JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kootstra, N A
Right arrow Articles by Schuitemaker, H
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kootstra, N A
Right arrow Articles by Schuitemaker, H

 Previous Article  |  Next Article 

J Virol. 1994 November; 68(11): 6967-6975

Interference of interleukin-10 with human immunodeficiency virus type 1 replication in primary monocyte-derived macrophages.

N A Kootstra, A van 't Wout, H G Huisman, F Miedema and H Schuitemaker

Department of Clinical Viro-Immunology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.

ABSTRACT

Previously we demonstrated an inhibitory effect of interleukin-4 (IL-4) on establishment of human immunodeficiency virus type 1 (HIV-1) infection in primary macrophages. The reported similarities between the biological effects of IL-4 and IL-10 prompted us to study the effect of IL-10 on HIV-1 replication. Treatment of primary macrophages with IL-10 resulted in inhibition of HIV-1 infection. This inhibitory effect was specific for macrophages, since IL-10 did not interfere with HIV-1 replication in primary T cells. Semiquantitative PCR analysis excluded an inhibitory effect of IL-10 on virus entry and reverse transcription. Effects of IL-10 on HIV-1 long terminal repeat-driven chloramphenicol acetyltransferase activity also could not be demonstrated in a transient expression system in primary derived macrophages. In agreement with this, Northern (RNA) blot analysis demonstrated equal amounts of viral RNA species irrespective of IL-10 treatment, also excluding an inhibitory effect on elongation of virus transcription. Monocyte-derived macrophages (MDM) treated with IL-10 after HIV-1 inoculation showed accumulation of apparently mature p24 protein suggestive of an inhibitory effect at the level of virus assembly. IL-10 treatment of MDM prior to HIV-1 inoculation did not result in accumulation of p24 protein. Immunoblot analysis indeed showed the absence of mature p24 and gp120 but accumulation of the Pr53 gag-encoded protein in HIV-1-inoculated, IL-10-pretreated MDM, suggesting an inhibitory effect at the level of protein processing. A combination of IL-4 and IL-10 resulted in a cumulative inhibitory effect on HIV-1 replication in MDM. The recent observation that in the course of HIV-1 infection a shift occurs in the production of IL-2/gamma interferon toward enhanced IL-4 and IL-10 production and the reported shift from preferential macrophage-tropic towards preferential T-cell-tropic HIV-1 variants with progression of disease suggest that cytokines have an important role in the in vivo regulation of HIV-1 tropism.

J Virol. 1994 November; 68(11): 6967-6975




This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1994 by the American Society for Microbiology. All rights reserved.