This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moore, J P Articles by Durda, P J Search for Related Content PubMed PubMed Citation Articles by Moore, J P Articles by Durda, P J

Immunochemical analysis of the gp120 surface glycoprotein of human immunodeficiency virus type 1: probing the structure of the C4 and V4 domains and the interaction of the C4 domain with the V3 loop.

Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016.

ABSTRACT

We have probed the structure of the C4 and V3 domains of human immunodeficiency virus type 1 gp120 by immunochemical techniques. Monoclonal antibodies (MAbs) recognizing an exposed gp120 sequence, (E/K)VGKAMYAPP, in C4 were differentially sensitive to denaturation of gp120, implying a conformational component to some of the epitopes. The MAbs recognizing conformation-sensitive C4 structures failed to bind to a gp120 mutant with an alteration in the sequence of the V3 loop, and their binding to gp120 was inhibited by both V3 and C4 MAbs. This implies an interaction between the V3 and C4 regions of gp120, which is supported by the observation that the binding of some MAbs to the V3 loop was often enhanced by amino acid changes in an around the C4 region.

This article has been cited by other articles:

• Tasca, S., Ho, S.-H., Cheng-Mayer, C. (2008). R5X4 Viruses Are Evolutionary, Functional, and Antigenic Intermediates in the Pathway of a Simian-Human Immunodeficiency Virus Coreceptor Switch. J. Virol. 82: 7089-7099 [Abstract] [Full Text]  
• Heap, C. J., Wang, Y., Pinheiro, T. J. T., Reading, S. A., Jennings, K. R., Dimmock, N. J. (2005). Analysis of a 17-amino acid residue, virus-neutralizing microantibody. J. Gen. Virol. 86: 1791-1800 [Abstract] [Full Text]  
• Gorny, M. K., Stamatatos, L., Volsky, B., Revesz, K., Williams, C., Wang, X.-H., Cohen, S., Staudinger, R., Zolla-Pazner, S. (2005). Identification of a New Quaternary Neutralizing Epitope on Human Immunodeficiency Virus Type 1 Virus Particles. J. Virol. 79: 5232-5237 [Abstract] [Full Text]  
• Kuhmann, S. E., Pugach, P., Kunstman, K. J., Taylor, J., Stanfield, R. L., Snyder, A., Strizki, J. M., Riley, J., Baroudy, B. M., Wilson, I. A., Korber, B. T., Wolinsky, S. M., Moore, J. P. (2004). Genetic and Phenotypic Analyses of Human Immunodeficiency Virus Type 1 Escape from a Small-Molecule CCR5 Inhibitor. J. Virol. 78: 2790-2807 [Abstract] [Full Text]  
• Poumbourios, P., Maerz, A. L., Drummer, H. E. (2003). Functional Evolution of the HIV-1 Envelope Glycoprotein 120 Association Site of Glycoprotein 41. J. Biol. Chem. 278: 42149-42160 [Abstract] [Full Text]  
• Zwick, M. B., Kelleher, R., Jensen, R., Labrijn, A. F., Wang, M., Quinnan, G. V. Jr., Parren, P. W. H. I., Burton, D. R. (2003). A Novel Human Antibody against Human Immunodeficiency Virus Type 1 gp120 Is V1, V2, and V3 Loop Dependent and Helps Delimit the Epitope of the Broadly Neutralizing Antibody Immunoglobulin G1 b12. J. Virol. 77: 6965-6978 [Abstract] [Full Text]  
• Hoffman, N. G., Seillier-Moiseiwitsch, F., Ahn, J., Walker, J. M., Swanstrom, R. (2002). Variability in the Human Immunodeficiency Virus Type 1 gp120 Env Protein Linked to Phenotype-Associated Changes in the V3 Loop. J. Virol. 76: 3852-3864 [Abstract] [Full Text]  
• Zhu, C.-B., Zhu, L., Holz-Smith, S., Matthews, T. J., Chen, C. H. (2001). The role of the third beta strand in gp120 conformation and neutralization sensitivity of the HIV-1 primary isolate DH012. Proc. Natl. Acad. Sci. USA 10.1073/pnas.261359098v1 [Abstract] [Full Text]  
• Moore, J. P., Parren, P. W. H. I., Burton, D. R. (2001). Genetic Subtypes, Humoral Immunity, and Human Immunodeficiency Virus Type 1 Vaccine Development. J. Virol. 75: 5721-5729 [Full Text]  
• Malenbaum, S. E., Yang, D., Cavacini, L., Posner, M., Robinson, J., Cheng-Mayer, C. (2000). The N-Terminal V3 Loop Glycan Modulates the Interaction of Clade A and B Human Immunodeficiency Virus Type 1 Envelopes with CD4 and Chemokine Receptors. J. Virol. 74: 11008-11016 [Abstract] [Full Text]  
• Sanders, R. W., Schiffner, L., Master, A., Kajumo, F., Guo, Y., Dragic, T., Moore, J. P., Binley, J. M. (2000). Variable-Loop-Deleted Variants of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Can Be Stabilized by an Intermolecular Disulfide Bond between the gp120 and gp41 Subunits. J. Virol. 74: 5091-5100 [Abstract] [Full Text]  
• Binley, J. M., Sanders, R. W., Clas, B., Schuelke, N., Master, A., Guo, Y., Kajumo, F., Anselma, D. J., Maddon, P. J., Olson, W. C., Moore, J. P. (2000). A Recombinant Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Complex Stabilized by an Intermolecular Disulfide Bond between the gp120 and gp41 Subunits Is an Antigenic Mimic of the Trimeric Virion-Associated Structure. J. Virol. 74: 627-643 [Abstract] [Full Text]  
• HOWIE, S. E. M., FERNANDES, M. L., HESLOP, I., HEWSON, T. J., COTTON, G. J., MOORE, M. J., INNES, D., RAMAGE, R., HARRISON, D. J. (1999). A functional, discontinuous HIV-1 gp120 C3/C4 domain-derived, branched, synthetic peptide that binds to CD4 and inhibits MIP-1{alpha} chemokine binding. FASEB J. 13: 503-511 [Abstract] [Full Text]  
• Misse, D., Cerutti, M., Schmidt, I., Jansen, A., Devauchelle, G., Jansen, F., Veas, F. (1998). Dissociation of the CD4 and CXCR4 Binding Properties of Human Immunodeficiency Virus Type 1 gp120 by Deletion of the First Putative Alpha-Helical Conserved Structure. J. Virol. 72: 7280-7288 [Abstract] [Full Text]  
• Kmieciak, D., Wasik, T. J., Teppler, H., Pientka, J., Hsu, S. H., Takahashi, H., Okumura, K., Kaneko, Y., Kozbor, D. (1998). The Effect of Deletion of the V3 Loop of gp120 on Cytotoxic T Cell Responses and HIV gp120-Mediated Pathogenesis. J. Immunol. 160: 5676-5683 [Abstract] [Full Text]  
• Wang, W.-K., Dudek, T., Zhao, Y.-J., Brumblay, H. G., Essex, M., Lee, T.-H. (1998). CCR5 coreceptor utilization involves a highly conserved arginine residue of HIV type 1 gp120. Proc. Natl. Acad. Sci. USA 95: 5740-5745 [Abstract] [Full Text]  
• Karray, S., Zouali, M. (1997). Identification of the B cell superantigen-binding site of HIV-1 gp120. Proc. Natl. Acad. Sci. USA 94: 1356-1360 [Abstract] [Full Text]  
• Freed, E. O., Martin, M. A. (1995). The Role of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins in Virus Infection. J. Biol. Chem. 270: 23883-23886 [Full Text]  
• Zhu, C.-B., Zhu, L., Holz-Smith, S., Matthews, T. J., Chen, C. H. (2001). The role of the third beta strand in gp120 conformation and neutralization sensitivity of the HIV-1 primary isolate DH012. Proc. Natl. Acad. Sci. USA 98: 15227-15232 [Abstract] [Full Text]