This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Caggana, M Articles by Ramsingh, A Search for Related Content PubMed PubMed Citation Articles by Caggana, M Articles by Ramsingh, A

 Previous Article  |  Next Article 

J Virol. 1993 August; 67(8): 4797-4803

Identification of a single amino acid residue in the capsid protein VP1 of coxsackievirus B4 that determines the virulent phenotype.

Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.

ABSTRACT

To identify the molecular determinants of virulence for coxsackievirus B4, a panel of recombinant, chimeric viruses were constructed from cDNA clones of a virulent virus, CB4-V, and a nonvirulent virus, CB4-P. Initial studies mapped a major determinant of virulence to the 5' end of the viral genome, which contained the 5' untranslated and the P1 regions (A. Ramsingh, A. Hixson, B. Duceman, and J. Slack, J. Virol. 64:3078-3081, 1990). To determine whether the 5' untranslated region contributed to the virulent phenotype, a chimeric virus (vCB403) containing this region of the virulent virus on an avirulent background was tested in mice. The vCB403 construct displayed a phenotype similar to that of CB4-P, suggesting that the 5' untranslated region did not significantly contribute to virulence. Analysis of the sequence data of the P1 regions of both CB4-V and CB4-P revealed five mutations that resulted in amino acid substitutions in VP1, VP2, and VP4 (A. Ramsingh, H. Araki, S. Bryant, and A. Hixson, Virus Res. 23:281-292, 1992). Analysis of individual mutations in both VP1 and VP2 revealed that a single residue (Thr-129 of VP1) determined the virulent phenotype.

This article has been cited by other articles:

• Chehadeh, W., Lobert, P.-E., Sauter, P., Goffard, A., Lucas, B., Weill, J., Vantyghem, M.-C., Alm, G., Pigny, P., Hober, D. (2005). Viral Protein VP4 Is a Target of Human Antibodies Enhancing Coxsackievirus B4- and B3-Induced Synthesis of Alpha Interferon. J. Virol. 79: 13882-13891 [Abstract] [Full Text]  
• Dan, M., Chantler, J. K. (2005). A Genetically Engineered Attenuated Coxsackievirus B3 Strain Protects Mice against Lethal Infection. J. Virol. 79: 9285-9295 [Abstract] [Full Text]  
• Harvala, H., Kalimo, H., Bergelson, J., Stanway, G., Hyypia, T. (2005). Tissue tropism of recombinant coxsackieviruses in an adult mouse model. J. Gen. Virol. 86: 1897-1907 [Abstract] [Full Text]  
• Serreze, D. V., Wasserfall, C., Ottendorfer, E. W., Stalvey, M., Pierce, M. A., Gauntt, C., O'Donnell, B., Flanagan, J. B., Campbell-Thompson, M., Ellis, T. M., Atkinson, M. A. (2005). Diabetes Acceleration or Prevention by a Coxsackievirus B4 Infection: Critical Requirements for both Interleukin-4 and Gamma Interferon. J. Virol. 79: 1045-1052 [Abstract] [Full Text]  
• Stadnick, E., Dan, M., Sadeghi, A., Chantler, J. K. (2004). Attenuating Mutations in Coxsackievirus B3 Map to a Conformational Epitope That Comprises the Puff Region of VP2 and the Knob of VP3. J. Virol. 78: 13987-14002 [Abstract] [Full Text]  
• Ostrowski, S. E., Reilly, A. A., Collins, D. N., Ramsingh, A. I. (2004). Progression or Resolution of Coxsackievirus B4-Induced Pancreatitis: a Genomic Analysis. J. Virol. 78: 8229-8237 [Abstract] [Full Text]  
• Tam, P. E., Weber-Sanders, M. L., Messner, R. P. (2003). Multiple Viral Determinants Mediate Myopathogenicity in Coxsackievirus B1-Induced Chronic Inflammatory Myopathy. J. Virol. 77: 11849-11854 [Abstract] [Full Text]  
• Potvin, D. M., Metzger, D. W., Lee, W. T., Collins, D. N., Ramsingh, A. I. (2003). Exogenous Interleukin-12 Protects against Lethal Infection with Coxsackievirus B4. J. Virol. 77: 8272-8279 [Abstract] [Full Text]  
• Tracy, S., Drescher, K. M., Chapman, N. M., Kim, K.-S., Carson, S. D., Pirruccello, S., Lane, P. H., Romero, J. R., Leser, J. S. (2002). Toward Testing the Hypothesis that Group B Coxsackieviruses (CVB) Trigger Insulin-Dependent Diabetes: Inoculating Nonobese Diabetic Mice with CVB Markedly Lowers Diabetes Incidence. J. Virol. 76: 12097-12111 [Abstract] [Full Text]  
• Harvala, H., Kalimo, H., Dahllund, L., Santti, J., Hughes, P., Hyypia, T., Stanway, G. (2002). Mapping of tissue tropism determinants in coxsackievirus genomes. J. Gen. Virol. 83: 1697-1706 [Abstract] [Full Text]  
• Brandt, M., Yao, K., Liu, M., Heckert, R. A., Vakharia, V. N. (2001). Molecular Determinants of Virulence, Cell Tropism, and Pathogenic Phenotype of Infectious Bursal Disease Virus. J. Virol. 75: 11974-11982 [Abstract] [Full Text]  
• McMinn, P., Lindsay, K., Perera, D., Chan, H. M., Chan, K. P., Cardosa, M. J. (2001). Phylogenetic Analysis of Enterovirus 71 Strains Isolated during Linked Epidemics in Malaysia, Singapore, and Western Australia. J. Virol. 75: 7732-7738 [Abstract] [Full Text]  
• Dunn, J. J., Chapman, N. M., Tracy, S., Romero, J. R. (2000). Genomic Determinants of Cardiovirulence in Coxsackievirus B3 Clinical Isolates: Localization to the 5' Nontranslated Region. J. Virol. 74: 4787-4794 [Abstract] [Full Text]  
• Mulders, M. N., Salminen, M., Kalkkinen, N., Hovi, T. (2000). Molecular epidemiology of coxsackievirus B4 and disclosure of the correct VP1/2Apro cleavage site: evidence for high genomic diversity and long-term endemicity of distinct genotypes. J. Gen. Virol. 81: 803-812 [Abstract] [Full Text]  
• Kanno, T., Mackay, D., Inoue, T., Wilsden, G., Yamakawa, M., Yamazoe, R., Yamaguchi, S., Shirai, J., Kitching, P., Murakami, Y. (1999). Mapping the Genetic Determinants of Pathogenicity and Plaque Phenotype in Swine Vesicular Disease Virus. J. Virol. 73: 2710-2716 [Abstract] [Full Text]  
• Ramsingh, A. I., Lee, W. T., Collins, D. N., Armstrong, L. E. (1999). T Cells Contribute to Disease Severity during Coxsackievirus B4 Infection. J. Virol. 73: 3080-3086 [Abstract] [Full Text]  
• Adami, C., Pritchard, A. E., Knauf, T., Luo, M., Lipton, H. L. (1998). A Determinant for Central Nervous System Persistence Localized in the Capsid of Theiler's Murine Encephalomyelitis Virus by Using Recombinant Viruses. J. Virol. 72: 1662-1665 [Abstract] [Full Text]  
• Muir, P., Kammerer, U., Korn, K., Mulders, M. N., Poyry, T., Weissbrich, B., Kandolf, R., Cleator, G. M., van Loon, A. M. (1998). Molecular Typing of Enteroviruses: Current Status and Future Requirements. Clin. Microbiol. Rev. 11: 202-227 [Abstract] [Full Text]