This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stamatatos, L. Articles by Cheng-Mayer, C. Search for Related Content PubMed PubMed Citation Articles by Stamatatos, L. Articles by Cheng-Mayer, C.

 Previous Article  |  Next Article 

J. Virol., Oct 1995, 6191-6198, Vol 69, No. 10
Copyright © 1995, American Society for Microbiology

Structural modulations of the envelope gp120 glycoprotein of human immunodeficiency virus type 1 upon oligomerization and differential V3 loop epitope exposure of isolates displaying distinct tropism upon virion-soluble receptor binding

L Stamatatos and C Cheng-Mayer
Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016, USA.

We investigated the binding of conformation-dependent anti-V2, anti-V3, and anti-CD4-binding site monoclonal antibodies to monomeric and virion- associated gp120 from human immunodeficiency virus type 1 isolates displaying marked differences in cell tropism. For all viruses examined, we found that the half-maximal binding values of the anti-V2 and anti-CD4-binding site antibodies with virion-associated gp120 were higher than those with monomeric gp120, but the maximum amount of antibodies bound was diminished only for one of the anti-V2 antibodies tested. These observations suggest that upon gp120 oligomerization, the V2 loop and CD4-binding site undergo conformational changes and that particular epitopes within these domains are occluded in the oligomeric gp120. In contrast, although the overall binding patterns and half- maximal binding values of the anti-V3 loop antibodies tested were similar with monomeric and oligomeric gp120, all the V3 loop epitopes examined were less accessible to antibody binding on the virion surface. This masking of the V3 loop is more pronounced for the primary- like macrophage-tropic isolates examined. Lastly, we observe that upon soluble receptor-virion binding, specific V3 loop epitopes that differ for viruses displaying different tropisms are exposed.

This article has been cited by other articles:

• Ching, L. K., Vlachogiannis, G., Bosch, K. A., Stamatatos, L. (2008). The First Hypervariable Region of the gp120 Env Glycoprotein Defines the Neutralizing Susceptibility of Heterologous Human Immunodeficiency Virus Type 1 Isolates to Neutralizing Antibodies Elicited by the SF162gp140 Immunogen. J. Virol. 82: 949-956 [Abstract] [Full Text]  
• Yuan, W., Bazick, J., Sodroski, J. (2006). Characterization of the Multiple Conformational States of Free Monomeric and Trimeric Human Immunodeficiency Virus Envelope Glycoproteins after Fixation by Cross-Linker. J. Virol. 80: 6725-6737 [Abstract] [Full Text]  
• Watabe, T., Kishino, H., Okuhara, Y., Kitazoe, Y. (2006). Fold Recognition of the Human Immunodeficiency Virus Type 1 V3 Loop and Flexibility of Its Crown Structure During the Course of Adaptation to a Host. Genetics 172: 1385-1396 [Abstract] [Full Text]  
• Poon, B., Hsu, J. F., Gudeman, V., Chen, I. S. Y., Grovit-Ferbas, K. (2005). Formaldehyde-Treated, Heat-Inactivated Virions with Increased Human Immunodeficiency Virus Type 1 Env Can Be Used To Induce High-Titer Neutralizing Antibody Responses. J. Virol. 79: 10210-10217 [Abstract] [Full Text]  
• Lusso, P., Earl, P. L., Sironi, F., Santoro, F., Ripamonti, C., Scarlatti, G., Longhi, R., Berger, E. A., Burastero, S. E. (2005). Cryptic Nature of a Conserved, CD4-Inducible V3 Loop Neutralization Epitope in the Native Envelope Glycoprotein Oligomer of CCR5-Restricted, but Not CXCR4-Using, Primary Human Immunodeficiency Virus Type 1 Strains. J. Virol. 79: 6957-6968 [Abstract] [Full Text]  
• Xiang, S.-H., Farzan, M., Si, Z., Madani, N., Wang, L., Rosenberg, E., Robinson, J., Sodroski, J. (2005). Functional Mimicry of a Human Immunodeficiency Virus Type 1 Coreceptor by a Neutralizing Monoclonal Antibody. J. Virol. 79: 6068-6077 [Abstract] [Full Text]  
• Poon, B., Safrit, J. T., McClure, H., Kitchen, C., Hsu, J. F., Gudeman, V., Petropoulos, C., Wrin, T., Chen, I. S. Y., Grovit-Ferbas, K. (2005). Induction of Humoral Immune Responses following Vaccination with Envelope-Containing, Formaldehyde-Treated, Thermally Inactivated Human Immunodeficiency Virus Type 1. J. Virol. 79: 4927-4935 [Abstract] [Full Text]  
• McCaffrey, R. A., Saunders, C., Hensel, M., Stamatatos, L. (2004). N-Linked Glycosylation of the V3 Loop and the Immunologically Silent Face of gp120 Protects Human Immunodeficiency Virus Type 1 SF162 from Neutralization by Anti-gp120 and Anti-gp41 Antibodies. J. Virol. 78: 3279-3295 [Abstract] [Full Text]  
• Gorny, M. K., Revesz, K., Williams, C., Volsky, B., Louder, M. K., Anyangwe, C. A., Krachmarov, C., Kayman, S. C., Pinter, A., Nadas, A., Nyambi, P. N., Mascola, J. R., Zolla-Pazner, S. (2004). The V3 Loop Is Accessible on the Surface of Most Human Immunodeficiency Virus Type 1 Primary Isolates and Serves as a Neutralization Epitope. J. Virol. 78: 2394-2404 [Abstract] [Full Text]  
• Srivastava, I. K., Stamatatos, L., Kan, E., Vajdy, M., Lian, Y., Hilt, S., Martin, L., Vita, C., Zhu, P., Roux, K. H., Vojtech, L., C. Montefiori, D., Donnelly, J., Ulmer, J. B., Barnett, S. W. (2003). Purification, Characterization, and Immunogenicity of a Soluble Trimeric Envelope Protein Containing a Partial Deletion of the V2 Loop Derived from SF162, an R5-Tropic Human Immunodeficiency Virus Type 1 Isolate. J. Virol. 77: 11244-11259 [Abstract] [Full Text]  
• Zwick, M. B., Kelleher, R., Jensen, R., Labrijn, A. F., Wang, M., Quinnan, G. V. Jr., Parren, P. W. H. I., Burton, D. R. (2003). A Novel Human Antibody against Human Immunodeficiency Virus Type 1 gp120 Is V1, V2, and V3 Loop Dependent and Helps Delimit the Epitope of the Broadly Neutralizing Antibody Immunoglobulin G1 b12. J. Virol. 77: 6965-6978 [Abstract] [Full Text]  
• Srivastava, I. K., Stamatatos, L., Legg, H., Kan, E., Fong, A., Coates, S. R., Leung, L., Wininger, M., Donnelly, J. J., Ulmer, J. B., Barnett, S. W. (2002). Purification and Characterization of Oligomeric Envelope Glycoprotein from a Primary R5 Subtype B Human Immunodeficiency Virus. J. Virol. 76: 2835-2847 [Abstract] [Full Text]  
• Chakrabarti, L. A., Ivanovic, T., Cheng-Mayer, C. (2002). Properties of the Surface Envelope Glycoprotein Associated with Virulence of Simian-Human Immunodeficiency Virus SHIVSF33A Molecular Clones. J. Virol. 76: 1588-1599 [Abstract] [Full Text]  
• Papandreou, M.-J., Barbouche, R., Guieu, R., Kieny, M. P., Fenouillet, E. (2002). The alpha -Glucosidase Inhibitor 1-Deoxynojirimycin Blocks Human Immunodeficiency Virus Envelope Glycoprotein-Mediated Membrane Fusion at the CXCR4 Binding Step. Mol. Pharmacol. 61: 186-193 [Abstract] [Full Text]  
• Zhu, C.-B., Zhu, L., Holz-Smith, S., Matthews, T. J., Chen, C. H. (2001). The role of the third beta strand in gp120 conformation and neutralization sensitivity of the HIV-1 primary isolate DH012. Proc. Natl. Acad. Sci. USA 10.1073/pnas.261359098v1 [Abstract] [Full Text]  
• Mbah, H. A., Burda, S., Gorny, M. K., Williams, C., Revesz, K., Zolla-Pazner, S., Nyambi, P. N. (2001). Effect of Soluble CD4 on Exposure of Epitopes on Primary, Intact, Native Human Immunodeficiency Virus Type 1 Virions of Different Genetic Clades. J. Virol. 75: 7785-7788 [Abstract] [Full Text]  
• Barnett, S. W., Lu, S., Srivastava, I., Cherpelis, S., Gettie, A., Blanchard, J., Wang, S., Mboudjeka, I., Leung, L., Lian, Y., Fong, A., Buckner, C., Ly, A., Hilt, S., Ulmer, J., Wild, C. T., Mascola, J. R., Stamatatos, L. (2001). The Ability of an Oligomeric Human Immunodeficiency Virus Type 1 (HIV-1) Envelope Antigen To Elicit Neutralizing Antibodies against Primary HIV-1 Isolates Is Improved following Partial Deletion of the Second Hypervariable Region. J. Virol. 75: 5526-5540 [Abstract] [Full Text]  
• York, J., Follis, K. E., Trahey, M., Nyambi, P. N., Zolla-Pazner, S., Nunberg, J. H. (2001). Antibody Binding and Neutralization of Primary and T-Cell Line-Adapted Isolates of Human Immunodeficiency Virus Type 1. J. Virol. 75: 2741-2752 [Abstract] [Full Text]  
• Cherpelis, S., Shrivastava, I., Gettie, A., Jin, X., Ho, D. D., Barnett, S. W., Stamatatos, L. (2001). DNA Vaccination with the Human Immunodeficiency Virus Type 1 SF162{Delta}V2 Envelope Elicits Immune Responses That Offer Partial Protection from Simian/Human Immunodeficiency Virus Infection to CD8+ T-Cell-Depleted Rhesus Macaques. J. Virol. 75: 1547-1550 [Abstract] [Full Text]  
• Ly, A., Stamatatos, L. (2000). V2 Loop Glycosylation of the Human Immunodeficiency Virus Type 1 SF162 Envelope Facilitates Interaction of This Protein with CD4 and CCR5 Receptors and Protects the Virus from Neutralization by Anti-V3 Loop and Anti-CD4 Binding Site Antibodies. J. Virol. 74: 6769-6776 [Abstract] [Full Text]  
• Nyambi, P. N., Mbah, H. A., Burda, S., Williams, C., Gorny, M. K., Nádas, A., Zolla-Pazner, S. (2000). Conserved and Exposed Epitopes on Intact, Native, Primary Human Immunodeficiency Virus Type 1 Virions of Group M. J. Virol. 74: 7096-7107 [Abstract] [Full Text]  
• Grovit-Ferbas, K., Hsu, J. F., Ferbas, J., Gudeman, V., Chen, I. S. Y. (2000). Enhanced Binding of Antibodies to Neutralization Epitopes following Thermal and Chemical Inactivation of Human Immunodeficiency Virus Type 1. J. Virol. 74: 5802-5809 [Abstract] [Full Text]  
• Center, R. J., Earl, P. L., Lebowitz, J., Schuck, P., Moss, B. (2000). The Human Immunodeficiency Virus Type 1 gp120 V2 Domain Mediates gp41-Independent Intersubunit Contacts. J. Virol. 74: 4448-4455 [Abstract] [Full Text]  
• Merat, R., Raoul, H., Leste-Lasserre, T., Sonigo, P., Pancino, G. (1999). Variable Constraints on the Principal Immunodominant Domain of the Transmembrane Glycoprotein of Human Immunodeficiency Virus Type 1. J. Virol. 73: 5698-5706 [Abstract] [Full Text]  
• VanCott, T. C., Mascola, J. R., Loomis-Price, L. D., Sinangil, F., Zitomersky, N., McNeil, J., Robb, M. L., Birx, D. L., Barnett, S. (1999). Cross-Subtype Neutralizing Antibodies Induced in Baboons by a Subtype E gp120 Immunogen Based on an R5 Primary Human Immunodeficiency Virus Type 1 Envelope. J. Virol. 73: 4640-4650 [Abstract] [Full Text]  
• Beddows, S., Lister, S., Cheingsong, R., Bruck, C., Weber, J. (1999). Comparison of the Antibody Repertoire Generated in Healthy Volunteers following Immunization with a Monomeric Recombinant gp120 Construct Derived from a CCR5/CXCR4-Using Human Immunodeficiency Virus Type 1 Isolate with Sera from Naturally Infected Individuals. J. Virol. 73: 1740-1745 [Abstract] [Full Text]  
• Nyambi, P. N., Gorny, M. K., Bastiani, L., van der Groen, G., Williams, C., Zolla-Pazner, S. (1998). Mapping of Epitopes Exposed on Intact Human Immunodeficiency Virus Type 1 (HIV-1) Virions: a New Strategy for Studying the Immunologic Relatedness of HIV-1. J. Virol. 72: 9384-9391 [Abstract] [Full Text]  
• Stamatatos, L., Cheng-Mayer, C. (1998). An Envelope Modification That Renders a Primary, Neutralization-Resistant Clade B Human Immunodeficiency Virus Type 1 Isolate Highly Susceptible to Neutralization by Sera from Other Clades. J. Virol. 72: 7840-7845 [Abstract] [Full Text]  
• Opstelten, D.-J. E., Wallin, M., Garoff, H. (1998). Moloney Murine Leukemia Virus Envelope Protein Subunits, gp70 and Pr15E, Form a Stable Disulfide-Linked Complex. J. Virol. 72: 6537-6545 [Abstract] [Full Text]  
• Trkola, A., Ketas, T., KewalRamani, V. N., Endorf, F., Binley, J. M., Katinger, H., Robinson, J., Littman, D. R., Moore, J. P. (1998). Neutralization Sensitivity of Human Immunodeficiency Virus Type 1 Primary Isolates to Antibodies and CD4-Based Reagents Is Independent of Coreceptor Usage. J. Virol. 72: 1876-1885 [Abstract] [Full Text]  
• Poon, B., Chen, I. S. Y. (1998). Identification of a Domain within the Human T-Cell Leukemia Virus Type 2 Envelope Required for Syncytium Induction and Replication. J. Virol. 72: 1959-1966 [Abstract] [Full Text]  
• Richardson, J., Moraillon, A., Crespeau, F., Baud, S., Sonigo, P., Pancino, G. (1998). Delayed Infection after Immunization with a Peptide from the Transmembrane Glycoprotein of the Feline Immunodeficiency Virus. J. Virol. 72: 2406-2415 [Abstract] [Full Text]  
• McInerney, T. L., El Ahmar, W., Kemp, B. E., Poumbourios, P. (1998). Mutation-Directed Chemical Cross-Linking of Human Immunodeficiency Virus Type 1 gp41 Oligomers. J. Virol. 72: 1523-1533 [Abstract] [Full Text]  
• Hernandez, L. D., Peters, R. J., Delos, S. E., Young, J. A.T., Agard, D. A., White, J. M. (1997). Activation of a Retroviral Membrane Fusion Protein: Soluble Receptor-induced Liposome Binding of the ALSV Envelope Glycoprotein. J. Cell Biol. 139: 1455-1464 [Abstract] [Full Text]  
• Zhu, C.-B., Zhu, L., Holz-Smith, S., Matthews, T. J., Chen, C. H. (2001). The role of the third beta strand in gp120 conformation and neutralization sensitivity of the HIV-1 primary isolate DH012. Proc. Natl. Acad. Sci. USA 98: 15227-15232 [Abstract] [Full Text]