This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Melnikova, I N
Right arrow Articles by Speck, N A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Melnikova, I N
Right arrow Articles by Speck, N A

 Previous Article  |  Next Article 

J Virol. 1993 April; 67(4): 2408-2411

Sequence specificity of the core-binding factor.

I N Melnikova, B E Crute, S Wang and N A Speck

Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755.

ABSTRACT

The core-binding factor (CBF) binds the conserved core motif in mammalian type C retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts span 9 bp centered around the consensus core site. To examine the sequence preferences for CBF binding, we employed the technique of selected and amplified binding sequence footprinting (T. K. Blackwell and H. Weintraub, Science 250:1104-1110, 1990). The consensus binding site for CBF defined by selected and amplified binding sequence footprinting is PyGPyG GTPy.

J Virol. 1993 April; 67(4): 2408-2411




This article has been cited by other articles:

  • Jonason, J.H., Xiao, G., Zhang, M., Xing, L., Chen, D. (2009). Post-translational Regulation of Runx2 in Bone and Cartilage. JDR 88: 693-703 [Abstract] [Full Text]  
  • Okumura, A. J., Peterson, L. F., Okumura, F., Boyapati, A., Zhang, D.-E. (2008). t(8;21)(q22;q22) fusion proteins preferentially bind to duplicated AML1/RUNX1 DNA-binding sequences to differentially regulate gene expression. Blood 112: 1392-1401 [Abstract] [Full Text]  
  • Mertz, J. A., Kobayashi, R., Dudley, J. P. (2007). ALY Is a Common Coactivator of RUNX1 and c-Myb on the Type B Leukemogenic Virus Enhancer. J. Virol. 81: 3503-3513 [Abstract] [Full Text]  
  • Carabana, J., Ortigoza, E., Krangel, M. S. (2005). Regulation of the Murine D{delta}2 Promoter by Upstream Stimulatory Factor 1, Runx1, and c-Myb. J. Immunol. 174: 4144-4152 [Abstract] [Full Text]  
  • Sorensen, K. D., Quintanilla-Martinez, L., Kunder, S., Schmidt, J., Pedersen, F. S. (2004). Mutation of All Runx (AML1/Core) Sites in the Enhancer of T-Lymphomagenic SL3-3 Murine Leukemia Virus Unmasks a Significant Potential for Myeloid Leukemia Induction and Favors Enhancer Evolution toward Induction of Other Disease Patterns. J. Virol. 78: 13216-13231 [Abstract] [Full Text]  
  • McCarthy, K. M., McDevit, D., Andreucci, A., Reeves, R., Nikolajczyk, B. S. (2003). HMGA1 Co-activates Transcription in B Cells through Indirect Association with DNA. J. Biol. Chem. 278: 42106-42114 [Abstract] [Full Text]  
  • Stock, M., Schafer, H., Stricker, S., Gross, G., Mundlos, S., Otto, F. (2003). Expression of Galectin-3 in Skeletal Tissues Is Controlled by Runx2. J. Biol. Chem. 278: 17360-17367 [Abstract] [Full Text]  
  • Mertz, J. A., Mustafa, F., Meyers, S., Dudley, J. P. (2001). Type B Leukemogenic Virus Has a T-Cell-Specific Enhancer That Binds AML-1. J. Virol. 75: 2174-2184 [Abstract] [Full Text]  
  • Lee, K.-S., Kim, H.-J., Li, Q.-L., Chi, X.-Z., Ueta, C., Komori, T., Wozney, J. M., Kim, E.-G., Choi, J.-Y., Ryoo, H.-M., Bae, S.-C. (2000). Runx2 Is a Common Target of Transforming Growth Factor beta 1 and Bone Morphogenetic Protein 2, and Cooperation between Runx2 and Smad5 Induces Osteoblast-Specific Gene Expression in the Pluripotent Mesenchymal Precursor Cell Line C2C12. Mol. Cell. Biol. 20: 8783-8792 [Abstract] [Full Text]  
  • Martiney, M. J., Rulli, K., Beaty, R., Levy, L. S., Lenz, J. (1999). Selection of Reversions and Suppressors of a Mutation in the CBF Binding Site of a Lymphomagenic Retrovirus. J. Virol. 73: 7599-7606 [Abstract] [Full Text]  
  • Lewis, A. F., Stacy, T., Green, W. R., Taddesse-Heath, L., Hartley, J. W., Speck, N. A. (1999). Core-Binding Factor Influences the Disease Specificity of Moloney Murine Leukemia Virus. J. Virol. 73: 5535-5547 [Abstract] [Full Text]  
  • Martiney, M. J., Levy, L. S., Lenz, J. (1999). Suppressor Mutations within the Core Binding Factor (CBF/AML1) Binding Site of a T-Cell Lymphomagenic Retrovirus. J. Virol. 73: 2143-2152 [Abstract] [Full Text]  
  • Kramer, S., Jinks, T., Schedl, P, Gergen, J. (1999). Direct activation of Sex-lethal transcription by the Drosophila runt protein. Development 126: 191-200 [Abstract]  
  • Erman, B., Cortes, M., Nikolajczyk, B. S., Speck, N. A., Sen, R. (1998). ETS-Core Binding Factor: a Common Composite Motif in Antigen Receptor Gene Enhancers. Mol. Cell. Biol. 18: 1322-1330 [Abstract] [Full Text]  
  • Huang, X., Crute, B. E., Sun, C., Tang, Y.-Y., Kelley III, J. J., Lewis, A. F., Hartman, K. L., Laue, T. M., Speck, N. A., Bushweller, J. H. (1998). Overexpression, Purification, and Biophysical Characterization of the Heterodimerization Domain of the Core-binding Factor beta  Subunit. J. Biol. Chem. 273: 2480-2487 [Abstract] [Full Text]  
  • Tsai, C, Gergen, P (1995). Pair-rule expression of the Drosophila fushi tarazu gene: a nuclear receptor response element mediates the opposing regulatory effects of runt and hairy. Development 121: 453-462 [Abstract]  
  • Tsai, C, Gergen, J. (1994). Gap gene properties of the pair-rule gene runt during Drosophila segmentation. Development 120: 1671-1683 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»