This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shikova, E Articles by Wong, P K Search for Related Content PubMed PubMed Citation Articles by Shikova, E Articles by Wong, P K

Next Article 

J Virol. 1993 March; 67(3): 1137-1147

Correlation of specific virus-astrocyte interactions and cytopathic effects induced by ts1, a neurovirulent mutant of Moloney murine leukemia virus.

University of Texas M. D. Anderson Cancer Center, Smithville 78957.

ABSTRACT

ts1 is a highly neuropathogenic and lymphocytopathic mutant of Moloney murine leukemia virus TB (MoMuLV-TB). We previously reported that the primary neuropathogenic determinant of ts1 maps to a single amino acid substitution, Val-25-->Ile, in precursor envelope protein gPr80env. This Val-25-->Ile substitution apparently renders gPr80env inefficient for transport from the endoplasmic reticulum to the Golgi apparatus. These findings suggest that the cytopathic effect of ts1 in neural cells might be due to the accumulation of gPr80env in the endoplasmic reticulum. Since endothelial and glial cells are targets of ts1 infection in the central nervous system, we established primary endothelial and astrocyte cultures to investigate the mechanism of cell killing caused by ts1. A continuous cell line, TB, was used as a control. Our results showed that both ts1 and MoMuLV-TB replicated and induced a cytopathic effect in astrocyte cultures, albeit to different degrees; ts1 appeared to be more lethal than MoMuLV-TB. On the other hand, ts1 and MoMuLV-TB infections of endothelial or TB cells were not cytopathic. The cytopathic effect in infected astrocytes correlated with the inefficiency of gPr80env transport and the intracellular accumulation of gPr80env as well as aberrant virus particles.

This article has been cited by other articles:

• Yoshimura, F. K., Luo, X., Zhao, X., Gerard, H. C., Hudson, A. P. (2008). Up-regulation of a cellular protein at the translational level by a retrovirus. Proc. Natl. Acad. Sci. USA 105: 5543-5548 [Abstract] [Full Text]  
• Zhao, X., Yoshimura, F. K. (2008). Expression of Murine Leukemia Virus Envelope Protein Is Sufficient for the Induction of Apoptosis. J. Virol. 82: 2586-2589 [Abstract] [Full Text]  
• Yoshimura, F. K., Luo, X. (2007). Induction of Endoplasmic Reticulum Stress in Thymic Lymphocytes by the Envelope Precursor Polyprotein of a Murine Leukemia Virus during the Preleukemic Period. J. Virol. 81: 4374-4377 [Abstract] [Full Text]  
• Jiang, Y., Scofield, V. L., Yan, M., Qiang, W., Liu, N., Reid, A. J., Lynn, W. S., Wong, P. K. Y. (2006). Retrovirus-Induced Oxidative Stress with Neuroimmunodegeneration Is Suppressed by Antioxidant Treatment with a Refined Monosodium {alpha}-Luminol (Galavit). J. Virol. 80: 4557-4569 [Abstract] [Full Text]  
• Qiang, W., Kuang, X., Liu, J., Liu, N., Scofield, V. L., Reid, A. J., Jiang, Y., Stoica, G., Lynn, W. S., Wong, P. K. Y. (2006). Astrocytes Survive Chronic Infection and Cytopathic Effects of the ts1 Mutant of the Retrovirus Moloney Murine Leukemia Virus by Upregulation of Antioxidant Defenses.. J. Virol. 80: 3273-3284 [Abstract] [Full Text]  
• Qiang, W., Cahill, J. M., Liu, J., Kuang, X., Liu, N., Scofield, V. L., Voorhees, J. R., Reid, A. J., Yan, M., Lynn, W. S., Wong, P. K. Y. (2004). Activation of Transcription Factor Nrf-2 and Its Downstream Targets in Response to Moloney Murine Leukemia Virus ts1-Induced Thiol Depletion and Oxidative Stress in Astrocytes. J. Virol. 78: 11926-11938 [Abstract] [Full Text]  
• Nanua, S., Yoshimura, F. K. (2004). Mink Epithelial Cell Killing by Pathogenic Murine Leukemia Viruses Involves Endoplasmic Reticulum Stress. J. Virol. 78: 12071-12074 [Abstract] [Full Text]  
• Dimcheff, D. E., Faasse, M. A., McAtee, F. J., Portis, J. L. (2004). Endoplasmic Reticulum (ER) Stress Induced by a Neurovirulent Mouse Retrovirus Is Associated with Prolonged BiP Binding and Retention of a Viral Protein in the ER. J. Biol. Chem. 279: 33782-33790 [Abstract] [Full Text]  
• Murphy, S. L., Honczarenko, M. J., Dugger, N. V., Hoffman, P. M., Gaulton, G. N. (2004). Disparate Regions of Envelope Protein Regulate Syncytium Formation versus Spongiform Encephalopathy in Neurological Disease Induced by Murine Leukemia Virus TR. J. Virol. 78: 8392-8399 [Abstract] [Full Text]  
• Nanua, S., Yoshimura, F. K. (2004). Differential Cell Killing by Lymphomagenic Murine Leukemia Viruses Occurs Independently of p53 Activation and Mitochondrial Damage. J. Virol. 78: 5088-5096 [Abstract] [Full Text]  
• Dimcheff, D. E., Askovic, S., Baker, A. H., Johnson-Fowler, C., Portis, J. L. (2003). Endoplasmic Reticulum Stress Is a Determinant of Retrovirus-Induced Spongiform Neurodegeneration. J. Virol. 77: 12617-12629 [Abstract] [Full Text]  
• Zhang, M., Thurig, S., Tsirigotis, M., Wong, P. K. Y., Reuhl, K. R., Gray, D. A. (2003). Effects of Mutant Ubiquitin on ts1 Retrovirus-Mediated Neuropathology. J. Virol. 77: 7193-7201 [Abstract] [Full Text]  
• Hansen, R., Czub, S., Werder, E., Herold, J., Gosztonyi, G., Gelderblom, H., Schimmer, S., Mazgareanu, S., ter Meulen, V., Czub, M. (2000). Abundant Defective Viral Particles Budding from Microglia in the Course of Retroviral Spongiform Encephalopathy. J. Virol. 74: 1775-1780 [Abstract] [Full Text]  
• Lynch, W. P., Sharpe, A. H. (2000). Differential Glycosylation of the Cas-Br-E Env Protein Is Associated with Retrovirus-Induced Spongiform Neurodegeneration. J. Virol. 74: 1558-1565 [Abstract] [Full Text]