This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hardwicke, M A Articles by Sandri-Goldin, R M Search for Related Content PubMed PubMed Citation Articles by Hardwicke, M A Articles by Sandri-Goldin, R M

 Previous Article  |  Next Article 

J Virol. 1994 August; 68(8): 4797-4810

The herpes simplex virus regulatory protein ICP27 contributes to the decrease in cellular mRNA levels during infection.

Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717-4025.

ABSTRACT

We have previously shown that the herpes simplex virus immediate-early regulatory protein ICP27 acts posttranscriptionally to affect mRNA processing (R. M. Sandri-Goldin and G. E. Mendoza, Genes Dev. 6:848-863, 1992). Specifically, in the presence of ICP27, spliced target mRNAs were decreased 5- to 10-fold in transfections with target genes containing a 5' or 3' intron. Here, we have investigated the effect of ICP27 during herpes simplex virus type 1 (HSV-1) infection on accumulation of spliced cellular mRNAs. ICP27 viral mutants have been shown to be defective in host shutoff (W. R. Sacks, C. C. Greene, D. P. Aschman, and P. A. Schaffer, J. Virol. 55:796-805, 1985). Therefore, we examined whether ICP27 could contribute to this complex process by decreasing cellular mRNA levels through its effects on host cell splicing. It was found that in infections with viral mutants defective in ICP27, the accumulated levels of three spliced host mRNAs were higher than those seen with wild-type HSV-1. The differences occurred posttranscriptionally as shown by nuclear runoff transcription assays. The stabilities of the spliced products during infection with wild-type or ICP27 mutant viruses were similar, and unspliced precursor mRNA for a viral spliced gene was detected in infections with wild-type HSV-1 but not in infections in which ICP27 was not expressed. These results suggest that the reduction in cellular mRNA levels and the accumulation of pre-mRNA are related and may be caused by an impairment in host cell splicing. These data further show that ICP27 is required for these effects to occur.

This article has been cited by other articles:

• Sedlackova, L., Perkins, K. D., Lengyel, J., Strain, A. K., van Santen, V. L., Rice, S. A. (2008). Herpes Simplex Virus Type 1 ICP27 Regulates Expression of a Variant, Secreted Form of Glycoprotein C by an Intron Retention Mechanism. J. Virol. 82: 7443-7455 [Abstract] [Full Text]  
• Minami, K., Tambe, Y., Watanabe, R., Isono, T., Haneda, M., Isobe, K.-i., Kobayashi, T., Hino, O., Okabe, H., Chano, T., Inoue, H. (2007). Suppression of Viral Replication by Stress-Inducible GADD34 Protein via the Mammalian Serine/Threonine Protein Kinase mTOR Pathway. J. Virol. 81: 11106-11115 [Abstract] [Full Text]  
• Corcoran, J. A., Hsu, W.-L., Smiley, J. R. (2006). Herpes Simplex Virus ICP27 Is Required for Virus-Induced Stabilization of the ARE-Containing IEX-1 mRNA Encoded by the Human IER3 Gene. J. Virol. 80: 9720-9729 [Abstract] [Full Text]  
• Pasieka, T. J., Baas, T., Carter, V. S., Proll, S. C., Katze, M. G., Leib, D. A. (2006). Functional genomic analysis of herpes simplex virus type 1 counteraction of the host innate response.. J. Virol. 80: 7600-7612 [Abstract] [Full Text]  
• Melchjorsen, J., Siren, J., Julkunen, I., Paludan, S. R., Matikainen, S. (2006). Induction of cytokine expression by herpes simplex virus in human monocyte-derived macrophages and dendritic cells is dependent on virus replication and is counteracted by ICP27 targeting NF-{kappa}B and IRF-3.. J. Gen. Virol. 87: 1099-1108 [Abstract] [Full Text]  
• Schwartz, J. A., Brittle, E. E., Reynolds, A. E., Enquist, L. W., Silverstein, S. J. (2006). UL54-Null Pseudorabies Virus Is Attenuated in Mice but Productively Infects Cells in Culture. J. Virol. 80: 769-784 [Abstract] [Full Text]  
• Pomeranz, L. E., Reynolds, A. E., Hengartner, C. J. (2005). Molecular Biology of Pseudorabies Virus: Impact on Neurovirology and Veterinary Medicine. Microbiol. Mol. Biol. Rev. 69: 462-500 [Abstract] [Full Text]  
• Feng, P., Everly, D. N. Jr., Read, G. S. (2005). mRNA Decay during Herpes Simplex Virus (HSV) Infections: Protein-Protein Interactions Involving the HSV Virion Host Shutoff Protein and Translation Factors eIF4H and eIF4A. J. Virol. 79: 9651-9664 [Abstract] [Full Text]  
• Chen, I-H. B., Li, L., Silva, L., Sandri-Goldin, R. M. (2005). ICP27 Recruits Aly/REF but Not TAP/NXF1 to Herpes Simplex Virus Type 1 Transcription Sites although TAP/NXF1 Is Required for ICP27 Export. J. Virol. 79: 3949-3961 [Abstract] [Full Text]  
• Ellison, K. S., Maranchuk, R. A., Mottet, K. L., Smiley, J. R. (2005). Control of VP16 Translation by the Herpes Simplex Virus Type 1 Immediate-Early Protein ICP27. J. Virol. 79: 4120-4131 [Abstract] [Full Text]  
• Malik, P., Blackbourn, D. J., Clements, J. B. (2004). The Evolutionarily Conserved Kaposi's Sarcoma-associated Herpesvirus ORF57 Protein Interacts with REF Protein and Acts as an RNA Export Factor. J. Biol. Chem. 279: 33001-33011 [Abstract] [Full Text]  
• Smiley, J. R. (2004). Herpes Simplex Virus Virion Host Shutoff Protein: Immune Evasion Mediated by a Viral RNase?. J. Virol. 78: 1063-1068 [Full Text]  
• Pearson, A., Knipe, D. M., Coen, D. M. (2004). ICP27 Selectively Regulates the Cytoplasmic Localization of a Subset of Viral Transcripts in Herpes Simplex Virus Type 1-Infected Cells. J. Virol. 78: 23-32 [Abstract] [Full Text]  
• Ruvolo, V., Sun, L., Howard, K., Sung, S., Delecluse, H.-J., Hammerschmidt, W., Swaminathan, S. (2004). Functional Analysis of Epstein-Barr Virus SM Protein: Identification of Amino Acids Essential for Structure, Transactivation, Splicing Inhibition, and Virion Production. J. Virol. 78: 340-352 [Abstract] [Full Text]  
• Perkins, K. D., Gregonis, J., Borge, S., Rice, S. A. (2003). Transactivation of a Viral Target Gene by Herpes Simplex Virus ICP27 Is Posttranscriptional and Does Not Require the Endogenous Promoter or Polyadenylation Site. J. Virol. 77: 9872-9884 [Abstract] [Full Text]  
• Taddeo, B., Esclatine, A., Roizman, B. (2002). The patterns of accumulation of cellular RNAs in cells infected with a wild-type and a mutant herpes simplex virus 1 lacking the virion host shutoff gene. Proc. Natl. Acad. Sci. USA 99: 17031-17036 [Abstract] [Full Text]  
• Chen, I-H. B., Sciabica, K. S., Sandri-Goldin, R. M. (2002). ICP27 Interacts with the RNA Export Factor Aly/REF To Direct Herpes Simplex Virus Type 1 Intronless mRNAs to the TAP Export Pathway. J. Virol. 76: 12877-12889 [Abstract] [Full Text]  
• Lengyel, J., Guy, C., Leong, V., Borge, S., Rice, S. A. (2002). Mapping of Functional Regions in the Amino-Terminal Portion of the Herpes Simplex Virus ICP27 Regulatory Protein: Importance of the Leucine-Rich Nuclear Export Signal and RGG Box RNA-Binding Domain. J. Virol. 76: 11866-11879 [Abstract] [Full Text]  
• Boyer, J. L., Swaminathan, S., Silverstein, S. J. (2002). The Epstein-Barr Virus SM Protein Is Functionally Similar to ICP27 from Herpes Simplex Virus in Viral Infections. J. Virol. 76: 9420-9433 [Abstract] [Full Text]  
• Everly, D. N. Jr., Feng, P., Mian, I. S., Read, G. S. (2002). mRNA Degradation by the Virion Host Shutoff (Vhs) Protein of Herpes Simplex Virus: Genetic and Biochemical Evidence that Vhs Is a Nuclease. J. Virol. 76: 8560-8571 [Abstract] [Full Text]  
• Ahmed, M., Fraser, N. W. (2001). Herpes Simplex Virus Type 1 2-Kilobase Latency-Associated Transcript Intron Associates with Ribosomal Proteins and Splicing Factors. J. Virol. 75: 12070-12080 [Abstract] [Full Text]  
• Preston, C. M., Harman, A. N., Nicholl, M. J. (2001). Activation of Interferon Response Factor-3 in Human Cells Infected with Herpes Simplex Virus Type 1 or Human Cytomegalovirus. J. Virol. 75: 8909-8916 [Abstract] [Full Text]  
• Ruvolo, V., Gupta, A. K., Swaminathan, S. (2001). Epstein-Barr Virus SM Protein Interacts with mRNA In Vivo and Mediates a Gene-Specific Increase in Cytoplasmic mRNA. J. Virol. 75: 6033-6041 [Abstract] [Full Text]  
• Lilley, C. E., Groutsi, F., Han, Z., Palmer, J. A., Anderson, P. N., Latchman, D. S., Coffin, R. S. (2001). Multiple Immediate-Early Gene-Deficient Herpes Simplex Virus Vectors Allowing Efficient Gene Delivery to Neurons in Culture and Widespread Gene Delivery to the Central Nervous System In Vivo. J. Virol. 75: 4343-4356 [Abstract] [Full Text]  
• Bryant, H. E., Wadd, S. E., Lamond, A. I., Silverstein, S. J., Clements, J. B. (2001). Herpes Simplex Virus IE63 (ICP27) Protein Interacts with Spliceosome-Associated Protein 145 and Inhibits Splicing prior to the First Catalytic Step. J. Virol. 75: 4376-4385 [Abstract] [Full Text]  
• Bryant, H. E., Matthews, D. A., Wadd, S., Scott, J. E., Kean, J., Graham, S., Russell, W. C., Clements, J. B. (2000). Interaction between Herpes Simplex Virus Type 1 IE63 Protein and Cellular Protein p32. J. Virol. 74: 11322-11328 [Abstract] [Full Text]  
• Stingley, S. W., Ramirez, J. J. G., Aguilar, S. A., Simmen, K., Sandri-Goldin, R. M., Ghazal, P., Wagner, E. K. (2000). Global Analysis of Herpes Simplex Virus Type 1 Transcription Using an Oligonucleotide-Based DNA Microarray. J. Virol. 74: 9916-9927 [Abstract] [Full Text]  
• Goodwin, D. J., Hall, K. T., Giles, M. S., Calderwood, M. A., Markham, A. F., Whitehouse, A. (2000). The carboxy terminus of the herpesvirus saimiri ORF 57 gene contains domains that are required for transactivation and transrepression. J. Gen. Virol. 81: 2253-2265 [Abstract] [Full Text]  
• Ellison, K. S., Rice, S. A., Verity, R., Smiley, J. R. (2000). Processing of alpha -Globin and ICP0 mRNA in Cells Infected with Herpes Simplex Virus Type 1 ICP27 Mutants. J. Virol. 74: 7307-7319 [Abstract] [Full Text]  
• Bunnell, S. M., Rice, S. A. (2000). The Conserved Carboxyl-Terminal Half of Herpes Simplex Virus Type 1 Regulatory Protein ICP27 Is Dispensable for Viral Growth in the Presence of Compensatory Mutations. J. Virol. 74: 7362-7374 [Abstract] [Full Text]  
• Soliman, T. M., Silverstein, S. J. (2000). Identification of an Export Control Sequence and a Requirement for the KH Domains in ICP27 from Herpes Simplex Virus Type 1. J. Virol. 74: 7600-7609 [Abstract] [Full Text]  
• Gale, M. Jr., Tan, S.-L., Katze, M. G. (2000). Translational Control of Viral Gene Expression in Eukaryotes. Microbiol. Mol. Biol. Rev. 64: 239-280 [Abstract] [Full Text]  
• Kirshner, J. R., Lukac, D. M., Chang, J., Ganem, D. (2000). Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 57 Encodes a Posttranscriptional Regulator with Multiple Distinct Activities. J. Virol. 74: 3586-3597 [Abstract] [Full Text]  
• Soliman, T. M., Silverstein, S. J. (2000). Herpesvirus mRNAs Are Sorted for Export via Crm1-Dependent and -Independent Pathways. J. Virol. 74: 2814-2825 [Abstract] [Full Text]  
• Cheung, P., Ellison, K. S., Verity, R., Smiley, J. R. (2000). Herpes Simplex Virus ICP27 Induces Cytoplasmic Accumulation of Unspliced Polyadenylated alpha -Globin Pre-mRNA in Infected HeLa Cells. J. Virol. 74: 2913-2919 [Abstract] [Full Text]  
• Gupta, A. K., Ruvolo, V., Patterson, C., Swaminathan, S. (2000). The Human Herpesvirus 8 Homolog of Epstein-Barr Virus SM Protein (KS-SM) Is a Posttranscriptional Activator of Gene Expression. J. Virol. 74: 1038-1044 [Abstract] [Full Text]  
• Aubert, M., O'Toole, J., Blaho, J. A. (1999). Induction and Prevention of Apoptosis in Human HEp-2 Cells by Herpes Simplex Virus Type 1. J. Virol. 73: 10359-10370 [Abstract] [Full Text]  
• Goodwin, D. J., Hall, K. T., Stevenson, A. J., Markham, A. F., Whitehouse, A. (1999). The Open Reading Frame 57 Gene Product of Herpesvirus Saimiri Shuttles between the Nucleus and Cytoplasm and Is Involved in Viral RNA Nuclear Export. J. Virol. 73: 10519-10524 [Abstract] [Full Text]  
• Everly, D. N. Jr., Read, G. S. (1999). Site-Directed Mutagenesis of the Virion Host Shutoff Gene (UL41) of Herpes Simplex Virus (HSV): Analysis of Functional Differences between HSV Type 1 (HSV-1) and HSV-2 Alleles. J. Virol. 73: 9117-9129 [Abstract] [Full Text]  
• Khodarev, N. N., Advani, S. J., Gupta, N., Roizman, B., Weichselbaum, R. R. (1999). Accumulation of specific RNAs encoding transcriptional factors and stress response proteins against a background of severe depletion of cellular RNAs in cells infected with herpes simplex virus 1. Proc. Natl. Acad. Sci. USA 96: 12062-12067 [Abstract] [Full Text]  
• Boyle, S. M., Ruvolo, V., Gupta, A. K., Swaminathan, S. (1999). Association with the Cellular Export Receptor CRM 1 Mediates Function and Intracellular Localization of Epstein-Barr Virus SM Protein, a Regulator of Gene Expression. J. Virol. 73: 6872-6881 [Abstract] [Full Text]  
• Bruni, R., Fineschi, B., Ogle, W. O., Roizman, B. (1999). A Novel Cellular Protein, p60, Interacting with both Herpes Simplex Virus 1 Regulatory Proteins ICP22 and ICP0 Is Modified in a Cell-Type-Specific Manner and Is Recruited to the Nucleus after Infection. J. Virol. 73: 3810-3817 [Abstract] [Full Text]  
• Cooper, M, Goodwin, D., Hall, K., Stevenson, A., Meredith, D., Markham, A., Whitehouse, A (1999). The gene product encoded by ORF 57 of herpesvirus saimiri regulates the redistribution of the splicing factor SC-35. J. Gen. Virol. 80: 1311-1316 [Abstract]  
• Zhi, Y., Sandri-Goldin, R. M. (1999). Analysis of the Phosphorylation Sites of Herpes Simplex Virus Type 1 Regulatory Protein ICP27. J. Virol. 73: 3246-3257 [Abstract] [Full Text]  
• Hann, L. E., Cook, W. J., Uprichard, S. L., Knipe, D. M., Coen, D. M. (1998). The Role of Herpes Simplex Virus ICP27 in the Regulation of UL24 Gene Expression by Differential Polyadenylation. J. Virol. 72: 7709-7714 [Abstract] [Full Text]  
• Petroski, M. D., Wagner, E. K. (1998). Purification and Characterization of a Cellular Protein That Binds to the Downstream Activation Sequence of the Strict Late UL38 Promoter of Herpes Simplex Virus Type 1. J. Virol. 72: 8181-8190 [Abstract] [Full Text]  
• Ruvolo, V., Wang, E., Boyle, S., Swaminathan, S. (1998). The Epstein-Barr virus nuclear protein SM is both a post-transcriptional inhibitor and activator of gene expression. Proc. Natl. Acad. Sci. USA 95: 8852-8857 [Abstract] [Full Text]  
• Sandri-Goldin, R. M. (1998). ICP27 mediates HSV RNA export by shuttling through a leucine-rich nuclear export signal and binding viral intronless RNAs through an RGG motif. Genes Dev. 12: 868-879 [Abstract] [Full Text]  
• Whitehouse, A., Cooper, M., Hall, K. T., Meredith, D. M. (1998). The Open Reading Frame (ORF) 50a Gene Product Regulates ORF 57 Gene Expression in Herpesvirus Saimiri. J. Virol. 72: 1967-1973 [Abstract] [Full Text]  
• Whitehouse, A., Cooper, M., Meredith, D. M. (1998). The Immediate-Early Gene Product Encoded by Open Reading Frame 57 of Herpesvirus Saimiri Modulates Gene Expression at a Posttranscriptional Level. J. Virol. 72: 857-861 [Abstract] [Full Text]