This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bridge, E.
Right arrow Articles by Pettersson, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bridge, E.
Right arrow Articles by Pettersson, U.

 Previous Article  |  Next Article 

J. Virol., Jan 1995, 281-290, Vol 69, No. 1
Copyright © 1995, American Society for Microbiology

Dynamic organization of splicing factors in adenovirus-infected cells

E Bridge, DX Xia, M Carmo-Fonseca, B Cardinali, AI Lamond and U Pettersson
Department of Medical Genetics, Uppsala University, Sweden.

Adenovirus infection affects the nuclear distribution of host splicing factors. Late phase-infected cells contain discrete clusters of small nuclear ribonucleoproteins (snRNPs) that are separate from centers containing the viral 72-kilodalton DNA-binding protein (72K protein). In the present study, we demonstrate that these snRNP clusters also contain splicing factors from the SR protein family. We show that a previously described monoclonal antibody, 3C5, detects SR proteins. Furthermore, we demonstrate that late region 3 transcription occurs at a maximal rate in infected cultures in which greater than 90% of the cells contain the snRNP clusters, indicating that such cells are actively transcribing their late genes. During the onset of the late phase, the intranuclear distribution of splicing factors is very different from that seen after the late phase is established. When late viral transcription commences, cells with snRNP clusters are less prevalent than in cultures that are maintaining maximum levels of late transcription. Instead, a cell type which shows snRNPs, concentrated in foci that also contain the viral 72K DNA-binding protein is detected. This cell type disappears from cultures by 18 to 20 h after a high- multiplicity infection. These results suggest a dynamic organization of splicing factors in infected cells that can be correlated to the status of viral gene expression. Our work also provides an explanation for the differing results that have been published concerning the organization of splicing factors in the adenovirus-infected cell nucleus (L. F. Jimenez-Garcia and D. L. Spector, Cell 73:47-59, 1993). During the present study we observed that a monoclonal antibody against the SC-35 protein, which was used by Jimenez-Garcia and Spector to study the localization of the SC-35 splicing factor in adenovirus-infected cells, cross-reacts with the adenovirus 72K DNA-binding protein and is thus unsuitable for this type of study.


This article has been cited by other articles:

  • Miron, M.-J., Blanchette, P., Groitl, P., Dallaire, F., Teodoro, J. G., Li, S., Dobner, T., Branton, P. E. (2009). Localization and Importance of the Adenovirus E4orf4 Protein during Lytic Infection. J. Virol. 83: 1689-1699 [Abstract] [Full Text]  
  • Marshall, L. J., Moore, A. C., Ohki, M., Kitabayashi, I., Patterson, D., Ornelles, D. A. (2008). RUNX1 Permits E4orf6-Directed Nuclear Localization of the Adenovirus E1B-55K Protein and Associates with Centers of Viral DNA and RNA Synthesis. J. Virol. 82: 6395-6408 [Abstract] [Full Text]  
  • Gonzalez, R., Huang, W., Finnen, R., Bragg, C., Flint, S. J. (2006). Adenovirus E1B 55-Kilodalton Protein Is Required for both Regulation of mRNA Export and Efficient Entry into the Late Phase of Infection in Normal Human Fibroblasts. J. Virol. 80: 964-974 [Abstract] [Full Text]  
  • CUSTODIO, N., CARVALHO, C., CONDADO, I., ANTONIOU, M., BLENCOWE, B. J., CARMO-FONSECA, M. (2004). In vivo recruitment of exon junction complex proteins to transcription sites in mammalian cell nuclei. RNA 10: 622-633 [Abstract] [Full Text]  
  • Lindberg, A., Gama-Carvalho, M., Carmo-Fonseca, M., Kreivi, J.-P. (2004). A single RNA recognition motif in splicing factor ASF/SF2 directs it to nuclear sites of adenovirus transcription. J. Gen. Virol. 85: 603-608 [Abstract] [Full Text]  
  • Lee, T. W. R., Lawrence, F. J., Dauksaite, V., Akusjarvi, G., Blair, G. E., Matthews, D. A. (2004). Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins. J. Gen. Virol. 85: 185-196 [Abstract] [Full Text]  
  • Zhu, Y.-Q., Lu, Y., Tan, X.-D. (2003). Monochloramine induces reorganization of nuclear speckles and phosphorylation of SRp30 in human colonic epithelial cells: role of protein kinase C. Am. J. Physiol. Cell Physiol. 285: C1294-C1303 [Abstract] [Full Text]  
  • Tang, Z., Kuo, T., Shen, J., Lin, R.-J. (2000). Biochemical and Genetic Conservation of Fission Yeast Dsk1 and Human SR Protein-Specific Kinase 1. Mol. Cell. Biol. 20: 816-824 [Abstract] [Full Text]  
  • Jolly, C., Vourc'h, C., Robert-Nicoud, M., Morimoto, R. I. (1999). Intron-independent Association of Splicing Factors with Active Genes. JCB 145: 1133-1143 [Abstract] [Full Text]  
  • Lai, M.-C., Teh, B. H., Tarn, W.-Y. (1999). A Human Papillomavirus E2 Transcriptional Activator. THE INTERACTIONS WITH CELLULAR SPLICING FACTORS AND POTENTIAL FUNCTION IN PRE-mRNA PROCESSING. J. Biol. Chem. 274: 11832-11841 [Abstract] [Full Text]  
  • You, L.-R., Chen, C.-M., Yeh, T.-S., Tsai, T.-Y., Mai, R.-T., Lin, C.-H., Lee, Y.-H. W. (1999). Hepatitis C Virus Core Protein Interacts with Cellular Putative RNA Helicase. J. Virol. 73: 2841-2853 [Abstract] [Full Text]  
  • Angeletti, P. C., Engler, J. A. (1998). Adenovirus Preterminal Protein Binds to the CAD Enzyme at Active Sites of Viral DNA Replication on the Nuclear Matrix. J. Virol. 72: 2896-2904 [Abstract] [Full Text]  
  • Huang, W., Flint, S. J. (1998). The Tripartite Leader Sequence of Subgroup C Adenovirus Major Late mRNAs Can Increase the Efficiency of mRNA Export. J. Virol. 72: 225-235 [Abstract] [Full Text]  
  • Gama-Carvalho, M., Krauss, R. D., Chiang, L., Valcarcel, J., Green, M. R., Carmo-Fonseca, M. (1997). Targeting of U2AF65 to Sites of Active Splicing in the Nucleus. JCB 137: 975-987 [Abstract] [Full Text]  
  • Neugebauer, K M, Roth, M B (1997). Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription.. Genes Dev. 11: 1148-1159 [Abstract]  
  • Dirks, R., de Pauw, E., Raap, A. (1997). Splicing factors associate with nuclear HCMV-IE transcripts after transcriptional activation of the gene, but dissociate upon transcription inhibition: evidence for a dynamic organization of splicing factors. J. Cell Sci. 110: 515-522 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»