This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mosser, A G Articles by Rueckert, R R Search for Related Content PubMed PubMed Citation Articles by Mosser, A G Articles by Rueckert, R R

WIN 51711-dependent mutants of poliovirus type 3: evidence that virions decay after release from cells unless drug is present.

Institute for Molecular Virology, University of Wisconsin, Madison 53706-1596.

ABSTRACT

Twenty-two spontaneous mutants of the Sabin strain of poliovirus type 3 were selected for drug resistance by plating on HeLa cell monolayers in the presence of WIN 51711, an uncoating inhibitor. When replated in the presence and absence of drug, two classes of mutants were observed; mutants displayed either a drug-dependent or a non-drug-dependent phenotype, in the proportion 14:8. Non-drug-dependent mutants plaqued with equal efficiency in the presence or absence of drug. By contrast, drug-dependent mutants made no plaques in the absence of drug, except for revertants. In single-step growth curve experiments, however, drug-dependent mutants grew as well in the absence of drug as in its presence. This paradoxical behavior of dependent mutants was traced to extreme thermolability at 37 degrees C (12- to 30-s half-life) in the absence of drug. Thermolability was exhibited only after the virus was released from the cell, implying the presence of a cell-associated protective factor, possibly pocket factor. Thus, in the absence of a thermostabilizing drug, drug-dependent mutants decayed too rapidly after release to permit spread in the plaque assay. The thermodecay product was shown to consist of 135S particles lacking VP4.

This article has been cited by other articles:

• Arnold, J. J., Vignuzzi, M., Stone, J. K., Andino, R., Cameron, C. E. (2005). Remote Site Control of an Active Site Fidelity Checkpoint in a Viral RNA-dependent RNA Polymerase. J. Biol. Chem. 280: 25706-25716 [Abstract] [Full Text]  
• Zhang, Y., Simpson, A. A., Ledford, R. M., Bator, C. M., Chakravarty, S., Skochko, G. A., Demenczuk, T. M., Watanyar, A., Pevear, D. C., Rossmann, M. G. (2004). Structural and Virological Studies of the Stages of Virus Replication That Are Affected by Antirhinovirus Compounds. J. Virol. 78: 11061-11069 [Abstract] [Full Text]  
• Salvati, A. L., De Dominicis, A., Tait, S., Canitano, A., Lahm, A., Fiore, L. (2004). Mechanism of Action at the Molecular Level of the Antiviral Drug 3(2H)-Isoflavene against Type 2 Poliovirus. Antimicrob. Agents Chemother. 48: 2233-2243 [Abstract] [Full Text]  
• Ledford, R. M., Patel, N. R., Demenczuk, T. M., Watanyar, A., Herbertz, T., Collett, M. S., Pevear, D. C. (2004). VP1 Sequencing of All Human Rhinovirus Serotypes: Insights into Genus Phylogeny and Susceptibility to Antiviral Capsid-Binding Compounds. J. Virol. 78: 3663-3674 [Abstract] [Full Text]  
• Mateo, R., Diaz, A., Baranowski, E., Mateu, M. G. (2003). Complete Alanine Scanning of Intersubunit Interfaces in a Foot-and-Mouth Disease Virus Capsid Reveals Critical Contributions of Many Side Chains to Particle Stability and Viral Function. J. Biol. Chem. 278: 41019-41027 [Abstract] [Full Text]  
• Lee, W.-M., Wang, W. (2003). Human Rhinovirus Type 16: Mutant V1210A Requires Capsid-Binding Drug for Assembly of Pentamers To Form Virions during Morphogenesis. J. Virol. 77: 6235-6244 [Abstract] [Full Text]  
• Tsang, S. K., McDermott, B. M., Racaniello, V. R., Hogle, J. M. (2001). Kinetic Analysis of the Effect of Poliovirus Receptor on Viral Uncoating: the Receptor as a Catalyst. J. Virol. 75: 4984-4989 [Abstract] [Full Text]  
• Shimizu, H., Agoh, M., Agoh, Y., Yoshida, H., Yoshii, K., Yoneyama, T., Hagiwara, A., Miyamura, T. (2000). Mutations in the 2C Region of Poliovirus Responsible for Altered Sensitivity to Benzimidazole Derivatives. J. Virol. 74: 4146-4154 [Abstract] [Full Text]  
• Dove, A. W., Racaniello, V. R. (2000). An Antiviral Compound That Blocks Structural Transitions of Poliovirus Prevents Receptor Binding at Low Temperatures. J. Virol. 74: 3929-3931 [Abstract] [Full Text]  
• Belnap, D. M., Filman, D. J., Trus, B. L., Cheng, N., Booy, F. P., Conway, J. F., Curry, S., Hiremath, C. N., Tsang, S. K., Steven, A. C., Hogle, J. M. (2000). Molecular Tectonic Model of Virus Structural Transitions: the Putative Cell Entry States of Poliovirus. J. Virol. 74: 1342-1354 [Abstract] [Full Text]  
• Genovese, D, Catone, S, Farah, M., Gambacorta, A, Fiore, L (1999). Isolation and biological characterization of 3(2H)-isoflavene-resistant and -dependent poliovirus type 2 Sabin mutants. J. Gen. Virol. 80: 157-167 [Abstract]  
• Lewis, J. K., Bothner, B., Smith, T. J., Siuzdak, G. (1998). Antiviral agent blocks breathing of the common cold virus. Proc. Natl. Acad. Sci. USA 95: 6774-6778 [Abstract] [Full Text]  
• Arts, E. J., Quinones-Mateu, M. E., Albright, J. L., Marois, J.-P., Hough, C., Gu, Z., Wainberg, M. A. (1998). 3'-Azido-3'-Deoxythymidine (AZT) Mediates Cross-Resistance to Nucleoside Analogs in the Case of AZT-Resistant Human Immunodeficiency Virus Type 1 Variants. J. Virol. 72: 4858-4865 [Abstract] [Full Text]  
• Wang, W., Lee, W.-M., Mosser, A. G., Rueckert, R. R. (1998). WIN 52035-Dependent Human Rhinovirus 16: Assembly Deficiency Caused by Mutations near the Canyon Surface. J. Virol. 72: 1210-1218 [Abstract] [Full Text]