This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Karlen, S Articles by Beard, P Search for Related Content PubMed PubMed Citation Articles by Karlen, S Articles by Beard, P

 Previous Article  |  Next Article 

J Virol. 1993 July; 67(7): 4296-4306

Identification and characterization of novel promoters in the genome of human papillomavirus type 18.

Department of Virology, Swiss Institute for Experimental Cancer Research, Epalinges.

ABSTRACT

Most studies on the regulation of gene expression in human papillomaviruses (HPV) have focused on the promoter for the early genes E6 and E7. This promoter is located at the junction between the long control region and the E6 open reading frame. RNA mapping studies have suggested that additional promoters may exist in other parts of the genome. In this study, we used a combination of transcription in vitro and an analysis of RNA produced in vivo in transfected cells to identify three novel promoters in the genome of human papillomavirus type 18. These promoters are located in front of the E2 gene (P2598), within the E2 coding sequences (P3036), and at the end of the L2 open reading frame (P5600). They were active in HeLa cells, as shown by a chloramphenicol acetyltransferase assay. The activity of the P3036 promoter was stimulated by the bovine papillomavirus type 1 E2 protein.

This article has been cited by other articles:

• Baars, S., Bachmann, A., Levitzki, A., Rosl, F. (2003). Tyrphostin AG 555 Inhibits Bovine Papillomavirus Transcription by Changing the Ratio between E2 Transactivator/Repressor Function. J. Biol. Chem. 278: 37306-37313 [Abstract] [Full Text]  
• Schwenger, G. T. F., Kok, C. C., Arthaningtyas, E., Thomas, M. A., Sanderson, C. J., Mordvinov, V. A. (2002). Specific Activation of Human Interleukin-5 Depends on de Novo Synthesis of an AP-1 Complex. J. Biol. Chem. 277: 47022-47027 [Abstract] [Full Text]  
• Schwenger, G. T. F., Fournier, R., Kok, C. C., Mordvinov, V. A., Yeoman, D., Sanderson, C. J. (2001). GATA-3 Has Dual Regulatory Functions in Human Interleukin-5 Transcription. J. Biol. Chem. 276: 48502-48509 [Abstract] [Full Text]  
• Salerno, M. S., Mordvinov, V. A., Sanderson, C. J. (2000). Binding of Octamer Factors to a Novel 3'-Positive Regulatory Element in the Mouse Interleukin-5 Gene. J. Biol. Chem. 275: 4525-4531 [Abstract] [Full Text]  
• Stubenrauch, F., Hummel, M., Iftner, T., Laimins, L. A. (2000). The E8 ˆE2C Protein, a Negative Regulator of Viral Transcription and Replication, Is Required for Extrachromosomal Maintenance of Human Papillomavirus Type 31 in Keratinocytes. J. Virol. 74: 1178-1186 [Abstract] [Full Text]  
• Braunstein, T. H., Madsen, B. S., Gavnholt, B., Rosenstierne, M. W., Koefoed Johnsen, C., Norrild, B. (1999). Identification of a new promoter in the early region of the human papillomavirus type 16 genome. J. Gen. Virol. 80: 3241-3250 [Abstract] [Full Text]  
• Remm, M., Remm, A., Ustav, M. (1999). Human Papillomavirus Type 18 E1 Protein Is Translated from Polycistronic mRNA by a Discontinuous Scanning Mechanism. J. Virol. 73: 3062-3070 [Abstract] [Full Text]