This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, J C Articles by Chao, Y C Search for Related Content PubMed PubMed Citation Articles by Lee, J C Articles by Chao, Y C

 Previous Article  |  Next Article 

J Virol. 1993 December; 67(12): 6989-6994

Superinfection-induced apoptosis and its correlation with the reduction of viral progeny in cells persistently infected with Hz-1 baculovirus.

Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China.

ABSTRACT

Differential induction of necrosis or apoptosis was found upon challenge of cells of the insect Spodoptera frugiperda productively or persistently infected with Hz-1 baculovirus, respectively. Unlike parental SF9 cells, which were essentially all killed by virally induced necrosis, persistently infected cells underwent a process of massive cell death by apoptosis; cells which were not killed by apoptosis then reestablished a cell monolayer. Upon viral challenge, the yield of viral progeny was reduced greatly in persistently virus-infected cells but not in parental cells. Immunolabelling of individual cells revealed that upon viral challenge, production of viral progeny was detectable only in necrotic cells and not in apoptotic cells. These results indicated that induction of apoptosis greatly reduces the yield of viral progeny in cells persistently infected with Hz-1 baculovirus. This is the first report of apoptosis induction in persistently infected cells upon viral superinfection.

This article has been cited by other articles:

• Lee, D.-F., Chen, C.-C., Hsu, T.-A., Juang, J.-L. (2000). A Baculovirus Superinfection System: Efficient Vehicle for Gene Transfer into Drosophila S2 Cells. J. Virol. 74: 11873-11880 [Abstract] [Full Text]  
• Geng, Y., Shane, R. B., Berencsi, K., Gonczol, E., Zaki, M. H., Margolis, D. J., Trinchieri, G., Rook, A. H. (2000). Chlamydia pneumoniae Inhibits Apoptosis in Human Peripheral Blood Mononuclear Cells Through Induction of IL-10. J. Immunol. 164: 5522-5529 [Abstract] [Full Text]  
• Lin, C.-L., Lee, J.-C., Chen, S.-S., Alan Wood, H., Li, M.-L., Li, C.-F., Chao, Y.-C. (1999). Persistent Hz-1 Virus Infection in Insect Cells: Evidence for Insertion of Viral DNA into Host Chromosomes and Viral Infection in a Latent Status. J. Virol. 73: 128-139 [Abstract] [Full Text]  
• Lee, J.-C., Chen, H.-H., Chao, Y.-C. (1998). Persistent Baculovirus Infection Results from Deletion of the Apoptotic Suppressor Gene p35. J. Virol. 72: 9157-9165 [Abstract] [Full Text]  
• Chao, Y.-C., Lee, S.-T., Chang, M.-C., Chen, H.-H., Chen, S.-S., Wu, T.-Y., Liu, F.-H., Hsu, E.-L., Hou, R. F. (1998). A 2.9-Kilobase Noncoding Nuclear RNA Functions in the Establishment of Persistent Hz-1 Viral Infection. J. Virol. 72: 2233-2245 [Abstract] [Full Text]  
• Fan, T., Lu, H., Hu, H., Shi, L., McClarty, G. A., Nance, D. M., Greenberg, A. H., Zhong, G. (1998). Inhibition of Apoptosis in Chlamydia-infected Cells: Blockade of Mitochondrial Cytochrome c Release and Caspase Activation. J. Exp. Med. 187: 487-496 [Abstract] [Full Text]  
• Wada, N., Matsumura, M., Ohba, Y., Kobayashi, N., Takizawa, T., Nakanishi, Y. (1995). Transcription Stimulation of the Fas-encoding Gene by Nuclear Factor for Interleukin-6 Expression upon Influenza Virus Infection. J. Biol. Chem. 270: 18007-18012 [Abstract] [Full Text]