This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Miller, D G Articles by Miller, A D Search for Related Content PubMed PubMed Citation Articles by Miller, D G Articles by Miller, A D

Inhibitors of retrovirus infection are secreted by several hamster cell lines and are also present in hamster sera.

Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.

ABSTRACT

We have previously shown that Chinese hamster ovary (CHO) cells are resistant to infection by gibbon ape leukemia virus and amphotropic pseudotype retroviral vectors because of the secretion of factors that inhibit retrovirus infection. Such factors were not secreted by any mouse or human cell lines tested. Secretion of the inhibitors and resistance to infection are abrogated by treatment of CHO cells with the glycosylation inhibitor tunicamycin. Here we show that the inhibitory activities against gibbon ape leukemia virus and amphotropic viruses are partially separable and that glycosylation mutations in CHO cells mimic the effects of tunicamycin treatment. We find that several hamster cell lines derived from both Chinese and Syrian hamsters secrete inhibitors of retrovirus infection, showing that these inhibitors are not unique to the CHO cell line. Inhibitory factors are also present in the sera of Chinese and Syrian hamsters but were not detected in bovine serum. These results suggest the presence of specific factors that function to inhibit retrovirus infection in hamsters.

This article has been cited by other articles:

• Miller, A. D., Bergholz, U., Ziegler, M., Stocking, C. (2008). Identification of the Myelin Protein Plasmolipin as the Cell Entry Receptor for Mus caroli Endogenous Retrovirus. J. Virol. 82: 6862-6868 [Abstract] [Full Text]  
• Manel, N., Battini, J.-L., Sitbon, M. (2005). Human T Cell Leukemia Virus Envelope Binding and Virus Entry Are Mediated by Distinct Domains of the Glucose Transporter GLUT1. J. Biol. Chem. 280: 29025-29029 [Abstract] [Full Text]  
• Wang, W., Jobbagy, Z., Bird, T. H., Eiden, M. V., Anderson, W. B. (2005). Cell Signaling through the Protein Kinases cAMP-dependent Protein Kinase, Protein Kinase C{epsilon}, and RAF-1 Regulates Amphotropic Murine Leukemia Virus Envelope Protein-induced Syncytium Formation. J. Biol. Chem. 280: 16772-16783 [Abstract] [Full Text]  
• Van Hoeven, N. S., Miller, A. D. (2005). Improved Enzootic Nasal Tumor Virus Pseudotype Packaging Cell Lines Reveal Virus Entry Requirements in Addition to the Primary Receptor Hyal2. J. Virol. 79: 87-94 [Abstract] [Full Text]  
• Feldman, S. A, Farrell, K. B., Murthy, R. K., Russ, J. L., Eiden, M. V. (2004). Identification of an Extracellular Domain within the Human PiT2 Receptor That Is Required for Amphotropic Murine Leukemia Virus Binding. J. Virol. 78: 595-602 [Abstract] [Full Text]  
• Shikova-Lekova, E., Lindemann, D., Pietschmann, T., Juretzek, T., Rudolph, W., Herchenroder, O., Gelderblom, H. R., Rethwilm, A. (2003). Replication-Competent Hybrids between Murine Leukemia Virus and Foamy Virus. J. Virol. 77: 7677-7681 [Abstract] [Full Text]  
• Jones, K. S., Nath, M., Petrow-Sadowski, C., Baines, A. C., Dambach, M., Huang, Y., Ruscetti, F. W. (2002). Similar Regulation of Cell Surface Human T-Cell Leukemia Virus Type 1 (HTLV-1) Surface Binding Proteins in Cells Highly and Poorly Transduced by HTLV-1-Pseudotyped Virions. J. Virol. 76: 12723-12734 [Abstract] [Full Text]  
• Qi, A.-D., Zambon, A. C., Insel, P. A., Nicholas, R. A. (2001). An Arginine/Glutamine Difference at the Juxtaposition of Transmembrane Domain 6 and the Third Extracellular Loop Contributes to the Markedly Different Nucleotide Selectivities of Human and Canine P2Y11 Receptors. Mol. Pharmacol. 60: 1375-1382 [Abstract] [Full Text]  
• Salaün, C., Rodrigues, P., Heard, J. M. (2001). Transmembrane Topology of PiT-2, a Phosphate Transporter-Retrovirus Receptor. J. Virol. 75: 5584-5592 [Abstract] [Full Text]  
• Tailor, C. S., Nouri, A., Kabat, D. (2000). Cellular and Species Resistance to Murine Amphotropic, Gibbon Ape, and Feline Subgroup C Leukemia Viruses Is Strongly Influenced by Receptor Expression Levels and by Receptor Masking Mechanisms. J. Virol. 74: 9797-9801 [Abstract] [Full Text]  
• Marin, M., Tailor, C. S., Nouri, A., Kabat, D. (2000). Sodium-Dependent Neutral Amino Acid Transporter Type 1 Is an Auxiliary Receptor for Baboon Endogenous Retrovirus. J. Virol. 74: 8085-8093 [Abstract] [Full Text]  
• Chaudry, G. J., Farrell, K. B., Ting, Y.-T., Schmitz, C., S. Lie, Y., Petropoulos, C. J., Eiden, M. V. (1999). Gibbon Ape Leukemia Virus Receptor Functions of Type III Phosphate Transporters from CHOK1 Cells Are Disrupted by Two Distinct Mechanisms. J. Virol. 73: 2916-2920 [Abstract] [Full Text]  
• Battini, J.-L., Rasko, J. E. J., Miller, A. D. (1999). A human cell-surface receptor for xenotropic and polytropic murine leukemia viruses: Possible role in G protein-coupled signal transduction. Proc. Natl. Acad. Sci. USA 96: 1385-1390 [Abstract] [Full Text]  
• Pushkarsky, T., Zybarth, G., Dubrovsky, L., Yurchenko, V., Tang, H., Guo, H., Toole, B., Sherry, B., Bukrinsky, M. (2001). CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin A. Proc. Natl. Acad. Sci. USA 98: 6360-6365 [Abstract] [Full Text]