J. Virol., Oct 1995, 5929-5934, Vol 69, No. 10
R Schirmbeck, W Bohm, K Ando, FV Chisari and J Reimann
The efficiency of different vaccination techniques to prime in vivo major
histocompatibility complex class I-restricted murine cytotoxic T-
lymphocyte (CTL) precursors to hepatitis B virus small surface antigen
(HBsAg) was investigated. Mice were immunized either by injection of a low
dose of recombinant HBsAg protein preparations (native HBsAg particles or
denatured HBsAg monomers) without adjuvants, by infection with recombinant
vaccinia virus carrying an HBsAg-encoding gene, or by intramuscular
transfer of plasmid DNA encoding HBsAg under appropriate promoter control.
In H-2d mice, an Ld-restricted, S28-39-specific CTL response was
efficiently primed by all alternative vaccination techniques tested, but
the most potent priming of class I-restricted CTL to HBsAg in vivo was
observed with DNA immunization. Priming of anti-HBsAg CTL in H-2b mice was
not detectable after infection with a recombinant vaccinia virus or after
injection with exogenous recombinant HBsAg preparations. After DNA
immunization, however, both Kb- and Db-restricted CTL reactivity to HBsAg
emerged in H-2b mice. Hence, nucleic acid immunization revealed class
I-restricted CTL responsiveness to HBsAg in a mouse strain previously
considered to be a nonresponder at the CTL level. These results demonstrate
that the simple technique of nucleic acid immunization not only is
extremely efficient but also reveals an extended spectrum of potentially
immunogenic epitopes of protein antigens.
Copyright © 1995, American Society for Microbiology
Nucleic acid vaccination primes hepatitis B virus surface antigen- specific cytotoxic T lymphocytes in nonresponder mice
Institute of Medical Microbiology, University of Ulm, Germany.
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