, L. J., Weiner, D. B., Ayyavoo, V. (2000). HIV-1 Vpr regulates expression of {beta} chemokines in human primary lymphocytes and macrophages. J. Leukoc. Biol. 68: 366-372 [Abstract] [Full Text]
Sherman, M. P., de Noronha, C. M. C., Pearce, D., Greene, W. C. (2000). Human Immunodeficiency Virus Type 1 Vpr Contains Two Leucine-Rich Helices That Mediate Glucocorticoid Receptor Coactivation Independently of Its Effects on G2 Cell Cycle Arrest. J. Virol. 74: 8159-8165 [Abstract] [Full Text]
Mansky, L. M., Preveral, S., Selig, L., Benarous, R., Benichou, S. (2000). The Interaction of Vpr with Uracil DNA Glycosylase Modulates the Human Immunodeficiency Virus Type 1 In Vivo Mutation Rate. J. Virol. 74: 7039-7047 [Abstract] [Full Text]
Kamata, M., Aida, Y. (2000). Two Putative alpha -Helical Domains of Human Immunodeficiency Virus Type 1 Vpr Mediate Nuclear Localization by at Least Two Mechanisms. J. Virol. 74: 7179-7186 [Abstract] [Full Text]
Nishizawa, M., Kamata, M., Katsumata, R., Aida, Y. (2000). A Carboxy-Terminally Truncated Form of the Human Immunodeficiency Virus Type 1 Vpr Protein Induces Apoptosis via G1 Cell Cycle Arrest. J. Virol. 74: 6058-6067 [Abstract] [Full Text]
Accola, M. A., Öhagen, A., Göttlinger, H. G. (2000). Isolation of Human Immunodeficiency Virus Type 1 Cores: Retention of Vpr in the Absence of p6gag. J. Virol. 74: 6198-6202 [Abstract] [Full Text]
Kichler, A., Pages, J.-C., Leborgne, C., Druillennec, S., Lenoir, C., Coulaud, D., Delain, E., Le Cam, E., Roques, B. P., Danos, O. (2000). Efficient DNA Transfection Mediated by the C-Terminal Domain of Human Immunodeficiency Virus Type 1 Viral Protein R. J. Virol. 74: 5424-5431 [Abstract] [Full Text]
Roux, P., Alfieri, C., Hrimech, M., Cohen, E. A., Tanner, J. E. (2000). Activation of Transcription Factors NF-kappa B and NF-IL-6 by Human Immunodeficiency Virus Type 1 Protein R (Vpr) Induces Interleukin-8 Expression. J. Virol. 74: 4658-4665 [Abstract] [Full Text]
Sawaya, B. E., Khalili, K., Gordon, J., Srinivasan, A., Richardson, M., Rappaport, J., Amini, S. (2000). Transdominant Activity of Human Immunodeficiency Virus Type 1 Vpr with a Mutation at Residue R73. J. Virol. 74: 4877-4881 [Abstract] [Full Text]
Stone, D. M., Norton, L. K., Davis, W. C. (2000). Spontaneously proliferating lymphocytes from bovine leukaemia virus-infected, lymphocytotic cattle are not the virus-expressing lymphocytes, as these cells are delayed in G0/G1 of the cell cycle and are spared from apoptosis. J. Gen. Virol. 81: 971-981 [Abstract] [Full Text]
Stewart, S. A., Poon, B., Song, J. Y., Chen, I. S. Y. (2000). Human Immunodeficiency Virus Type 1 Vpr Induces Apoptosis through Caspase Activation. J. Virol. 74: 3105-3111 [Abstract] [Full Text]
Masuda, M., Nagai, Y., Oshima, N., Tanaka, K., Murakami, H., Igarashi, H., Okayama, H. (2000). Genetic Studies with the Fission Yeast Schizosaccharomyces pombe Suggest Involvement of Wee1, Ppa2, and Rad24 in Induction of Cell Cycle Arrest by Human Immunodeficiency Virus Type 1 Vpr. J. Virol. 74: 2636-2646 [Abstract] [Full Text]
Kashanchi, F., Agbottah, E. T., Pise-Masison, C. A., Mahieux, R., Duvall, J., Kumar, A., Brady, J. N. (2000). Cell Cycle-Regulated Transcription by the Human Immunodeficiency Virus Type 1 Tat Transactivator. J. Virol. 74: 652-660 [Abstract] [Full Text]
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Accola, M. A., Bukovsky, A. A., Jones, M. S., Göttlinger, H. G. (1999). A Conserved Dileucine-Containing Motif in p6gag Governs the Particle Association of Vpx and Vpr of Simian Immunodeficiency Viruses SIVmac and SIVagm. J. Virol. 73: 9992-9999 [Abstract] [Full Text]
Stewart, S. A., Poon, B., Jowett, J. B. M., Xie, Y., Chen, I. S. Y. (1999). Lentiviral delivery of HIV-1 Vpr protein induces apoptosis in transformed cells. Proc. Natl. Acad. Sci. USA 96: 12039-12043 [Abstract] [Full Text]
Jowett, J. B., Xie, Y.-m., Chen, I. S. Y. (1999). The Presence of Human Immunodeficiency Virus Type 1 Vpr Correlates with a Decrease in the Frequency of Mutations in a Plasmid Shuttle Vector. J. Virol. 73: 7132-7137 [Abstract] [Full Text]
Shtrichman, R., Sharf, R., Barr, H., Dobner, T., Kleinberger, T. (1999). Induction of apoptosis by adenovirus E4orf4 protein is specific to transformed cells and requires an interaction with protein phosphatase 2A. Proc. Natl. Acad. Sci. USA 96: 10080-10085 [Abstract] [Full Text]
Gummuluru, S., Emerman, M. (1999). Cell Cycle- and Vpr-Mediated Regulation of Human Immunodeficiency Virus Type 1�Expression in Primary and Transformed T-Cell Lines. J. Virol. 73: 5422-5430 [Abstract] [Full Text]
Kovalev, G., Duus, K., Wang, L., Lee, R., Bonyhadi, M., Ho, D., McCune, J. M., Kaneshima, H., Su, L. (1999). Induction of MHC Class I Expression on Immature Thymocytes in HIV-1-Infected SCID-hu Thy/Liv Mice: Evidence of Indirect Mechanisms. J. Immunol. 162: 7555-7562 [Abstract] [Full Text]
Fuller, J. F., McAdara, J., Yaron, Y., Sakaguchi, M., Fraser, J. K., Gasson, J. C. (1999). Characterization of HOX Gene Expression During Myelopoiesis: Role of HOX A5 in Lineage Commitment and Maturation. Blood 93: 3391-3400 [Abstract] [Full Text]
Hrimech, M., Yao, X.-J., Bachand, F., Rougeau, N., Cohen, E. A. (1999). Human Immunodeficiency Virus Type 1�(HIV-1) Vpr Functions as an Immediate-Early Protein during HIV-1 Infection. J. Virol. 73: 4101-4109 [Abstract] [Full Text]
Piller, S. C., Ewart, G. D., Jans, D. A., Gage, P. W., Cox, G. B. (1999). The Amino-Terminal Region of Vpr from Human Immunodeficiency Virus Type 1�Forms Ion Channels and Kills Neurons. J. Virol. 73: 4230-4238 [Abstract] [Full Text]
White, S. M., Renda, M., Nam, N.-Y., Klimatcheva, E., Zhu, Y., Fisk, J., Halterman, M., Rimel, B. J., Federoff, H., Pandya, S., Rosenblatt, J. D., Planelles, V. (1999). Lentivirus Vectors Using Human and Simian Immunodeficiency Virus Elements. J. Virol. 73: 2832-2840 [Abstract] [Full Text]
Chen, M., Elder, R. T., Yu, M., O'Gorman, M. G., Selig, L., Benarous, R., Yamamoto, A., Zhao, Y. (1999). Mutational Analysis of Vpr-Induced G2 Arrest, Nuclear Localization, and Cell Death in Fission Yeast. J. Virol. 73: 3236-3245 [Abstract] [Full Text]
Bachand, F., Yao, X.-J., Hrimech, M., Rougeau, N., Cohen, E. A. (1999). Incorporation of Vpr into Human Immunodeficiency Virus Type 1�Requires a Direct Interaction with the p6 Domain of the p55 Gag Precursor. J. Biol. Chem. 274: 9083-9091 [Abstract] [Full Text]
Gasmi, M., Glynn, J., Jin, M.-J., Jolly, D. J., Yee, J.-K., Chen, S.-T. (1999). Requirements for Efficient Production and Transduction of Human Immunodeficiency Virus Type 1-Based Vectors. J. Virol. 73: 1828-1834 [Abstract] [Full Text]
Willetts, K. E., Rey, F., Agostini, I., Navarro, J.-M., Baudat, Y., Vigne, R., Sire, J. (1999). DNA Repair Enzyme Uracil DNA Glycosylase Is Specifically Incorporated into Human Immunodeficiency Virus Type 1�Viral Particles through a Vpr-Independent Mechanism. J. Virol. 73: 1682-1688 [Abstract] [Full Text]
Kino, T., Gragerov, A., Kopp, J. B., Stauber, R. H., Pavlakis, G. N., Chrousos, G. P. (1999). The HIV-1 Virion-associated Protein Vpr Is a Coactivator of the Human Glucocorticoid Receptor. J. Exp. Med. 189: 51-62 [Abstract] [Full Text]
Selig, L., Pages, J.-C., Tanchou, V., Preveral, S., Berlioz-Torrent, C., Liu, L. X., Erdtmann, L., Darlix, J.-L., Benarous, R., Benichou, S. (1999). Interaction with the p6 Domain of the Gag Precursor Mediates Incorporation into Virions of Vpr and Vpx Proteins from Primate Lentiviruses. J. Virol. 73: 592-600 [Abstract] [Full Text]
Jenkins, Y., McEntee, M., Weis, K., Greene, W. C. (1998). Characterization of HIV-1 Vpr Nuclear Import: Analysis of Signals and Pathways. J. Cell Biol. 143: 875-885 [Abstract] [Full Text]
Kitchen, S. G., Korin, Y. D., Roth, M. D., Landay, A., Zack, J. A. (1998). Costimulation of Naive CD8+ Lymphocytes Induces CD4 Expression and Allows Human Immunodeficiency Virus Type 1�Infection. J. Virol. 72: 9054-9060 [Abstract] [Full Text]
al-Ramadi, B. K., Zhang, H., Bothwell, A. L.�M. (1998). Cell-cycle arrest and apoptosis hypersusceptibility as a consequence of Lck deficiency in nontransformed T lymphocytes. Proc. Natl. Acad. Sci. USA 95: 12498-12503 [Abstract] [Full Text]
Gozlan, J., Lathey, J. L., Spector, S. A. (1998). Human Immunodeficiency Virus Type 1�Induction Mediated by Genistein Is Linked to Cell Cycle Arrest in G2. J. Virol. 72: 8174-8180 [Abstract] [Full Text]

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