This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Asjö, B Articles by Autran, B Search for Related Content PubMed PubMed Citation Articles by Asjö, B Articles by Autran, B

 Previous Article  |  Next Article 

J Virol. 1993 July; 67(7): 4395-4398

A novel mode of human immunodeficiency virus type 1 (HIV-1) activation: ligation of CD28 alone induces HIV-1 replication in naturally infected lymphocytes.

Laboratoire d'Immunologie Cellulaire et Tissulaire, Hôpital Pitié Salpetrière, Paris, France.

ABSTRACT

Induction of human immunodeficiency virus (HIV) replication in infected CD4+ T lymphocytes requires cellular activation. The ligation of CD28, a signal-transducing receptor with a natural ligand on activated B cells and antigen-presenting cells, provides a costimulating signal for interleukin 2 production and T-cell proliferation as well as coactivation of the transfected HIV long terminal repeat in Jurkat cells. The aim of the present study was to investigate the ability of CD28 ligation to activate HIV type 1 (HIV-1) replication in naturally infected CD4+ lymphocytes either alone or in combination with immobilized anti-CD3 monoclonal antibody. Our results show that HIV-1 was successfully isolated from 16 of 28 patients. For 5 of these 16, virus was isolated only when anti-CD28 was added in combination with the anti-CD3. Moreover, stimulation by anti-CD28 alone induced HIV-1 replication in 5 of 12 patients tested, in the absence of cell proliferation. We found no correlation between the level of CD3- or CD28-induced proliferative response and induction of HIV-1 replication. Therefore, CD28 ligation, a nonmitogenic CD4+ T-cell activation signal, is sufficient to induce transcription and replication of HIV-1 in naturally infected lymphocytes.

This article has been cited by other articles:

• Holm, G. H., Gabuzda, D. (2005). Distinct Mechanisms of CD4+ and CD8+ T-Cell Activation and Bystander Apoptosis Induced by Human Immunodeficiency Virus Type 1 Virions. J. Virol. 79: 6299-6311 [Abstract] [Full Text]  
• Barat, C., Tremblay, M. J. (2002). Engagement of CD43 Enhances Human Immunodeficiency Virus Type 1 Transcriptional Activity and Virus Production That Is Induced upon TCR/CD3 Stimulation. J. Biol. Chem. 277: 28714-28724 [Abstract] [Full Text]  
• Li, Q., Furman, S. A., Bradford, C. R., Chang, A. E. (1999). Expanded Tumor-reactive CD4+ T-Cell Responses to Human Cancers Induced by Secondary Anti-CD3/Anti-CD28 Activation. Clin. Cancer Res. 5: 461-469 [Abstract] [Full Text]  
• Cole, S. W., Korin, Y. D., Fahey, J. L., Zack, J. A. (1998). Norepinephrine Accelerates HIV Replication Via Protein Kinase A-Dependent Effects on Cytokine Production. J. Immunol. 161: 610-616 [Abstract] [Full Text]  
• Haffar, O. K., Smithgall, M. D., Popov, S., Ulrich, P., Bruce, A. G., Nadler, S. G., Cerami, A., Bukrinsky, M. I. (1998). CNI-H0294, a Nuclear Importation Inhibitor of the Human Immunodeficiency Virus Type 1 Genome, Abrogates Virus Replication in Infected Activated Peripheral Blood Mononuclear Cells. Antimicrob. Agents Chemother. 42: 1133-1138 [Abstract] [Full Text]  
• Kimata, J. T., Mozaffarian, A., Overbaugh, J. (1998). A Lymph Node-Derived Cytopathic Simian Immunodeficiency Virus Mne Variant Replicates in Nonstimulated Peripheral Blood Mononuclear Cells. J. Virol. 72: 245-256 [Abstract] [Full Text]