This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Luckow, V A Articles by Olins, P O Search for Related Content PubMed PubMed Citation Articles by Luckow, V A Articles by Olins, P O

 Previous Article  |  Next Article 

J Virol. 1993 August; 67(8): 4566-4579

Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli.

Cellular and Molecular Biochemistry, Monsanto Corporate Research, Chesterfield, Missouri 63198.

ABSTRACT

The construction and purification of recombinant baculovirus vectors for the expression of foreign genes in insect cells by standard transfection and plaque assay methods can take as long as 4 to 6 weeks. This period can be reduced to several days by using a novel baculovirus shuttle vector (bacmid) that can replicate in Escherichia coli as a plasmid and can infect susceptible lepidopteran insect cells. The bacmid is a recombinant virus that contains a mini-F replicon, a kanamycin resistance marker, and attTn7, the target site for the bacterial transposon Tn7. Expression cassettes comprising a baculovirus promoter driving expression of a foreign gene that is flanked by the left and right ends of Tn7 can transpose to the target bacmid in E. coli when Tn7 transposition functions are provided in trans by a helper plasmid. The foreign gene is expressed when the resulting composite bacmid is introduced into insect cells.

This article has been cited by other articles:

• Yin, F., Wang, M., Tan, Y., Deng, F., Vlak, J. M., Hu, Z., Wang, H. (2008). A Functional F Analogue of Autographa californica Nucleopolyhedrovirus GP64 from the Agrotis segetum Granulovirus. J. Virol. 82: 8922-8926 [Abstract] [Full Text]  
• Perkins, E. M., Anacker, D., Davis, A., Sankar, V., Ambinder, R. F., Desai, P. (2008). Small Capsid Protein pORF65 Is Essential for Assembly of Kaposi's Sarcoma-Associated Herpesvirus Capsids. J. Virol. 82: 7201-7211 [Abstract] [Full Text]  
• Li, Y., Guarino, L. A. (2008). Roles of LEF-4 and PTP/BVP RNA Triphosphatases in Processing of Baculovirus Late mRNAs. J. Virol. 82: 5573-5583 [Abstract] [Full Text]  
• Zwart, M. P., Erro, E., van Oers, M. M., de Visser, J. A. G. M., Vlak, J. M. (2008). Low multiplicity of infection in vivo results in purifying selection against baculovirus deletion mutants. J. Gen. Virol. 89: 1220-1224 [Abstract] [Full Text]  
• Wang, Y., Wang, Q., Liang, C., Song, J., Li, N., Shi, H., Chen, X. (2008). Autographa californica Multiple Nucleopolyhedrovirus Nucleocapsid Protein BV/ODV-C42 Mediates the Nuclear Entry of P78/83. J. Virol. 82: 4554-4561 [Abstract] [Full Text]  
• Wang, Q., Wang, Y., Liang, C., Song, J., Chen, X. (2008). Identification of a Hydrophobic Domain of HA2 Essential to Morphogenesis of Helicoverpa armigera Nucleopolyhedrovirus. J. Virol. 82: 4072-4081 [Abstract] [Full Text]  
• Li, Z., Blissard, G. W. (2008). Functional Analysis of the Transmembrane (TM) Domain of the Autographa californica Multicapsid Nucleopolyhedrovirus GP64 Protein: Substitution of Heterologous TM Domains. J. Virol. 82: 3329-3341 [Abstract] [Full Text]  
• Ge, J.-Q., Yang, Z.-N., Tang, X.-D., Xu, H.-J., Hong, J., Chen, J.-G., Zhang, C.-X. (2008). Characterization of a nucleopolyhedrovirus with a deletion of the baculovirus core gene Bm67. J. Gen. Virol. 89: 766-774 [Abstract] [Full Text]  
• Westenberg, M., Vlak, J. M. (2008). GP64 of group I nucleopolyhedroviruses cannot readily rescue infectivity of group II f-null nucleopolyhedroviruses. J. Gen. Virol. 89: 424-431 [Abstract] [Full Text]  
• Hwang, J.-S., Kregler, O., Schilf, R., Bannert, N., Drach, J. C., Townsend, L. B., Bogner, E. (2007). Identification of Acetylated, Tetrahalogenated Benzimidazole D-Ribonucleosides with Enhanced Activity against Human Cytomegalovirus. J. Virol. 81: 11604-11611 [Abstract] [Full Text]  
• Fang, M., Dai, X., Theilmann, D. A. (2007). Autographa californica Multiple Nucleopolyhedrovirus EXON0 (ORF141) Is Required for Efficient Egress of Nucleocapsids from the Nucleus. J. Virol. 81: 9859-9869 [Abstract] [Full Text]  
• Wu, W., Lin, T., Pan, L., Yu, M., Li, Z., Pang, Y., Yang, K. (2006). Autographa californica Multiple Nucleopolyhedrovirus Nucleocapsid Assembly Is Interrupted upon Deletion of the 38K Gene. J. Virol. 80: 11475-11485 [Abstract] [Full Text]  
• Shen, W., Hanley-Bowdoin, L. (2006). Geminivirus Infection Up-Regulates the Expression of Two Arabidopsis Protein Kinases Related to Yeast SNF1- and Mammalian AMPK-Activating Kinases. Plant Physiol. 142: 1642-1655 [Abstract] [Full Text]  
• Visch, H.-J., Koopman, W. J. H., Zeegers, D., van Emst-de Vries, S. E., van Kuppeveld, F. J. M., van den Heuvel, L. W. P. J., Smeitink, J. A. M., Willems, P. H. G. M. (2006). Ca2+-mobilizing agonists increase mitochondrial ATP production to accelerate cytosolic Ca2+ removal: aberrations in human complex I deficiency. Am. J. Physiol. Cell Physiol. 291: C308-C316 [Abstract] [Full Text]  
• Qiu, L. Y., Swarts, H. G. P., Tonk, E. C. M., Willems, P. H. G. M., Koenderink, J. B., De Pont, J. J. H. H. M. (2006). Conversion of the Low Affinity Ouabain-binding Site of Non-gastric H,K-ATPase into a High Affinity Binding Site by Substitution of Only Five Amino Acids. J. Biol. Chem. 281: 13533-13539 [Abstract] [Full Text]  
• Kenoutis, C., Efrose, R. C., Swevers, L., Lavdas, A. A., Gaitanou, M., Matsas, R., Iatrou, K. (2006). Baculovirus-mediated gene delivery into Mammalian cells does not alter their transcriptional and differentiating potential but is accompanied by early viral gene expression.. J. Virol. 80: 4135-4146 [Abstract] [Full Text]  
• Okoye, M. E., Sexton, G. L., Huang, E., McCaffery, J. M., Desai, P. (2006). Functional Analysis of the Triplex Proteins (VP19C and VP23) of Herpes Simplex Virus Type 1. J. Virol. 80: 929-940 [Abstract] [Full Text]  
• Schneeweis, L. A., Willard, D., Milla, M. E. (2005). Functional Dissection of Osteoprotegerin and Its Interaction with Receptor Activator of NF-{kappa}B Ligand. J. Biol. Chem. 280: 41155-41164 [Abstract] [Full Text]  
• Swarts, H. G. P., Koenderink, J. B., Willems, P. H. G. M., De Pont, J. J. H. H. M. (2005). The Non-gastric H,K-ATPase Is Oligomycin-sensitive and Can Function as an H+,NH4+-ATPase. J. Biol. Chem. 280: 33115-33122 [Abstract] [Full Text]  
• Settlage, S. B., See, R. G., Hanley-Bowdoin, L. (2005). Geminivirus C3 Protein: Replication Enhancement and Protein Interactions. J. Virol. 79: 9885-9895 [Abstract] [Full Text]  
• Eshaghi, M., Tan, W. S., Ong, S. T., Yusoff, K. (2005). Purification and Characterization of Nipah Virus Nucleocapsid Protein Produced in Insect Cells. J. Clin. Microbiol. 43: 3172-3177 [Abstract] [Full Text]  
• Stewart, T. M., Huijskens, I., Willis, L. G., Theilmann, D. A. (2005). The Autographa californica Multiple Nucleopolyhedrovirus ie0-ie1 Gene Complex Is Essential for Wild-Type Virus Replication, but either IE0 or IE1 Can Support Virus Growth. J. Virol. 79: 4619-4629 [Abstract] [Full Text]  
• Watson, C., Jenkinson, S., Kazmierski, W., Kenakin, T. (2005). The CCR5 Receptor-Based Mechanism of Action of 873140, a Potent Allosteric Noncompetitive HIV Entry Inhibitor. Mol. Pharmacol. 67: 1268-1282 [Abstract] [Full Text]  
• Swarts, H. G. P., Koenderink, J. B., Willems, P. H. G. M., Krieger, E., De Pont, J. J. H. H. M. (2005). Asn792 Participates in the Hydrogen Bond Network Around the K+-binding Pocket of Gastric H,K-ATPase. J. Biol. Chem. 280: 11488-11494 [Abstract] [Full Text]  
• Okano, K., Vanarsdall, A. L., Rohrmann, G. F. (2004). Characterization of a Baculovirus Lacking the Alkaline Nuclease Gene. J. Virol. 78: 10650-10656 [Abstract] [Full Text]  
• Dai, X., Stewart, T. M., Pathakamuri, J. A., Li, Q., Theilmann, D. A. (2004). Autographa californica Multiple Nucleopolyhedrovirus exon0 (orf141), Which Encodes a RING Finger Protein, Is Required for Efficient Production of Budded Virus. J. Virol. 78: 9633-9644 [Abstract] [Full Text]  
• Bubeck, A., Wagner, M., Ruzsics, Z., Lotzerich, M., Iglesias, M., Singh, I. R., Koszinowski, U. H. (2004). Comprehensive Mutational Analysis of a Herpesvirus Gene in the Viral Genome Context Reveals a Region Essential for Virus Replication. J. Virol. 78: 8026-8035 [Abstract] [Full Text]  
• Westenberg, M., Veenman, F., Roode, E. C., Goldbach, R. W., Vlak, J. M., Zuidema, D. (2004). Functional Analysis of the Putative Fusion Domain of the Baculovirus Envelope Fusion Protein F. J. Virol. 78: 6946-6954 [Abstract] [Full Text]  
• Koenderink, J. B., Swarts, H. G. P., Willems, P. H. G. M., Krieger, E., De Pont, J. J. H. H. M. (2004). A Conformation-specific Interhelical Salt Bridge in the K+ Binding Site of Gastric H,K-ATPase. J. Biol. Chem. 279: 16417-16424 [Abstract] [Full Text]  
• Castillo, A. G., Kong, L. J., Hanley-Bowdoin, L., Bejarano, E. R. (2004). Interaction between a Geminivirus Replication Protein and the Plant Sumoylation System. J. Virol. 78: 2758-2769 [Abstract] [Full Text]  
• Carreira, A., Menendez, M., Reguera, J., Almendral, J. M., Mateu, M. G. (2004). In Vitro Disassembly of a Parvovirus Capsid and Effect on Capsid Stability of Heterologous Peptide Insertions in Surface Loops. J. Biol. Chem. 279: 6517-6525 [Abstract] [Full Text]  
• McCormick, C. J., Challinor, L., Macdonald, A., Rowlands, D. J., Harris, M. (2004). Introduction of replication-competent hepatitis C virus transcripts using a tetracycline-regulable baculovirus delivery system. J. Gen. Virol. 85: 429-439 [Abstract] [Full Text]  
• Pijlman, G. P., van Schijndel, J. E., Vlak, J. M. (2003). Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expression vectors upon passage in insect cells. J. Gen. Virol. 84: 2669-2678 [Abstract] [Full Text]  
• Tijssen, P., Li, Y., El-Far, M., Szelei, J., Letarte, M., Zadori, Z. (2003). Organization and Expression Strategy of the Ambisense Genome of Densonucleosis Virus of Galleria mellonella. J. Virol. 77: 10357-10365 [Abstract] [Full Text]  
• Airenne, K. J., Peltomaa, E., Hytonen, V. P., Laitinen, O. H., Yla-Herttuala, S. (2003). Improved generation of recombinant baculovirus genomes in Escherichia coli. Nucleic Acids Res 31: e101-e101 [Abstract] [Full Text]  
• Jenkinson, S., Mc Coy, D. C., Kerner, S. A., Ferris, R. G., Lawrence, W. K., Clay, W. C., Condreay, J. P., Smith, C. D. (2003). Development of a Novel High-Throughput Surrogate Assay to Measure HIV Envelope/CCR5/CD4-Mediated Viral/Cell Fusion Using BacMam Baculovirus Technology. J Biomol Screen 8: 463-470 [Abstract]  
• Pijlman, G. P., Pruijssers, A. J. P., Vlak, J. M. (2003). Identification of pif-2, a third conserved baculovirus gene required for per os infection of insects. J. Gen. Virol. 84: 2041-2049 [Abstract] [Full Text]  
• Brini, M., Coletto, L., Pierobon, N., Kraev, N., Guerini, D., Carafoli, E. (2003). A Comparative Functional Analysis of Plasma Membrane Ca2+ Pump Isoforms in Intact Cells. J. Biol. Chem. 278: 24500-24508 [Abstract] [Full Text]  
• Zhang, S. X., Han, Y., Blissard, G. W. (2003). Palmitoylation of the Autographa californica Multicapsid Nucleopolyhedrovirus Envelope Glycoprotein GP64: Mapping, Functional Studies, and Lipid Rafts. J. Virol. 77: 6265-6273 [Abstract] [Full Text]  
• Chroni, A., Liu, T., Gorshkova, I., Kan, H.-Y., Uehara, Y., von Eckardstein, A., Zannis, V. I. (2003). The Central Helices of ApoA-I Can Promote ATP-binding Cassette Transporter A1 (ABCA1)-mediated Lipid Efflux. AMINO ACID RESIDUES 220-231 OF THE WILD-TYPE ApoA-I ARE REQUIRED FOR LIPID EFFLUX IN VITRO AND HIGH DENSITY LIPOPROTEIN FORMATION IN VIVO. J. Biol. Chem. 278: 6719-6730 [Abstract] [Full Text]  
• Zhao, Y., Chapman, D. A. G., Jones, I. M. (2003). Improving baculovirus recombination. Nucleic Acids Res 31: e6-e6 [Abstract] [Full Text]  
• Kaba, S. A., Hemmes, J. C., van Lent, J. W.M., Vlak, J. M., Nene, V., Musoke, A. J., van Oers, M. M. (2003). Baculovirus surface display of Theileria parva p67 antigen preserves the conformation of sporozoite-neutralizing epitopes. Protein Eng Des Sel 16: 73-78 [Abstract] [Full Text]  
• Lung, O. Y., Cruz-Alvarez, M., Blissard, G. W. (2002). Ac23, an Envelope Fusion Protein Homolog in the Baculovirus Autographa californica Multicapsid Nucleopolyhedrovirus, Is a Viral Pathogenicity Factor. J. Virol. 77: 328-339 [Abstract] [Full Text]  
• Lu, L., Du, Q., Chejanovsky, N. (2002). Reduced Expression of the Immediate-Early Protein IE0 Enables Efficient Replication of Autographa californica Multiple Nucleopolyhedrovirus in Poorly Permissive Spodoptera littoralis Cells. J. Virol. 77: 535-545 [Abstract] [Full Text]  
• Cook, C. S., Berry, L. M., Kim, D. H., Burton, E. G., Hribar, J. D., Zhang, L. (2002). Involvement of CYP3A in the Metabolism of Eplerenone in Humans and Dogs: Differential Metabolism by CYP3A4 and CYP3A5. Drug Metab. Dispos. 30: 1344-1351 [Abstract] [Full Text]  
• Lee, S.-J., Buhler, D. R. (2002). Functional Properties of a Rainbow Trout CYP3A27 Expressed by Recombinant Baculovirus in Insect Cells. Drug Metab. Dispos. 30: 1406-1412 [Abstract] [Full Text]  
• Domi, A., Moss, B. (2002). Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells. Proc. Natl. Acad. Sci. USA 99: 12415-12420 [Abstract] [Full Text]  
• Hahn, G., Khan, H., Baldanti, F., Koszinowski, U. H., Revello, M. G., Gerna, G. (2002). The Human Cytomegalovirus Ribonucleotide Reductase Homolog UL45 Is Dispensable for Growth in Endothelial Cells, as Determined by a BAC-Cloned Clinical Isolate of Human Cytomegalovirus with Preserved Wild-Type Characteristics. J. Virol. 76: 9551-9555 [Abstract] [Full Text]  
• Liu, T., Krieger, M., Kan, H.-Y., Zannis, V. I. (2002). The Effects of Mutations in Helices 4 and 6 of ApoA-I on Scavenger Receptor Class B Type I (SR-BI)-mediated Cholesterol Efflux Suggest That Formation of a Productive Complex between Reconstituted High Density Lipoprotein and SR-BI Is Required for Efficient Lipid Transport. J. Biol. Chem. 277: 21576-21584 [Abstract] [Full Text]  
• Bouche, N., Scharlat, A., Snedden, W., Bouchez, D., Fromm, H. (2002). A Novel Family of Calmodulin-binding Transcription Activators in Multicellular Organisms. J. Biol. Chem. 277: 21851-21861 [Abstract] [Full Text]  
• Lin, G., Blissard, G. W. (2002). Analysis of an Autographa californica Multicapsid Nucleopolyhedrovirus lef-6-Null Virus: LEF-6 Is Not Essential for Viral Replication but Appears To Accelerate Late Gene Transcription. J. Virol. 76: 5503-5514 [Abstract] [Full Text]  
• Pijlman, G. P., Dortmans, J. C. F. M., Vermeesch, A. M. G., Yang, K., Martens, D. E., Goldbach, R. W., Vlak, J. M. (2002). Pivotal Role of the Non-hr Origin of DNA Replication in the Genesis of Defective Interfering Baculoviruses. J. Virol. 76: 5605-5611 [Abstract] [Full Text]  
• Lung, O., Westenberg, M., Vlak, J. M., Zuidema, D., Blissard, G. W. (2002). Pseudotyping Autographa californica Multicapsid Nucleopolyhedrovirus (AcMNPV): F Proteins from Group II NPVs Are Functionally Analogous to AcMNPV GP64. J. Virol. 76: 5729-5736 [Abstract] [Full Text]  
• Scheffczik, H., Savva, C. G. W., Holzenburg, A., Kolesnikova, L., Bogner, E. (2002). The terminase subunits pUL56 and pUL89 of human cytomegalovirus are DNA-metabolizing proteins with toroidal structure. Nucleic Acids Res 30: 1695-1703 [Abstract] [Full Text]  
• GUERINI, D., GUIDI, F., CARAFOLI, E. (2002). Differential membrane targeting of the SERCA and PMCA calcium pumps: experiments with recombinant chimeras. FASEB J. 16: 519-528 [Abstract] [Full Text]  
• Lin, G., Blissard, G. W. (2002). Analysis of an Autographa californica Nucleopolyhedrovirus lef-11 Knockout: LEF-11 Is Essential for Viral DNA Replication. J. Virol. 76: 2770-2779 [Abstract] [Full Text]  
• Huang, Y.-W., Pineau, I., Chang, H.-J., Azzi, A., Bellemare, V., Laberge, S., Lin, S.-X. (2001). Critical Residues for the Specificity of Cofactors and Substrates in Human Estrogenic 17{beta}-Hydroxysteroid Dehydrogenase 1: Variants Designed from the Three-Dimensional Structure of the Enzyme. Mol. Endocrinol. 15: 2010-2020 [Abstract] [Full Text]  
• Dong, H., Haining, R. L., Thummel, K. E., Rettie, A. E., Nelson, S. D. (2001). Involvement of Human Cytochrome P450 2D6 in the Bioactivation of Acetaminophen. Drug Metab. Dispos. 28: 1397-1400 [Abstract] [Full Text]  
• Trippett, T. M., Garcia, S., Manova, K., Mody, R., Cohen-Gould, L., Flintoff, W., Bertino, J. R. (2001). Localization of a Human Reduced Folate Carrier Protein in the Mitochondrial as Well as the Cell Membrane of Leukemia Cells. Cancer Res. 61: 1941-1947 [Abstract] [Full Text]  
• Harro, C. D., Pang, Y.-Y. S., Roden, R. B. S., Hildesheim, A., Wang, Z., Reynolds, M. J., Mast, T. C., Robinson, R., Murphy, B. R., Karron, R. A., Dillner, J., Schiller, J. T., Lowy, D. R. (2001). Safety and Immunogenicity Trial in Adult Volunteers of a Human Papillomavirus 16 L1 Virus-Like Particle Vaccine. JNCI J Natl Cancer Inst 93: 284-292 [Abstract] [Full Text]  
• Merrihew, R. V., Clay, W. C., Condreay, J. P., Witherspoon, S. M., Dallas, W. S., Kost, T. A. (2001). Chromosomal Integration of Transduced Recombinant Baculovirus DNA in Mammalian Cells. J. Virol. 75: 903-909 [Abstract] [Full Text]  
• Hartley, J. L., Temple, G. F., Brasch, M. A. (2000). DNA Cloning Using In Vitro Site-Specific Recombination. Genome Res 10: 1788-1795 [Abstract] [Full Text]  
• Hobom, U., Brune, W., Messerle, M., Hahn, G., Koszinowski, U. H. (2000). Fast Screening Procedures for Random Transposon Libraries of Cloned Herpesvirus Genomes: Mutational Analysis of Human Cytomegalovirus Envelope Glycoprotein Genes. J. Virol. 74: 7720-7729 [Abstract] [Full Text]  
• Orozco, B. M., Kong, L.-J., Batts, L. A., Elledge, S., Hanley-Bowdoin, L. (2000). The Multifunctional Character of a Geminivirus Replication Protein Is Reflected by Its Complex Oligomerization Properties. J. Biol. Chem. 275: 6114-6122 [Abstract] [Full Text]  
• Yokoyama, N., Fujii, K., Hirata, M., Tamai, K., Kiyono, T., Kuzushima, K., Nishiyama, Y., Fujita, M., Tsurumi, T. (1999). Assembly of the Epstein-Barr virus BBLF4, BSLF1 and BBLF2/3 proteins and their interactive properties. J. Gen. Virol. 80: 2879-2887 [Abstract] [Full Text]  
• Suter, M., Lew, A. M., Grob, P., Adema, G. J., Ackermann, M., Shortman, K., Fraefel, C. (1999). BAC-VAC, a novel generation of (DNA) vaccines: A bacterial artificial chromosome (BAC) containing a replication-competent, packaging-defective virus genome induces protective immunity against herpes simplex virus 1. Proc. Natl. Acad. Sci. USA 96: 12697-12702 [Abstract] [Full Text]  
• Paulson, S. K., Engel, L., Reitz, B., Bolten, S., Burton, E. G., Maziasz, T. J., Yan, B., Schoenhard, G. L. (1999). Evidence for Polymorphism in the Canine Metabolism of the Cyclooxygenase 2 Inhibitor, Celecoxib. Drug Metab. Dispos. 27: 1133-1142 [Abstract] [Full Text]  
• Effertz, K., Hinderlich, S., Reutter, W. (1999). Selective Loss of either the Epimerase or Kinase Activity of UDP-N-acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase due to Site-directed Mutagenesis Based on Sequence Alignments. J. Biol. Chem. 274: 28771-28778 [Abstract] [Full Text]  
• Furman-Matarasso, N., Cohen, E., Du, Q., Chejanovsky, N., Hanania, U., Avni, A. (1999). A Point Mutation in the Ethylene-Inducing Xylanase Elicitor Inhibits the beta -1-4-Endoxylanase Activity But Not the Elicitation Activity. Plant Physiol. 121: 345-352 [Abstract] [Full Text]  
• Rijsewijk, F., Kaashoek, M., Langeveld, J., Meloen, R, Judek, J, Bienkowska-Szewczyk, K, Maris-Veldhuis, M., van Oirschot, J. (1999). Epitopes on glycoprotein C of bovine herpesvirus-1 (BHV-1) that allow differentiation between BHV-1.1 and BHV-1.2 strains. J. Gen. Virol. 80: 1477-1483 [Abstract]  
• Koenderink, J. B., Swarts, H. G. P., Hermsen, H. P. H., De Pont, J. J. H. H. M. (1999). The {beta}-Subunits of Na+,K+-ATPase and Gastric H+,K+-ATPase Have a High Preference for Their Own {alpha}-Subunit and Affect the K+ Affinity of These Enzymes. J. Biol. Chem. 274: 11604-11610 [Abstract] [Full Text]  
• Lanford, R. E., Kim, Y.-H., Lee, H., Notvall, L., Beames, B. (1999). Mapping of the Hepatitis B Virus Reverse Transcriptase TP and RT Domains by Transcomplementation for Nucleotide Priming and by Protein-Protein Interaction. J. Virol. 73: 1885-1893 [Abstract] [Full Text]  
• Zhou, D., Dinter, A., Gallego, R. G., Kamerling, J. P., Vliegenthart, J. F. G., Berger, E. G., Hennet, T. (1999). A beta -1,3-N-acetylglucosaminyltransferase with poly-N-acetyllactosamine synthase activity is structurally related to beta -1,3-galactosyltransferases. Proc. Natl. Acad. Sci. USA 96: 406-411 [Abstract] [Full Text]  
• Condreay, J. P., Witherspoon, S. M., Clay, W. C., Kost, T. A. (1999). Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc. Natl. Acad. Sci. USA 96: 127-132 [Abstract] [Full Text]  
• Chakravarty, L., Rogers, L., Quach, T., Breckenridge, S., Kolattukudy, P. E. (1998). Lysine 58 and Histidine 66 at the C-terminal alpha -Helix of Monocyte Chemoattractant Protein-1 Are Essential for Glycosaminoglycan Binding. J. Biol. Chem. 273: 29641-29647 [Abstract] [Full Text]  
• Sawado, T., Hirose, F., Takahashi, Y., Sasaki, T., Shinomiya, T., Sakaguchi, K., Matsukage, A., Yamaguchi, M. (1998). The DNA Replication-related Element (DRE)/DRE-binding Factor System Is a Transcriptional Regulator of the Drosophila E2F Gene. J. Biol. Chem. 273: 26042-26051 [Abstract] [Full Text]  
• Orozco, B. M., Hanley-Bowdoin, L. (1998). Conserved Sequence and Structural Motifs Contribute to the DNA Binding and Cleavage Activities of a Geminivirus Replication Protein. J. Biol. Chem. 273: 24448-24456 [Abstract] [Full Text]  
• Feng, L., Cohen, D. E. (1998). Baculovirus-mediated expression of recombinant rat phosphatidylcholine transfer protein. J. Lipid Res. 39: 1862-1869 [Abstract] [Full Text]  
• Baumert, T. F., Ito, S., Wong, D. T., Liang, T. J. (1998). Hepatitis C Virus Structural Proteins Assemble into Viruslike Particles in Insect Cells. J. Virol. 72: 3827-3836 [Abstract] [Full Text]  
• Maree, S, Durbach, S, Huismans, H (1998). Intracellular production of African horsesickness virus core-like particles by expression of the two major core proteins, VP3 and VP7, in insect cells. J. Gen. Virol. 79: 333-337 [Abstract]  
• Rossi, V., Bally, I., Thielens, N. M., Esser, A. F., Arlaud, G. J. (1998). Baculovirus-mediated Expression of Truncated Modular Fragments from the Catalytic Region of Human Complement Serine Protease C1s. EVIDENCE FOR THE INVOLVEMENT OF BOTH COMPLEMENT CONTROL PROTEIN MODULES IN THE RECOGNITION OF THE C4 PROTEIN SUBSTRATE. J. Biol. Chem. 273: 1232-1239 [Abstract] [Full Text]  
• Hennet, T., Dinter, A., Kuhnert, P., Mattu, T. S., Rudd, P. M., Berger, E. G. (1998). Genomic Cloning and Expression of Three Murine UDP-galactose: beta -N-Acetylglucosamine beta 1,3-Galactosyltransferase Genes. J. Biol. Chem. 273: 58-65 [Abstract] [Full Text]  
• Chen, W.-J., Armour, S., Way, J., Chen, G., Watson, C., Irving, P., Cobb, J., Kadwell, S., Beaumont, K., Rimele, T., Kenakin, T. (1997). Expression Cloning and Receptor Pharmacology of Human Calcitonin Receptors from MCF-7 Cells and Their Relationship to Amylin Receptors. Mol. Pharmacol. 52: 1164-1175 [Abstract] [Full Text]  
• Banik, U., Wang, G.-A., Wagner, P. D., Kaufman, S. (1997). Interaction of Phosphorylated Tryptophan Hydroxylase with 14-3-3 Proteins. J. Biol. Chem. 272: 26219-26225 [Abstract] [Full Text]  
• Martins, L. M., Kottke, T., Mesner, P. W., Basi, G. S., Sinha, S., Frigon, N. Jr., Tatar, E., Tung, J. S., Bryant, K., Takahashi, A., Svingen, P. A., Madden, B. J., McCormick, D. J., Earnshaw, W. C., Kaufmann, S. H. (1997). Activation of Multiple Interleukin-1beta Converting Enzyme Homologues in Cytosol and Nuclei of HL-60 Cells during Etoposide-induced Apoptosis. J. Biol. Chem. 272: 7421-7430 [Abstract] [Full Text]  
• Wolf, M. J., Gross, R. W. (1996). Expression, Purification, and Kinetic Characterization of a Recombinant 80-kDa Intracellular Calcium-independent Phospholipase A2. J. Biol. Chem. 271: 30879-30885 [Abstract] [Full Text]  
• Stanley, S. L. Jr., Tian, K., Koester, J. P., Li, E. (1995). The Serine-rich Entamoeba histolytica Protein Is a Phosphorylated Membrane Protein Containing O-Linked Terminal N-Acetylglucosamine Residues. J. Biol. Chem. 270: 4121-4126 [Abstract] [Full Text]  
• Schild, D., Lio, Y.-c., Collins, D. W., Tsomondo, T., Chen, D. J. (2000). Evidence for Simultaneous Protein Interactions between Human Rad51 Paralogs. J. Biol. Chem. 275: 16443-16449 [Abstract] [Full Text]  
• Liadaki, K. N., Liu, T., Xu, S., Ishida, B. Y., Duchateaux, P. N., Krieger, J. P., Kane, J., Krieger, M., Zannis, V. I. (2000). Binding of High Density Lipoprotein (HDL) and Discoidal Reconstituted HDL to the HDL Receptor Scavenger Receptor Class B Type I. EFFECT OF LIPID ASSOCIATION AND APOA-I MUTATIONS ON RECEPTOR BINDING. J. Biol. Chem. 275: 21262-21271 [Abstract] [Full Text]  
• Guerini, D., Zecca-Mazza, A., Carafoli, E. (2000). Single Amino Acid Mutations in Transmembrane Domain 5 Confer to the Plasma Membrane Ca2+ Pump Properties Typical of the Ca2+ Pump of Endo(sarco)plasmic Reticulum. J. Biol. Chem. 275: 31361-31368 [Abstract] [Full Text]  
• Koenderink, J. B., Swarts, H. G. P., Stronks, H. C., Hermsen, H. P. H., Willems, P. H. G. M., De Pont, J. J. H. H. M. (2001). Chimeras of X+,K+-ATPases. THE M1-M6 REGION OF Na+,K+-ATPase IS REQUIRED FOR Na+-ACTIVATED ATPase ACTIVITY, WHEREAS THE M7-M10 REGION OF H+,K+-ATPase IS INVOLVED IN K+ DE-OCCLUSION. J. Biol. Chem. 276: 11705-11711 [Abstract] [Full Text]  
• Swarts, H. G. P., Koenderink, J. B., Hermsen, H. P. H., Willems, P. H. G. M., De Pont, J. J. H. H. M. (2001). K+-independent Gastric H+,K+-ATPase Activity. DISSOCIATION OF K+-INDEPENDENT DEPHOSPHORYLATION AND PREFERENCE FOR THE E1 CONFORMATION BY COMBINED MUTAGENESIS OF TRANSMEMBRANE GLUTAMATE RESIDUES. J. Biol. Chem. 276: 36909-36916 [Abstract] [Full Text]  
• Brignola, P. S., Lackey, K., Kadwell, S. H., Hoffman, C., Horne, E., Carter, H. L., Stuart, J. D., Blackburn, K., Moyer, M. B., Alligood, K. J., Knight, W. B., Wood, E. R. (2002). Comparison of the Biochemical and Kinetic Properties of the Type 1 Receptor Tyrosine Kinase Intracellular Domains. DEMONSTRATION OF DIFFERENTIAL SENSITIVITY TO KINASE INHIBITORS. J. Biol. Chem. 277: 1576-1585 [Abstract] [Full Text]  
• Tsivkovskii, R., Eisses, J. F., Kaplan, J. H., Lutsenko, S. (2002). Functional Properties of the Copper-transporting ATPase ATP7B (The Wilson's Disease Protein) Expressed in Insect Cells. J. Biol. Chem. 277: 976-983 [Abstract] [Full Text]