This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stewart, A. R. Articles by Wold, W. S. Search for Related Content PubMed PubMed Citation Articles by Stewart, A. R. Articles by Wold, W. S.

 Previous Article  |  Next Article 

J. Virol., Jan 1995, 172-181, Vol 69, No. 1
Copyright © 1995, American Society for Microbiology

The adenovirus E3 10.4K and 14.5K proteins, which function to prevent cytolysis by tumor necrosis factor and to down-regulate the epidermal growth factor receptor, are localized in the plasma membrane

AR Stewart, AE Tollefson, P Krajcsi, SP Yei and WS Wold
Department of Molecular Microbiology and Immunology, St. Louis University School of Medicine, Missouri 63104.

The adenovirus type 2 and 5 E3 10,400- and 14,500-molecular-weight (10.4K and 14.5K) proteins are both required to protect some cell lines from lysis by tumor necrosis factor and to down-regulate the epidermal growth factor receptor. We have shown previously that both 10.4K and 14.5K are integral membrane proteins and that 14.5K is phosphorylated and O glycosylated. The 10.4K protein coimmunoprecipitates with 14.5K, indicating that the two proteins function as a complex. Here we show, using immunofluorescence and two different cell surface-labeling techniques, that both proteins are localized in the plasma membrane. In addition, we show that trafficking of each protein to the plasma membrane depends on concomitant expression of the other protein. Finally, neither protein could be immunoprecipitated from conditioned media, indicating that neither is secreted. Taken together, these results suggest that the plasma membrane is the site at which 10.4K and 14.5K function to inhibit cytolysis by tumor necrosis factor and to down-regulate the epidermal growth factor receptor.

This article has been cited by other articles:

• Shah, A. H., Cianciola, N. L., Mills, J. L., Sonnichsen, F. D., Carlin, C. (2007). Adenovirus RID{alpha} regulates endosome maturation by mimicking GTP-Rab7. J. Cell Biol. 179: 965-980 [Abstract] [Full Text]  
• Cianciola, N. L., Crooks, D., Shah, A. H., Carlin, C. (2007). A Tyrosine-Based Signal Plays a Critical Role in the Targeting and Function of Adenovirus RID{alpha} Protein. J. Virol. 81: 10437-10450 [Abstract] [Full Text]  
• Delgado-Lopez, F., Horwitz, M. S. (2006). Adenovirus RID{alpha}{beta} Complex Inhibits Lipopolysaccharide Signaling without Altering TLR4 Cell Surface Expression.. J. Virol. 80: 6378-6386 [Abstract] [Full Text]  
• Iannello, A., Debbeche, O., Martin, E., Attalah, L. H., Samarani, S., Ahmad, A. (2006). Viral strategies for evading antiviral cellular immune responses of the host. J. Leukoc. Biol. 79: 16-35 [Abstract] [Full Text]  
• Chin, Y. R., Horwitz, M. S. (2005). Mechanism for Removal of Tumor Necrosis Factor Receptor 1 from the Cell Surface by the Adenovirus RID{alpha}/{beta} Complex. J. Virol. 79: 13606-13617 [Abstract] [Full Text]  
• Fessler, S. P., Chin, Y. R., Horwitz, M. S. (2004). Inhibition of Tumor Necrosis Factor (TNF) Signal Transduction by the Adenovirus Group C RID Complex Involves Downregulation of Surface Levels of TNF Receptor 1. J. Virol. 78: 13113-13121 [Abstract] [Full Text]  
• Lichtenstein, D. L., Doronin, K., Toth, K., Kuppuswamy, M., Wold, W. S. M., Tollefson, A. E. (2004). Adenovirus E3-6.7K Protein Is Required in Conjunction with the E3-RID Protein Complex for the Internalization and Degradation of TRAIL Receptor 2. J. Virol. 78: 12297-12307 [Abstract] [Full Text]  
• Hilgendorf, A., Lindberg, J., Ruzsics, Z., Honing, S., Elsing, A., Lofqvist, M., Engelmann, H., Burgert, H.-G. (2003). Two Distinct Transport Motifs in the Adenovirus E3/10.4-14.5 Proteins Act in Concert to Down-modulate Apoptosis Receptors and the Epidermal Growth Factor Receptor. J. Biol. Chem. 278: 51872-51884 [Abstract] [Full Text]  
• Zanardi, T. A., Yei, S., Lichtenstein, D. L., Tollefson, A. E., Wold, W. S. M. (2003). Distinct Domains in the Adenovirus E3 RID{alpha} Protein Are Required for Degradation of Fas and the Epidermal Growth Factor Receptor. J. Virol. 77: 11685-11696 [Abstract] [Full Text]  
• Fairley, J. A., Baillie, J., Bain, M., Sinclair, J. H. (2002). Human cytomegalovirus infection inhibits epidermal growth factor (EGF) signalling by targeting EGF receptors. J. Gen. Virol. 83: 2803-2810 [Abstract] [Full Text]  
• Lichtenstein, D. L., Krajcsi, P., Esteban, D. J., Tollefson, A. E., Wold, W. S. M. (2002). Adenovirus RID{beta} Subunit Contains a Tyrosine Residue That Is Critical for RID-Mediated Receptor Internalization and Inhibition of Fas- and TRAIL-Induced Apoptosis. J. Virol. 76: 11329-11342 [Abstract] [Full Text]  
• McNees, A. L., Garnett, C. T., Gooding, L. R. (2002). The Adenovirus E3 RID Complex Protects Some Cultured Human T and B Lymphocytes from Fas-Induced Apoptosis. J. Virol. 76: 9716-9723 [Abstract] [Full Text]  
• Tollefson, A. E., Toth, K., Doronin, K., Kuppuswamy, M., Doronina, O. A., Lichtenstein, D. L., Hermiston, T. W., Smith, C. A., Wold, W. S. M. (2001). Inhibition of TRAIL-Induced Apoptosis and Forced Internalization of TRAIL Receptor 1 by Adenovirus Proteins. J. Virol. 75: 8875-8887 [Abstract] [Full Text]  
• Elkon, K. B., Liu, C.-C., Gall, J. G., Trevejo, J., Marino, M. W., Abrahamsen, K. A., Song, X., Zhou, J.-L., Old, L. J., Crystal, R. G., Falck-Pedersen, E. (1997). Tumor necrosis factor alpha  plays a central role in immune-mediated clearance of adenoviral vectors. Proc. Natl. Acad. Sci. USA 94: 9814-9819 [Abstract] [Full Text]  
• Byrnes, A. P., MacLaren, R. E., Charlton, H. M. (1996). Immunological Instability of Persistent Adenovirus Vectors in the Brain: Peripheral Exposure to Vector Leads to Renewed Inflammation, Reduced Gene Expression, and Demyelination. J. Neurosci. 16: 3045-3055 [Abstract] [Full Text]  
• Benedict, C. A., Norris, P. S., Prigozy, T. I., Bodmer, J.-L., Mahr, J. A., Garnett, C. T., Martinon, F., Tschopp, J., Gooding, L. R., Ware, C. F. (2001). Three Adenovirus E3 Proteins Cooperate to Evade Apoptosis by Tumor Necrosis Factor-related Apoptosis-inducing Ligand Receptor-1 and -2. J. Biol. Chem. 276: 3270-3278 [Abstract] [Full Text]