This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dollard, S C Articles by Chow, L T Search for Related Content PubMed PubMed Citation Articles by Dollard, S C Articles by Chow, L T

 Previous Article  |  Next Article 

J Virol. 1993 March; 67(3): 1721-1726

Regulation of the human papillomavirus type 11 E6 promoter by viral and host transcription factors in primary human keratinocytes.

Department of Biochemistry, University of Rochester School of Medicine and Dentistry, New York 14642.

ABSTRACT

Human papillomavirus (HPV) type 11 is strictly trophic for epithelial cells and induces benign condylomata of the external genitalia and also causes laryngeal papillomas. Primary keratinocytes are the appropriate hosts for studies of HPV gene regulation, but they are not frequently used, owing to difficulties in culturing and low transfection efficiencies. By modifying a Polybrene transfection procedure, we achieved consistently high transfection efficiencies in primary human foreskin keratinocytes and characterized the HPV type 11 enhancer in the context of the homologous E6 promoter. Contrary to previous studies with immortalized human cervical carcinoma C-33A cells, constitutive enhancer element II in the upstream regulatory region conferred no enhancer activity and did not abrogate repression by the homologous E2 protein. Rather, repression was strong, ranging from 5.6- to 20-fold for the various enhancer deletion mutations. By deletion analysis, a strong enhancer that included three nuclear factor 1 sites and one nuclear factor 1-associated factor-binding site was localized to a 45-bp region within constitutive enhancer element I, and it showed some degree of tissue specificity.

This article has been cited by other articles:

• Baldwin, A., Pirisi, L., Creek, K. E. (2004). NFI-Ski Interactions Mediate Transforming Growth Factor {beta} Modulation of Human Papillomavirus Type 16 Early Gene Expression. J. Virol. 78: 3953-3964 [Abstract] [Full Text]  
• Sen, E., Alam, S., Meyers, C. (2004). Genetic and Biochemical Analysis of cis Regulatory Elements within the Keratinocyte Enhancer Region of the Human Papillomavirus Type 31 Upstream Regulatory Region during Different Stages of the Viral Life Cycle. J. Virol. 78: 612-629 [Abstract] [Full Text]  
• Sen, E., Bromberg-White, J. L., Meyers, C. (2002). Genetic Analysis of cis Regulatory Elements within the 5' Region of the Human Papillomavirus Type 31 Upstream Regulatory Region during Different Stages of the Viral Life Cycle. J. Virol. 76: 4798-4809 [Abstract] [Full Text]  
• Stubenrauch, F., Zobel, T., Iftner, T. (2001). The E8 Domain Confers a Novel Long-Distance Transcriptional Repression Activity on the E8^E2C Protein of High-Risk Human Papillomavirus Type 31. J. Virol. 75: 4139-4149 [Abstract] [Full Text]  
• Stünkel, W., Huang, Z., Tan, S.-H., O'Connor, M. J., Bernard, H.-U. (2000). Nuclear Matrix Attachment Regions of Human Papillomavirus Type 16 Repress or Activate the E6 Promoter, Depending on the Physical State of the Viral DNA. J. Virol. 74: 2489-2501 [Abstract] [Full Text]  
• Liu, J.-S., Kuo, S.-R., McHugh, M. M., Beerman, T. A., Melendy, T. (2000). Adozelesin Triggers DNA Damage Response Pathways and Arrests SV40 DNA Replication through Replication Protein A Inactivation. J. Biol. Chem. 275: 1391-1397 [Abstract] [Full Text]  
• O'Connor, M. J., Stünkel, W., Koh, C.-H., Zimmermann, H., Bernard, H.-U. (2000). The Differentiation-Specific Factor CDP/Cut Represses Transcription and Replication of Human Papillomaviruses through a Conserved Silencing Element. J. Virol. 74: 401-410 [Abstract] [Full Text]  
• Hou, S. Y., Wu, S.-Y., Zhou, T., Thomas, M. C., Chiang, C.-M. (2000). Alleviation of Human Papillomavirus E2-Mediated Transcriptional Repression via Formation of a TATA Binding Protein (or TFIID)-TFIIB-RNA Polymerase II-TFIIF Preinitiation Complex. Mol. Cell. Biol. 20: 113-125 [Abstract] [Full Text]  
• Vance, K. W., Campo, M. S., Morgan, I. M. (1999). An Enhanced Epithelial Response of a Papillomavirus Promoter to Transcriptional Activators. J. Biol. Chem. 274: 27839-27844 [Abstract] [Full Text]  
• Gadi, V. K., Zou, N., Liu, J.-S., Cheng, S., Broker, T. R., Sorscher, E. J., Chow, L. T. (1999). Components of Human Papillomavirus That Activate Transcription and Support Plasmid Replication in Human Airway Cells. Am. J. Respir. Cell Mol. Bio. 20: 1001-1006 [Abstract] [Full Text]  
• Stünkel, W., Bernard, H.-U. (1999). The Chromatin Structure of the Long Control Region of Human Papillomavirus Type 16 Represses Viral Oncoprotein Expression. J. Virol. 73: 1918-1930 [Abstract] [Full Text]  
• Andersen, B., Hariri, A., Pittelkow, M. R., Rosenfeld, M. G. (1997). Characterization of Skn-1a/i POU Domain Factors and Linkage to Papillomavirus Gene Expression. J. Biol. Chem. 272: 15905-15913 [Abstract] [Full Text]  
• Cheng, S, Schmidt-Grimminger, D C, Murant, T, Broker, T R, Chow, L T (1995). Differentiation-dependent up-regulation of the human papillomavirus E7 gene reactivates cellular DNA replication in suprabasal differentiated keratinocytes.. Genes Dev. 9: 2335-2349 [Abstract]