This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lombardi, S Articles by Bendinelli, M Search for Related Content PubMed PubMed Citation Articles by Lombardi, S Articles by Bendinelli, M

 Previous Article  |  Next Article 

J Virol. 1993 August; 67(8): 4742-4749

Identification of a linear neutralization site within the third variable region of the feline immunodeficiency virus envelope.

Department of Biomedicine, University of Pisa, Italy.

ABSTRACT

Synthetic peptides have been used to map linear B-cell epitopes of the third variable (V3) region of the feline immunodeficiency virus (FIV) external membrane glycoprotein gp120. The analysis of sera from naturally and experimentally FIV-infected cats by Pepscan and enzyme immunoassay with four partially overlapping peptides evidenced three antibody-binding domains, two of which mapped in the carboxyl-terminal half of V3. In particular, the V3.3 sequence (Gly-392-Phe-413) turned out to be important for in vitro neutralization of the virus in that the peptide inhibited the FIV-neutralizing activity of pooled immune cat sera, and on the other hand, cat sera raised against this peptide effectively neutralized FIV infectivity for Crandell feline kidney cells.

This article has been cited by other articles:

• Sundstrom, M., White, R. L., de Parseval, A., Sastry, K. J., Morris, G., Grant, C. K., Elder, J. H. (2008). Mapping of the CXCR4 Binding Site within Variable Region 3 of the Feline Immunodeficiency Virus Surface Glycoprotein. J. Virol. 82: 9134-9142 [Abstract] [Full Text]  
• Giannecchini, S., Pistello, M., Isola, P., Matteucci, D., Mazzetti, P., Freer, G., Bendinelli, M. (2007). Role of Env in Resistance of Feline Immunodeficiency Virus (FIV)-Infected Cats to Superinfection by a Second FIV Strain as Determined by Using a Chimeric Virus. J. Virol. 81: 10474-10485 [Abstract] [Full Text]  
• de Parseval, A., Grant, C. K., Sastry, K. J., Elder, J. H. (2006). Sequential CD134-CXCR4 Interactions in Feline Immunodeficiency Virus (FIV): Soluble CD134 Activates FIV Env for CXCR4-Dependent Entry and Reveals a Cryptic Neutralization Epitope. J. Virol. 80: 3088-3091 [Abstract] [Full Text]  
• Huisman, W., Schrauwen, E. J. A., Pas, S. D., Karlas, J. A., Rimmelzwaan, G. F., Osterhaus, A. D. M. E. (2004). Antibodies specific for hypervariable regions 3 to 5 of the feline immunodeficiency virus envelope glycoprotein are not solely responsible for vaccine-induced acceleration of challenge infection in cats. J. Gen. Virol. 85: 1833-1841 [Abstract] [Full Text]  
• Hötzel, I., Cheevers, W. P. (2001). Conservation of Human Immunodeficiency Virus Type 1 gp120 Inner-Domain Sequences in Lentivirus and Type A and B Retrovirus Envelope Surface Glycoproteins. J. Virol. 75: 2014-2018 [Abstract] [Full Text]  
• Bertoni, G., Hertig, C., Zahno, M.-L., Vogt, H.-R., Dufour, S., Cordano, P., Peterhans, E., Cheevers, W. P., Sonigo, P., Pancino, G. (2000). B-cell epitopes of the envelope glycoprotein of caprine arthritis-encephalitis virus and antibody response in infected goats. J. Gen. Virol. 81: 2929-2940 [Abstract] [Full Text]  
• Hosie, M. J., Dunsford, T., Klein, D., Willett, B. J., Cannon, C., Osborne, R., MacDonald, J., Spibey, N., Mackay, N., Jarrett, O., Neil, J. C. (2000). Vaccination with Inactivated Virus but Not Viral DNA Reduces Virus Load following Challenge with a Heterologous and Virulent Isolate of Feline Immunodeficiency Virus. J. Virol. 74: 9403-9411 [Abstract] [Full Text]  
• Vahlenkamp, T. W., De Ronde, A., Schuurman, N. N. M. P., van Vliet, A. L. W., van Drunen, J., Horzinek, M. C., Egberink, H. F. (1999). Envelope gene sequences encoding variable regions 3 and 4 are involved in macrophage tropism of feline immunodeficiency virus. J. Gen. Virol. 80: 2639-2646 [Abstract] [Full Text]  
• Chiarantini, L., Matteucci, D., Pistello, M., Mancini, U., Mazzetti, P., Massi, C., Giannecchini, S., Lonetti, I., Magnani, M., Bendinelli, M. (1998). AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Homologous Erythrocytes as a Delivery System for Preferential Immunization with Putative Protective Antigens. CVI 5: 235-241 [Abstract] [Full Text]  
• del Mauro, D., Matteucci, D., Giannecchini, S., Maggi, F., Pistello, M., Bendinelli, M. (1998). Autologous and Heterologous Neutralization Analyses of Primary Feline Immunodeficiency Virus Isolates. J. Virol. 72: 2199-2207 [Abstract] [Full Text]  
• Richardson, J., Moraillon, A., Crespeau, F., Baud, S., Sonigo, P., Pancino, G. (1998). Delayed Infection after Immunization with a Peptide from the Transmembrane Glycoprotein of the Feline Immunodeficiency Virus. J. Virol. 72: 2406-2415 [Abstract] [Full Text]