This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luban, J
Right arrow Articles by Goff, S P
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luban, J
Right arrow Articles by Goff, S P

 Previous Article  |  Next Article 

J Virol. 1994 June; 68(6): 3784-3793

Mutational analysis of cis-acting packaging signals in human immunodeficiency virus type 1 RNA.

J Luban and S P Goff

Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.

ABSTRACT

We previously identified blocks of sequence near the 5' end of the human immunodeficiency virus (HIV-1) genome which conferred on RNA the ability to bind specifically to the HIV-1 Gag polyprotein, Pr55gag (J. Luban and S. P. Goff, J. Virol. 65:3203-3212, 1991; R. Berkowitz, J. Luban, and S. P. Goff, J. Virol. 67:7190-7200, 1993). Here we report the use of an RNase protection assay to quantify the effect of deletion of these sequences on RNA packaging into virions. First, we demonstrated with wild-type HIV-1 sequences that in comparison with spliced viral RNA, full-length viral genomic RNA is enriched 20-fold in virions. A previously described mutation with deletion of sequences between the major splice donor and the first codon of gag (A. Lever, H. Gottlinger, W. Haseltine, and J. Sodroski, J. Virol. 63:4085-4087, 1989) disrupted these ratios such that different HIV-1 RNA forms were packaged in direct proportion to cytoplasmic concentrations. The effect of deletion mutations preceding and within gag coding sequence on packaging was then tested in competition with RNAs containing wild-type packaging sequences. Using this system, we were able to demonstrate significant effects on packaging of RNAs with mutations immediately preceding the first codon of gag. The greatest reduction in packaging was seen with RNAs lacking the first 40 nucleotides of gag coding sequence, although sequences more 3' had slight additional effects.

J Virol. 1994 June; 68(6): 3784-3793




This article has been cited by other articles:

  • Jouvenet, N., Simon, S. M., Bieniasz, P. D. (2009). Imaging the interaction of HIV-1 genomes and Gag during assembly of individual viral particles. Proc. Natl. Acad. Sci. USA 106: 19114-19119 [Abstract] [Full Text]  
  • Baig, T. T., Lanchy, J.-M., Lodmell, J. S. (2009). Randomization and In Vivo Selection Reveal a GGRG Motif Essential for Packaging Human Immunodeficiency Virus Type 2 RNA. J. Virol. 83: 802-810 [Abstract] [Full Text]  
  • Yu, E. T., Hawkins, A., Eaton, J., Fabris, D. (2008). MS3D structural elucidation of the HIV-1 packaging signal. Proc. Natl. Acad. Sci. USA 105: 12248-12253 [Abstract] [Full Text]  
  • Sinck, L., Richer, D., Howard, J., Alexander, M., Purcell, D. F.J., Marquet, R., Paillart, J.-C. (2007). In vitro dimerization of human immunodeficiency virus type 1 (HIV-1) spliced RNAs. RNA 13: 2141-2150 [Abstract] [Full Text]  
  • Laham-Karam, N., Bacharach, E. (2007). Transduction of Human Immunodeficiency Virus Type 1 Vectors Lacking Encapsidation and Dimerization Signals. J. Virol. 81: 10687-10698 [Abstract] [Full Text]  
  • Houzet, L., Paillart, J. C., Smagulova, F., Maurel, S., Morichaud, Z., Marquet, R., Mougel, M. (2007). HIV controls the selective packaging of genomic, spliced viral and cellular RNAs into virions through different mechanisms. Nucleic Acids Res 0: gkm153v1-10 [Abstract] [Full Text]  
  • Bjarnadottir, H., Gudmundsson, B., Gudnason, J., Jonsson, J. J. (2006). Encapsidation Determinants Located Downstream of the Major Splice Donor in the Maedi-Visna Virus Leader Region. J. Virol. 80: 11743-11755 [Abstract] [Full Text]  
  • Mark-Danieli, M., Laham, N., Kenan-Eichler, M., Castiel, A., Melamed, D., Landau, M., Bouvier, N. M., Evans, M. J., Bacharach, E. (2005). Single Point Mutations in the Zinc Finger Motifs of the Human Immunodeficiency Virus Type 1 Nucleocapsid Alter RNA Binding Specificities of the Gag Protein and Enhance Packaging and Infectivity. J. Virol. 79: 7756-7767 [Abstract] [Full Text]  
  • Ooms, M., Huthoff, H., Russell, R., Liang, C., Berkhout, B. (2004). A Riboswitch Regulates RNA Dimerization and Packaging in Human Immunodeficiency Virus Type 1 Virions. J. Virol. 78: 10814-10819 [Abstract] [Full Text]  
  • Roldan, A., Russell, R. S., Marchand, B., Gotte, M., Liang, C., Wainberg, M. A. (2004). In Vitro Identification and Characterization of an Early Complex Linking HIV-1 Genomic RNA Recognition and Pr55Gag Multimerization. J. Biol. Chem. 279: 39886-39894 [Abstract] [Full Text]  
  • Melamed, D., Mark-Danieli, M., Kenan-Eichler, M., Kraus, O., Castiel, A., Laham, N., Pupko, T., Glaser, F., Ben-Tal, N., Bacharach, E. (2004). The Conserved Carboxy Terminus of the Capsid Domain of Human Immunodeficiency Virus Type 1 Gag Protein Is Important for Virion Assembly and Release. J. Virol. 78: 9675-9688 [Abstract] [Full Text]  
  • Hill, M. K., Shehu-Xhilaga, M., Campbell, S. M., Poumbourios, P., Crowe, S. M., Mak, J. (2003). The Dimer Initiation Sequence Stem-Loop of Human Immunodeficiency Virus Type 1 Is Dispensable for Viral Replication in Peripheral Blood Mononuclear Cells. J. Virol. 77: 8329-8335 [Abstract] [Full Text]  
  • Brasey, A., Lopez-Lastra, M., Ohlmann, T., Beerens, N., Berkhout, B., Darlix, J.-L., Sonenberg, N. (2003). The Leader of Human Immunodeficiency Virus Type 1 Genomic RNA Harbors an Internal Ribosome Entry Segment That Is Active during the G2/M Phase of the Cell Cycle. J. Virol. 77: 3939-3949 [Abstract] [Full Text]  
  • Russell, R. S., Hu, J., Beriault, V., Mouland, A. J., Kleiman, L., Wainberg, M. A., Liang, C. (2002). Sequences Downstream of the 5' Splice Donor Site Are Required for both Packaging and Dimerization of Human Immunodeficiency Virus Type 1 RNA. J. Virol. 77: 84-96 [Abstract] [Full Text]  
  • Kemler, I., Barraza, R., Poeschla, E. M. (2002). Mapping the Encapsidation Determinants of Feline Immunodeficiency Virus. J. Virol. 76: 11889-11903 [Abstract] [Full Text]  
  • Clever, J. L., Miranda, D. Jr., Parslow, T. G. (2002). RNA Structure and Packaging Signals in the 5' Leader Region of the Human Immunodeficiency Virus Type 1 Genome. J. Virol. 76: 12381-12387 [Abstract] [Full Text]  
  • Shehu-Xhilaga, M., Hill, M., Marshall, J. A., Kappes, J., Crowe, S. M., Mak, J. (2002). The Conformation of the Mature Dimeric Human Immunodeficiency Virus Type 1 RNA Genome Requires Packaging of Pol Protein. J. Virol. 76: 4331-4340 [Abstract] [Full Text]  
  • Paillart, J.-C., Skripkin, E., Ehresmann, B., Ehresmann, C., Marquet, R. (2002). In Vitro Evidence for a Long Range Pseudoknot in the 5'-Untranslated and Matrix Coding Regions of HIV-1 Genomic RNA. J. Biol. Chem. 277: 5995-6004 [Abstract] [Full Text]  
  • Griffin, S. D. C., Allen, J. F., Lever, A. M. L. (2001). The Major Human Immunodeficiency Virus Type 2 (HIV-2) Packaging Signal Is Present on All HIV-2 RNA Species: Cotranslational RNA Encapsidation and Limitation of Gag Protein Confer Specificity. J. Virol. 75: 12058-12069 [Abstract] [Full Text]  
  • Shehu-Xhilaga, M., Kraeusslich, H. G., Pettit, S., Swanstrom, R., Lee, J. Y., Marshall, J. A., Crowe, S. M., Mak, J. (2001). Proteolytic Processing of the P2/Nucleocapsid Cleavage Site Is Critical for Human Immunodeficiency Virus Type 1 RNA Dimer Maturation. J. Virol. 75: 9156-9164 [Abstract] [Full Text]  
  • D’Costa, J., Brown, H. M., Kundra, P., Davis-Warren, A., Arya, S. K. (2001). Human immunodeficiency virus type 2 lentiviral vectors: packaging signal and splice donor in expression and encapsidation. J. Gen. Virol. 82: 425-434 [Abstract] [Full Text]  
  • Safak, M., Barrucco, R., Darbinyan, A., Okada, Y., Nagashima, K., Khalili, K. (2001). Interaction of JC Virus Agno Protein with T Antigen Modulates Transcription and Replication of the Viral Genome in Glial Cells. J. Virol. 75: 1476-1486 [Abstract] [Full Text]  
  • Mangeot, P.-E., Nègre, D., Dubois, B., Winter, A. J., Leissner, P., Mehtali, M., Kaiserlian, D., Cosset, F.-L., Darlix, J.-L. (2000). Development of Minimal Lentivirus Vectors Derived from Simian Immunodeficiency Virus (SIVmac251) and Their Use for Gene Transfer into Human Dendritic Cells. J. Virol. 74: 8307-8315 [Abstract] [Full Text]  
  • Jewell, N. A., Mansky, L. M. (2000). In the beginning: genome recognition, RNA encapsidation and the initiation of complex retrovirus assembly. J. Gen. Virol. 81: 1889-1899 [Full Text]  
  • Clever, J. L., Taplitz, R. A., Lochrie, M. A., Polisky, B., Parslow, T. G. (2000). A Heterologous, High-Affinity RNA Ligand for Human Immunodeficiency Virus Gag Protein Has RNA Packaging Activity. J. Virol. 74: 541-546 [Abstract] [Full Text]  
  • Banks, J. D., Kealoha, B. O., Linial, M. L. (1999). An MPsi -Containing Heterologous RNA, but Not env mRNA, Is Efficiently Packaged into Avian Retroviral Particles. J. Virol. 73: 8926-8933 [Abstract] [Full Text]  
  • Liang, C., Rong, L., Quan, Y., Laughrea, M., Kleiman, L., Wainberg, M. A. (1999). Mutations within Four Distinct Gag Proteins Are Required To Restore Replication of Human Immunodeficiency Virus Type 1 after Deletion Mutagenesis within the Dimerization Initiation Site. J. Virol. 73: 7014-7020 [Abstract] [Full Text]  
  • Liang, C., Rong, L., Cherry, E., Kleiman, L., Laughrea, M., Wainberg, M. A. (1999). Deletion Mutagenesis within the Dimerization Initiation Site of Human Immunodeficiency Virus Type 1 Results in Delayed Processing of the p2 Peptide from Precursor Proteins. J. Virol. 73: 6147-6151 [Abstract] [Full Text]  
  • Cui, Y., Iwakuma, T., Chang, L.-J. (1999). Contributions of Viral Splice Sites and cis-Regulatory Elements to Lentivirus Vector Function. J. Virol. 73: 6171-6176 [Abstract] [Full Text]  
  • Helga-Maria, C., Hammarskjöld, M.-L., Rekosh, D. (1999). An Intact TAR Element and Cytoplasmic Localization Are Necessary for Efficient Packaging of Human Immunodeficiency Virus Type 1 Genomic RNA. J. Virol. 73: 4127-4135 [Abstract] [Full Text]  
  • Liang, C., Rong, L., Laughrea, M., Kleiman, L., Wainberg, M. A. (1998). Compensatory Point Mutations in the Human Immunodeficiency Virus Type 1 Gag Region That Are Distal from Deletion Mutations in the Dimerization Initiation Site Can Restore Viral Replication. J. Virol. 72: 6629-6636 [Abstract] [Full Text]  
  • Bacharach, E., Goff, S. P. (1998). Binding of the Human Immunodeficiency Virus Type 1 Gag Protein to the Viral RNA Encapsidation Signal in the Yeast Three-Hybrid System. J. Virol. 72: 6944-6949 [Abstract] [Full Text]  
  • Kaye, J. F., Lever, A. M. L. (1998). Nonreciprocal Packaging of Human Immunodeficiency Virus Type 1 and Type 2 RNA: a Possible Role for the p2 Domain of Gag in RNA Encapsidation. J. Virol. 72: 5877-5885 [Abstract] [Full Text]  
  • Harrison, G. P., Miele, G., Hunter, E., Lever, A. M. L. (1998). Functional Analysis of the Core Human Immunodeficiency Virus Type 1 Packaging Signal in a Permissive Cell Line. J. Virol. 72: 5886-5896 [Abstract] [Full Text]  
  • St. Louis, D. C., Gotte, D., Sanders-Buell, E., Ritchey, D. W., Salminen, M. O., Carr, J. K., McCutchan, F. E. (1998). Infectious Molecular Clones with the Nonhomologous Dimer Initiation Sequences Found in Different Subtypes of Human Immunodeficiency Virus Type 1 Can Recombine and Initiate a Spreading Infection In Vitro. J. Virol. 72: 3991-3998 [Abstract] [Full Text]  
  • Mansky, L. M., Wisniewski, R. M. (1998). The Bovine Leukemia Virus Encapsidation Signal Is Composed of RNA Secondary Structures. J. Virol. 72: 3196-3204 [Abstract] [Full Text]  
  • Corbeau, P., Kraus, G., Wong-Staal, F. (1996). Efficient gene transfer by a human immunodeficiency virus type 1 (HIV-1)-derived vector utilizing a stable HIV packaging cell line. Proc. Natl. Acad. Sci. USA 93: 14070-14075 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»